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Home / Equine Research Bank / Front Microbiol / Gut Fungal Microbiome Responses to Natural Cryptosporidium I...
Frontiers in microbiology2022; 13; 877280; doi: 10.3389/fmicb.2022.877280

Gut Fungal Microbiome Responses to Natural Cryptosporidium Infection in Horses.

Abstract: It is critical to characterize changes in the structure and composition of the host fungal community in natural infection, because it gives the possible overview of gut microbiome in host homeostasis and disease progression. A total of 168 rectal fecal samples were collected and examined using nPCR. The positive samples were double-checked using 18S rDNA high-throughput sequencing. After confirmation, ITS high-throughput sequencing was utilized to investigate the fungal community's response to natural infection. Results showed that a total of three positive samples (1.79%) were identified with an increased abundance of fungi associated with health hazards, such as class Dothideomycetes, families, i.e., Cladosporiaceae, Glomerellaceae, and genera, i.e., , , , , and . On the contrary, taxa associated with favorable physiological effects on the host were shown to have the reverse impact, such as families, i.e., Psathyrellaceae, Pseudeurotiaceae and genera (, , and ). For the first time, we evaluated the condition of natural infection in horses in Wuhan, China, and discovered distinct variations in the fungal microbiome in response to natural infection. It might prompt a therapy or prevention strategy to apply specific fungal microorganisms that are probably responsible for decreased susceptibility or increased resistance to infection.
Copyright © 2022 Wang, Li, Chen, Kulyar, Duan, Li, Bhutta, Wu and Li.
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Publication Date: 2022-07-06 PubMed ID: 35875530PubMed Central: PMC9298756DOI: 10.3389/fmicb.2022.877280Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article explores how a natural Cryptosporidium infection affects the structure and composition of the fungal community in horses. The study found distinct changes in the fungal microbiome, including an increase in hazardous fungi and a decrease in beneficial fungi.

Study Methodology

  • The study gathered a total of 168 rectal fecal samples from horses for examination. These samples were then processed using nested PCR (nPCR) – a highly sensitive technique for detecting specific genetic material.
  • Positive samples, i.e., those showed signs of Cryptosporidium infection, were further verified using 18S rDNA high-throughput sequencing, a technique that allows for the identification of a broad range of microorganisms.
  • Once the infection was confirmed, ITS high-throughput sequencing was used to investigate the changes in the fungal community as a result of the infection. ITS sequencing is a widely used method for fungal identification and community profiling.

Research Findings

  • The results indicated the presence of Cryptosporidium infection in three samples, which equates to 1.79% of the total collected samples.
  • An increase was observed in the abundance of certain fungi known to present health hazards to the host. This included the fungal class Dothideomycetes, families Cladosporiaceae and Glomerellaceae, and certain genera.
  • Conversely, taxa (groups of one or more populations of organisms) that are typically associated with beneficial effects on the host displayed a decreased presence. This included families such as Psathyrellaceae and Pseudeurotiaceae and certain genera.

Implications of the Study

  • This is the first study to evaluate the state of natural Cryptosporidium infection in horses in Wuhan, China.
  • The distinct variations observed in the gut fungal microbiome in response to the infection could lead to improved therapeutic or prevention strategies.
  • The application of specific fungal microorganisms that demonstrate potential for decreasing susceptibility or increasing resistance to infection may be considered for future research or clinical application.

Cite This Article

APA
Wang Y, Li X, Chen X, Kulyar MF, Duan K, Li H, Bhutta ZA, Wu Y, Li K. (2022). Gut Fungal Microbiome Responses to Natural Cryptosporidium Infection in Horses. Front Microbiol, 13, 877280. https://doi.org/10.3389/fmicb.2022.877280

Publication

Frontiers in microbiology
ISSN: 1664-302X
NlmUniqueID: 101548977
Country: Switzerland
Language: English
Volume: 13
Pages: 877280
PII: 877280

Researcher Affiliations

Wang, Yaping
  • Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Li, Xuwen
  • Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Chen, Xiushuang
  • Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Kulyar, Muhammad Fakhar-E-Alam
  • College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Duan, Kun
  • China Tobacco Henan Industrial Co., Ltd., Zhengzhou, China.
Li, Huade
  • Sichuan Academy of Grassland Science, Chengdu, China.
Bhutta, Zeeshan Ahmad
  • Laboratory of Biochemistry and Immunology, College of Veterinary Medicine, Chungbuk National University, Cheongju, South Korea.
Wu, Yi
  • Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Li, Kun
  • Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
  • MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

Conflict of Interest Statement

KD was employed by China Tobacco Henan Industrial Co., Ltd., China. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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