Geographic variability of Escherichia coli ribotypes from animals in Idaho and Georgia.
Abstract: Several genotypic methods have been developed for determining the host origin of fecal bacteria in contaminated waters. Some of these methods rely on a host origin database to identify environmental isolates. It is not well understood to what degree these host origin isolates are geographically variable (i.e., cosmopolitan or endemic). This is important because a geographically limited host origin database may or may not be universally applicable. The objective of our study was to use one genotypic method, ribotyping, to determine the geographic variability of the fecal bacterium, Escherichia coli, from one location in Idaho and three locations in Georgia for cattle (Bos taurus), horse (Equus caballus), swine (Sus scrofa), and chicken (Gallus gallus domesticus). A total of 568 fecal E. coli isolates from Kimberly, ID (125 isolates), Athens, GA (210 isolates), Brunswick, GA (102 isolates), and Tifton, GA (131 isolates), yielded 213 ribotypes. The percentage of ribotype sharing within an animal species increased with decreased distance between geographic locations for cattle and horses, but not for swine and chicken. When the E. coli ribotypes among the four host species were compared at one location, the percent of unshared ribotypes was 86, 89, 81, and 79% for Kimberly, Athens, Brunswick, and Tifton, respectively. These data suggest that there is good ribotype separation among host animal species at each location. The ability to match environmental isolates to a host origin database may depend on a large number of environmental and host origin isolates that ideally are not geographically separated.
Publication Date: 2002-08-15 PubMed ID: 12175046DOI: 10.2134/jeq2002.1273Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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This research study explores the geographic variability of Escherichia coli ribotypes found in animals in Idaho and Georgia. It aims to determine whether the bacteria are cosmopolitan or endemic, and how this could impact the application of a host origin database utilized for fecal pollution detection in water.
Research Methodology
- The researchers adopted a genotypic method called ribotyping to track the geographic variation of Escherichia coli.
- Four different animal hosts were chosen for the study: cattle (Bos taurus), horse (Equus caballus), swine (Sus scrofa), and chicken (Gallus gallus domesticus).
- The study was conducted in one location in Idaho (Kimberly) and three locations in Georgia (Athens, Brunswick, and Tifton).
- A total of 568 fecal E. coli isolates were collected from the different locations and animals.
Findings
- The research found 213 different ribotypes among the E. coli isolates.
- The results showed that ribotype sharing within an animal species increased as the distance between geographic locations decreased for cattle and horses, but not for swine and chicken. This implies that the E. coli variations found in these animal species might be more influenced by their local environment.
- When comparing the E. coli ribotypes among the four host species at one location, a high percentage of ribotypes were unique to each host species, ranging from 79% to 89% for different locations. This suggests that there is a considerable ribotype separation among host animal species at each location.
Implications
- These findings suggest the importance of having a comprehensive and geographically diverse host origin database for identifying fecal contamination origin with more accuracy.
- The geographic variability of E. coli variations and its impact on the applicability of a host origin database should not be overlooked. It emphasizes that E. coli variations can be highly influenced by local environmental conditions, which suggests that a universally applicable database might need to account for these variations.
Cite This Article
APA
Hartel PG, Summer JD, Hill JL, Collins JV, Entry JA, Segars WI.
(2002).
Geographic variability of Escherichia coli ribotypes from animals in Idaho and Georgia.
J Environ Qual, 31(4), 1273-1278.
https://doi.org/10.2134/jeq2002.1273 Publication
Researcher Affiliations
- Dep of Crop and Soil Sciences, Univ of Georgia, Athens 30602-7272, USA. pghartel@arches.uga.edu
MeSH Terms
- Animals
- Cattle / microbiology
- Chickens / microbiology
- Databases, Factual
- Escherichia coli / genetics
- Escherichia coli / pathogenicity
- Genetic Variation
- Genotype
- Geography
- Georgia
- Horses / microbiology
- Idaho
- Ribotyping
- Swine / microbiology
Citations
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