Glycosaminoglycan content of glomerular and tubular basement membranes of various mammalian species.
Abstract: A spectrophotometric assay was applied for quantitation of sulfated glycosaminoglycans in digested renal basement membranes of six mammalian species. The conditions of digestion and the accuracy of the assay were evaluated. Papain digestion and alkaline treatment appeared to be most effective for solubilization. Basement membrane preparations obtained by sonication contained more glycosaminoglycans than those isolated by detergent treatment. Glomerular basement membranes had generally a higher glycosaminoglycan content than tubular basement membranes.
Publication Date: 1985-01-28 PubMed ID: 3967044DOI: 10.1016/0304-4165(85)90260-0Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research has assessed the amount of sulfated glycosaminoglycans in the renal basement membranes of six different mammal species using a spectrophotometric test. The study’s key findings include the fact that papain digestion and alkaline treatment are the most effective solubilization methods, and that sonication yields more glycosaminoglycans than detergent treatment. Besides, the research found that glomerular basement membranes generally have a higher glycosaminoglycan content than tubular basement membranes.
Experiment Setup
- The study employed a spectrophotometric assay, a method used to measure the concentration of a given substance in a solution.
- The assay measured the amount of sulfated glycosaminoglycans present in the renal basement membranes of six different species.
- The renal basement membranes are thin, delicate structures within the kidneys which act as a filter separating the blood from other substances.
- Glycosaminoglycans are a type of complex carbohydrate structure found in these membranes.
Solubilization Techniques
- Two different methods were tried during the experiment to release the glycosaminoglycans from the basement membranes into a soluble form easier to measure: papain digestion and alkaline treatment.
- Papain digestion uses an enzyme called papain to break down the material where glycosaminoglycans are found.
- Alkaline treatment involves breaking down the sample using an alkaline solution.
- The study concluded that both techniques were effective, with the papain digestion and alkaline treatment methods proving to be particularly efficient.
Measuring Glycosaminoglycan Levels
- During the experiment, basement membrane samples were also prepared through sonication and detergent treatment.
- Sonication is a method that uses ultrasonic waves to break up or mix substances, while detergent treatment involves using a detergent to separate the membrane’s components.
- The study found that sonication provided a higher yield of glycosaminoglycans than detergent treatment.
Comparing Different Membranes
- The research investigated two types of basement membranes within the kidney: the glomerular and the tubular basement membranes.
- It was identified that the glomerular basement membranes had a higher concentration of glycosaminoglycans than the tubular basement membranes.
Cite This Article
APA
Reubsaet FA, Langeveld JP, Veerkamp JH.
(1985).
Glycosaminoglycan content of glomerular and tubular basement membranes of various mammalian species.
Biochim Biophys Acta, 838(1), 144-150.
https://doi.org/10.1016/0304-4165(85)90260-0 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Basement Membrane / analysis
- Cattle
- Deoxycholic Acid
- Glycosaminoglycans / analysis
- Horses
- Humans
- Hydrogen-Ion Concentration
- Kidney Glomerulus / analysis
- Kidney Tubules / analysis
- Papain
- Rabbits
- Sheep
- Solubility
- Sonication
- Spectrophotometry
- Swine
Citations
This article has been cited 6 times.- Jenner JR, Buttle DJ, Dixon AK. Mechanism of action of intradiscal chymopapain in the treatment of sciatica: a clinical, biochemical, and radiological study.. Ann Rheum Dis 1986 Jun;45(6):441-9.
- Vermylen C, Levin M, Mossman J, Barratt TM. Glomerular and urinary heparan sulphate in congenital nephrotic syndrome.. Pediatr Nephrol 1989 Apr;3(2):122-9.
- van den Heuvel LP, van den Born J, van de Velden TJ, Veerkamp JH, Monnens LA, Schroder CH, Berden JH. Isolation and partial characterization of heparan sulphate proteoglycan from the human glomerular basement membrane.. Biochem J 1989 Dec 1;264(2):457-65.
- Horner AA. Rat heparan sulphates. A study of the antithrombin-binding properties of heparan sulphate chains from rat adipose tissue, brain, carcase, heart, intestine, kidneys, liver, lungs, skin and spleen.. Biochem J 1990 Mar 1;266(2):553-9.
- Linshaw MA. Selected aspects of cell volume control in renal cortical and medullary tissue.. Pediatr Nephrol 1991 Sep;5(5):653-65.
- Van den Heuvel LP, Van den Born J, Jalanko H, Schröder CH, Veerkamp JH, Assmann KJ, Berden JH, Holmberg C, Rapola J, Monnens LA. The glycosaminoglycan content of renal basement membranes in the congenital nephrotic syndrome of the Finnish type.. Pediatr Nephrol 1992 Jan;6(1):10-5.
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