Growth hormone or insulin-like growth factor-I extends longevity of equine spermatozoa in vitro.
Abstract: Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are both present in blood plasma and IGF-I has been measured in epididymal fluid and seminal plasma. This study was designed to investigate the direct effects of GH or IGF-I on the motility of mature equine spermatozoa in vitro. We compared the effects of one concentration (100 ng/ml) of recombinant bovine GH (rbGH) and recombinant human IGF-I (rhIGF-I) on motility and motion characteristics of equine spermatozoa over a 24 h period. Motility was maintained longer in spermatozoa treated with either rbGH or rhIGF-I during a 24 h period at room temperature (P < 0.05). Spermatozoa motion characteristics at time 0, 1, 2, 4, 6, 12 and 24 h for both rbGH and rhlGF-I were not significantly different from the respective controls. This study has shown that GH and IGF-I are effective in promoting the in vitro longevity of spermatozoa.
Publication Date: 2002-06-04 PubMed ID: 12041683DOI: 10.1016/s0093-691x(02)00640-4Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigation demonstrates that growth hormone (GH) and insulin-like growth factor-I (IGF-I) can enhance the viability of horse sperm in laboratory settings.
Background and Aim
- In the realm of reproductive biology, professionals often study the potentials water-shedding hormones can provide.
- Two such hormones include Growth Hormone (GH) and Insulin-like Growth Factor-I (IGF-I), both of which circulate in the blood. Moreover, IGF-I has also been measured in the fluid from the epididymis and the semen.
- This investigation was designed to provide insights into the possible effects of GH and IGF-I in ensuring the motility of mature equine spermatozoa in controlled lab conditions.
Methodology & Findings
- The experiment compared how a specific concentration (100 ng/ml) of recombinant bovine GH (rbGH) and recombinant human IGF-I could affect the mobility and motion characteristics of equine sperm over a 24 hour period.
- The results demonstrated positive results where motility was substantially maintained in sperm that were treated with either rbGH or rhIGF-I over 24 hours at room temperature.
- The various aspects of sperm motion at various intervals such as 0, 1, 2, 4, 6, 12, and 24 hours for both rbGH and rhIGF-I treated samples exhibited no substantial divergence from their respective control samples.
Conclusion
- Overall, this study highlighted the beneficial influence of GH and IGF-I in sustaining the in vitro longevity of equine spermatozoa.
- Such findings can have significant implications for enhancing reproduction in horses and potentially other mammalian species.
Cite This Article
APA
Champion ZJ, Vickers MH, Gravance CG, Breier BH, Casey PJ.
(2002).
Growth hormone or insulin-like growth factor-I extends longevity of equine spermatozoa in vitro.
Theriogenology, 57(7), 1793-1800.
https://doi.org/10.1016/s0093-691x(02)00640-4 Publication
Researcher Affiliations
- Research Centre in Reproductive Medicine, Faculty of Medicine and Health Science, The University of Auckland, New Zealand. zahrachampion@hotmail.com
MeSH Terms
- Animals
- Cell Survival
- Horses
- Human Growth Hormone / pharmacology
- Insulin-Like Growth Factor I / pharmacology
- Male
- Semen Preservation / veterinary
- Specimen Handling / veterinary
- Sperm Motility / drug effects
- Spermatozoa / physiology
- Time Factors
Citations
This article has been cited 7 times.- Asimakopoulos B, Tiptiri-Kourpeti A, Metallinou C. IGF-I and NGFβ enhance in vitro progressive motility and vitality of human spermatozoa. Reprod Med Biol 2021 Jul;20(3):361-367.
- Zhang XD, Guo XY, Tang JX, Yue LN, Zhang JH, Liu T, Dong YX, Tang SS. The treatment effect of novel hGHRH homodimer to male infertility hamster. Korean J Physiol Pharmacol 2018 Nov;22(6):637-647.
- Hull KL, Harvey S. Growth hormone and reproduction: a review of endocrine and autocrine/paracrine interactions. Int J Endocrinol 2014;2014:234014.
- Yoon M, Jiang J, Chung KH, Roser JF. Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis. J Reprod Dev 2015;61(1):30-4.
- Magon N, Singh S, Saxena A, Sahay R. Growth hormone in male infertility. Indian J Endocrinol Metab 2011 Sep;15 Suppl 3(Suppl3):S248-9.
- Harvey S. Extrapituitary growth hormone. Endocrine 2010 Dec;38(3):335-59.
- Metallinou C, Staneloudi C, Nikolettos K, Asimakopoulos B. NGF, EPO, and IGF-1 in the Male Reproductive System. J Clin Med 2024 May 15;13(10).
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