Gross, organoleptic and histologic assessment of cadaveric equine heads preserved using chemical methods for veterinary surgical teaching.
Abstract: Preservation of biological tissues has been used since ancient times. Regardless of the method employed, tissue preservation is thought to be a vital step in veterinary surgery teaching and learning. Objective: This study was designed to determine the usability of chemically preserved cadaveric equine heads for surgical teaching in veterinary medicine. Methods: Six cadaveric equine heads were collected immediately after death or euthanasia and frozen until fixation. Fixation was achieved by using a hypertonic solution consisting of sodium chloride, sodium nitrite and sodium nitrate, and an alcoholic solution containing ethanol and glycerin. Chemically preserved specimens were stored at low temperatures (2°C to 6°C) in a conventional refrigerator. The specimens were submitted to gross and organoleptic assessment right after fixative solution injection (D0) and within 10, 20, and 30 days of fixation (D10, D20, and D30, respectively). Samples of tissue from skin, tongue, oral vestibule, and masseter muscle were collected for histological evaluation at the same time points. Results: Physical and organoleptic assessments revealed excellent specimen quality (mean scores higher than 4 on a 5-point scale) in most cases. In some specimens, lower scores (3) were assigned to the range of mouth opening, particularly on D0 and D10. A reduced the range of mouth opening may be a limiting factor in teaching activities involving structures located in the oral cavity. Conclusions: The excellent physical, histologic, and organoleptic characteristics of the specimens in this sample support their usability in teaching within the time frame considered. Appropriate physical and organoleptic characteristics (color, texture, odor, and flexibility) of the specimens in this study support the use of the method described for preparation of reusable anatomical specimens.
© 2024 The Korean Society of Veterinary Science.
Publication Date: 2024-04-03 PubMed ID: 38568830PubMed Central: PMC10990915DOI: 10.4142/jvs.23176Google Scholar: Lookup
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- Journal Article
Summary
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The research article presents a study assessing the effectiveness of chemically preserving horse heads for veterinary surgical education. The study discovered that these chemically preserved specimens retain their physical and histologic characteristics sufficiently for teaching purposes, despite slight limitations noted in mouth opening in some cases.
Objectives and Methodology
- The study aimed to evaluate the practicality of using chemically preserved horse heads as teaching tools for veterinary medicine.
- Six horse heads were collected post-mortem and preserved by using a mixture of several chemicals (sodium chloride, sodium nitrite, sodium nitrate, ethanol, and glycerin).
- After the chemical treatment, specimens were stored at low temperatures within a refrigerator.
- These specimens underwent physical and sensory assessment immediately after chemical treatment, and again at 10, 20, and 30-day intervals.
- Additionally, tissue samples from the skin, tongue, oral vestibule, and masseter muscle were collected for histological analysis at the same intervals.
Results
- The results indicated that the specimens generally retained excellent quality (as per their approximately 4 out of 5 score), meeting the required criteria in most instances.
- However, some specimens scored lower (3 out of 5) on the range of mouth opening, particularly immediately after chemical treatment and at the 10-day mark, which could pose challenges to using these specimens for teaching oral procedures.
- Despite this, the specimens remained in good condition for teaching purposes within the 30-day period observed in the study.
Conclusion
- The study concluded that the effective preservation of physical, histologic, and sensory characteristics of the specimens demonstrate that the method used could work well for preparing reusable anatomical specimens.
- The specimens specifically showed adequate quality in terms of color, texture, odor, and flexibility, thus promoting their use in veterinary education.
Cite This Article
APA
Romero Corrêa R, Peres Mendes R, Darley Velasquez Piñeros D, De Lima AE, do Valle De Zoppa AL, Lopes Correia da Silva LC, de Francisco Strefezzi R, de Freitas SH.
(2024).
Gross, organoleptic and histologic assessment of cadaveric equine heads preserved using chemical methods for veterinary surgical teaching.
J Vet Sci, 25(2), e29.
https://doi.org/10.4142/jvs.23176 Publication
Researcher Affiliations
- Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP 05508-270, Brazil.
- Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP 05508-270, Brazil. rubensmendes@usp.br.
- Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP 05508-270, Brazil.
- Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP 05508-270, Brazil.
- Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP 05508-270, Brazil.
- Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP 05508-270, Brazil.
- Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP 13635-900, Brazil.
- Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP 13635-900, Brazil.
MeSH Terms
- Animals
- Horses
- Ethanol
- Sensation
- Cadaver
- Horse Diseases
Conflict of Interest Statement
The authors declare no conflicts of interest.
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