Glucose transport and glycolytic enzyme activities in erythrocytes of two-year-old thoroughbreds undergoing training exercise.
Abstract: D-Glucose transport and cytosolic enzyme activities were measured in erythrocytes from 2-year-old thoroughbreds under continuous training exercise (race horses) and compared with those from untrained horses of various ages (sires, mares and untrained 2-year-old thoroughbreds). The activities of the glucose transport and glycolytic enzymes, hexokinase and pyruvate kinase, in the race horses' erythrocytes were elevated to 2-3.5 times above those of untrained horses. There were no significant differences in plasma glucose, triglyceride or IRI concentrations between the horses in training and untrained horses. The increases in glucose transport and glycolytic enzyme activities in their erythrocytes are considered to reflect an increased metabolic activity in the race horses resulting from the training exercises.
Publication Date: 1994-01-01 PubMed ID: 7701778DOI: 10.1007/BF01839417Google Scholar: Lookup
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- Journal Article
Summary
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The research paper examines the effect of continuous training exercises on the glucose transport and the activities of glycolytic enzymes in the blood cells of two-year-old thoroughbred racing horses, and compares them with those in untrained horses of various ages.
Objectives and Methodology
- This research aimed to investigate the impact of continuous training on glucose transport (the process by which glucose enters or leaves cells) and the activities of certain enzymes involved in glycolysis (the metabolic process that breaks down glucose to produce energy) in two-year-old racehorses. The researchers compared this data with similar measurements from untrained horses of various ages.
- The authors measured D-Glucose transport and cytosolic enzyme activities in the erythrocytes (red blood cells) from both trained and untrained horses.
- The glycolytic enzymes considered in this study included hexokinase and pyruvate kinase. These enzymes play a critical role in the process of glycolysis and could be indicative of the overall metabolic activity in the horses.
Findings
- The activities of glucose transport and the two glycolytic enzymes were found to be 2 to 3.5 times higher in racehorses’ erythrocytes than in untrained horses.
- In terms of plasma glucose, triglyceride or IRI concentrations, no significant differences were detected between horses in training and untrained horses. These results indicate that the change in metabolic activities due to training doesn’t significantly alter these constituents of the blood.
Key Outcome
- The research suggests that the increased activities of glucose transport and the glycolytic enzymes in the erythrocytes of racehorses reflect the enhanced metabolic activity resulting from the training exercises. This could mean that the training process itself may improve the efficiency of glucose utilization and energy production at the cellular level in racehorses.
Cite This Article
APA
Arai T, Washizu T, Hamada S, Sako T, Takagi S, Yashiki K, Motoyoshi S.
(1994).
Glucose transport and glycolytic enzyme activities in erythrocytes of two-year-old thoroughbreds undergoing training exercise.
Vet Res Commun, 18(6), 417-422.
https://doi.org/10.1007/BF01839417 Publication
Researcher Affiliations
- Department of Veterinary Science, Nippon Veterinary and Animal Science University, Tokyo, Japan.
MeSH Terms
- Animals
- Biological Transport
- Erythrocytes / enzymology
- Female
- Glucose / metabolism
- Hexokinase / metabolism
- Horses
- Male
- Physical Conditioning, Animal / physiology
- Pyruvate Kinase / metabolism
References
This article includes 15 references
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Citations
This article has been cited 1 times.- Arai T, Takahashi M, Araki K, Washizu T. Activities of enzymes related to the malate-aspartate shuttle in the blood cells of thoroughbred horses undergoing training exercise. Vet Res Commun 2001 Oct;25(7):577-83.
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