Growth inhibitory effect of triclosan on equine and bovine Babesia parasites.
Abstract: We evaluated the growth inhibitory effect of triclosan, which has recently been reported to inhibit the growth of Plasmodium species and Toxoplasma gondii, on bovine and equine Babesia parasites in in vitro cultures The growth of Babesia bovis and B. bigemina was significantly inhibited in the presence of 100 microg/ml of triclosan, while B. caballi and B. equi were susceptible to as low as 50 microg/ml. Babesia bigemina and B. caballi were completely cleared as early as on the first and second day of the treatment, respectively. These parasites did not exhibit any growth in the subsequent five-day period of subculture without triclosan. Drug-treated parasites appeared pycnotic and atypically shaped, and ultrastructurally showed pronounced vacuolations, leading to complete destruction of parasites. Light microscopy showed that used concentrations of triclosan showed no toxicity against the host cells. The results suggest that triclosan can be used for chemotherapy of babesiosis.
Publication Date: 2003-04-11 PubMed ID: 12685641
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research paper investigates the efficacy of triclosan, a commonly used antibacterial and antifungal agent, in inhibiting the growth of Babesia parasites, pathogens that cause Babesiosis in livestock like horses and cows.
Research Findings
- The research tested the impact of triclosan, a chemical reported to constrain the growth of Plasmodium species and Toxoplasma gondii, on Babesia parasites found in cattle and horses.
- The impact of triclosan was evaluated in an in vitro culture, meaning the testing was conducted outside a living organism, within a controlled laboratory environment.
- The results displayed significant growth inhibition of Babesia bovis and B. bigemina when exposed to triclosan at a concentration of 100 micrograms per milliliter.
- Furthermore, B. caballi and B. equi, other species of Babesia parasites, showed susceptibility to triclosan concentrations as low as 50 micrograms per milliliter.
Effect of Triclosan Treatment on Parasites
- The studies revealed that B. bigemina and B. caballi were entirely eliminated as early as the first and second day of triclosan treatment, respectively.
- These parasites did not demonstrate any signs of growth during subsequent observation periods of five days, even after the removal of triclosan from their environment.
- Morphological alterations were observed in the parasites treated with triclosan. They appeared pycnotic (shrunken and condensed) and changed to atypical form.
- Changed ultrastructure and pronounced vacuolations (formation of small cavities or space within the cell) leading to the complete destruction of parasites were other notable observations.
Impact of Triclosan on Host Cells
- Under light microscopy examination, it was found that the concentrations of triclosan used in this study did not exhibit toxicity against the host cells.
- This is a crucial finding as it signifies that triclosan primarily targets the parasites without causing substantial harm to the cells of the host organism.
Conclusion and Future Implications
- Based on the outcomes, the research implies that triclosan could potentially be used for chemotherapy of Babesiosis, an infectious disease spread to animals by ticks.
- Future research could potentially explore how triclosan can be safely and effectively administrated to animals afflicted with Babesiosis.
Cite This Article
APA
Bork S, Yokoyama N, Matsuo T, Claveria FG, Fujisaki K, Igarashi I.
(2003).
Growth inhibitory effect of triclosan on equine and bovine Babesia parasites.
Am J Trop Med Hyg, 68(3), 334-340.
Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
MeSH Terms
- Animals
- Antiprotozoal Agents / pharmacology
- Babesia / drug effects
- Babesia / growth & development
- Babesia / ultrastructure
- Cattle / parasitology
- Horses / parasitology
- Microscopy, Electron
- Triclosan / pharmacology
Citations
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