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Veterinary parasitology2008; 156(3-4); 241-247; doi: 10.1016/j.vetpar.2008.05.031

Haplotypic variability within the mitochondrial gene encoding for the cytochrome c oxidase 1 (cox1) of Cylicocyclus nassatus (Nematoda, Strongylida): evidence for an affiliation between parasitic populations and domestic and wild equid hosts.

Abstract: This study investigated the genetic variability within Cylicocyclus nassatus (Nematoda, Strongylida, Cyathostominae) collected from different domestic and wild hosts (i.e. horse, donkey, Przewalskii horse, tarpan and Turkmen kulan) and localities in Europe and/or USA. The ribosomal Internal Transcribed Spacer 2 (ITS2) and the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were PCR-amplified and sequences characterized from seventy individual parasitic specimens. While ITS2 displayed 0-0.6% variation rate among all individual adult specimens of C. nassatus examined, 22 different sequence variants (haplotypes) of cox1 were detected. Nucleotide variation was detected at 75 of the total 689 positions (overall 10.8% rate of intraspecific nucletidic difference) in the cox1, with the absence of invariable positions among specimens collected from each equid species or country. Conversely, two haplotypes were detected in horses from USA and in donkeys of Italy and Ukraine, respectively. The absence of haplotypes shared by the equid species suggests an affiliation of C. nassatus populations to their specific host. The results of the present study demonstrated that the characterization of mitochondrial regions may have important implications for studying the genetic structure and biology of equine cyathostomes, and to exploit taxonomic issues and practical implications related to the spread of anthelmintic resistance.
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Publication Date: 2008-06-06 PubMed ID: 18619736DOI: 10.1016/j.vetpar.2008.05.031Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research presents a study on the genetic variability within a particular parasite found in both domestic and wild horses and highlights potential implications for studying the genetic structure of such parasites and understanding anthelmintic resistance.

Study Objectives and Methods

  • The research undertook an investigation into the genetic diversity of Cylicocyclus nassatus, a type of nematode, or worm, often found in equine species such as horses, donkey, and certain types of wild horses like tarpans and Turkmen kulans.
  • Equines from different locations in Europe and the USA were sampled for this study.
  • The study focused on two genetic regions of the worm: the Internal Transcribed Spacer 2 (ITS2) and a mitochondrial gene known as cytochrome c oxidase subunit 1 (cox1).
  • Seventy individual parasitic specimens were collected, and their ITS2 and cox1 gene sequences were PCR-amplified and characterized using genetic sequencing techniques.

Study Findings

  • Low genetic variation (0-0.6%) was observed in the ITS2 gene among all individual adult specimens of C. nassatus.
  • Contrarily, twenty-two different sequence variants or haplotypes of the cox1 gene were discovered.
  • These cox1 variations occurred at a significant rate of 10.8% at 75 out of 689 nucleotide positions analyzed.
  • Interestingly, no consistent patterns were found among specimens collected from each equid species or country, suggesting considerable genetic diversity within the parasite’s population.
  • However, two distinct haplotypes were identified specifically in horses from the USA and donkeys in Italy and Ukraine.
  • Consequently, none of the haplotypes were shared across the different equine species, indicating a potential link between the parasite populations and their specific hosts.

Implications

  • The observed genetic diversity within the cox1 gene of C. nassatus could be crucial in studying the genetic structure and biology of similar parasites (cyathostomes) occurring in equine species.
  • This could help in addressing taxonomic issues related to such equine parasites.
  • The findings also carry practical implications for understanding the spread of anthelmintic resistance, a global problem impacting the effective control of parasitic worms in animals.

Cite This Article

APA
Traversa D, Kuzmina T, Kharchenko VA, Iorio R, Klei TR, Otranto D. (2008). Haplotypic variability within the mitochondrial gene encoding for the cytochrome c oxidase 1 (cox1) of Cylicocyclus nassatus (Nematoda, Strongylida): evidence for an affiliation between parasitic populations and domestic and wild equid hosts. Vet Parasitol, 156(3-4), 241-247. https://doi.org/10.1016/j.vetpar.2008.05.031

Publication

Veterinary parasitology
ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 156
Issue: 3-4
Pages: 241-247

Researcher Affiliations

Traversa, Donato
  • Department of Comparative Biomedical Sciences, Faculty of Veterinary Medicine, Piazza Aldo Moro 45, 64100 Teramo, Italy. dtraversa@unite.it
Kuzmina, Tetyana
    Kharchenko, Vitaliy A
      Iorio, Raffaella
        Klei, Thomas R
          Otranto, Domenico

            MeSH Terms

            • Animals
            • DNA, Mitochondrial / genetics
            • DNA, Ribosomal Spacer
            • Electron Transport Complex IV / genetics
            • Equidae / parasitology
            • Haplotypes
            • Host-Parasite Interactions
            • Polymerase Chain Reaction
            • Strongylida / enzymology
            • Strongylida / genetics

            Citations

            This article has been cited 10 times.
            1. Gülbudak H, Ülger ST, Bozok T, Aslan G. Epidemiological and molecular characterization of Enterobius vermicularis cases in Mersin, Turkey: An analysis based on mitochondrial DNA cox1 gene sequences. One Health 2025 Jun;20:101079.
              doi: 10.1016/j.onehlt.2025.101079pubmed: 40496835google scholar: lookup
            2. Kuzmina TA, Königová A, Antipov A, Kuzmin Y, Kharchenko V, Syrota Y. Changes in equine strongylid communities after two decades of annual anthelmintic treatments at the farm level. Parasitol Res 2024 Nov 25;123(11):394.
              doi: 10.1007/s00436-024-08417-5pubmed: 39585485google scholar: lookup
            3. Hamad MH, Islam SI, Jitsamai W, Chinkangsadarn T, Naraporn D, Ouisuwan S, Taweethavonsawat P. Metabarcoding study to reveal the structural community of strongylid nematodes in domesticated horses in Thailand. BMC Vet Res 2024 Feb 24;20(1):70.
              doi: 10.1186/s12917-024-03934-ypubmed: 38395874google scholar: lookup
            4. Haghshenas M, Koosha M, Latifi A, Kazemirad E, Dehghan A, Nikmanesh B, Mowlavi G. Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It's potential to compare genetic variations based on mitochondrial DNA (cox1) gene. PLoS One 2023;18(2):e0281622.
              doi: 10.1371/journal.pone.0281622pubmed: 36758053google scholar: lookup
            5. Louro M, Kuzmina TA, Bredtmann CM, Diekmann I, de Carvalho LMM, von Samson-Himmelstjerna G, Krücken J. Genetic variability, cryptic species and phylogenetic relationship of six cyathostomin species based on mitochondrial and nuclear sequences. Sci Rep 2021 Apr 15;11(1):8245.
              doi: 10.1038/s41598-021-87500-8pubmed: 33859247google scholar: lookup
            6. Červená B, Hrazdilová K, Vallo P, Pafčo B, Fenyková T, Petrželková KJ, Todd A, Tagg N, Wangue N, Lux Hoppe EG, Moraes MFD, Lapera IM, de Souza Pollo A, de Albuquerque ACA, Modrý D. Diversity of Mammomonogamus (Nematoda: Syngamidae) in large African herbivores. Parasitol Res 2018 Apr;117(4):1013-1024.
              doi: 10.1007/s00436-018-5777-ypubmed: 29470712google scholar: lookup
            7. Bredtmann CM, Krücken J, Murugaiyan J, Kuzmina T, von Samson-Himmelstjerna G. Nematode Species Identification-Current Status, Challenges and Future Perspectives for Cyathostomins. Front Cell Infect Microbiol 2017;7:283.
              doi: 10.3389/fcimb.2017.00283pubmed: 28702376google scholar: lookup
            8. Traversa D, Veronesi F, Diakou A, Iorio R, Simonato G, Marcer F, Di Cesare A. Mitochondrial haplotypes of Aelurostrongylus abstrusus and Troglostrongylus brevior (Nematoda, Metastrongyloidea) from domestic and wild felids. Parasitol Res 2017 Apr;116(4):1227-1235.
              doi: 10.1007/s00436-017-5399-9pubmed: 28197762google scholar: lookup
            9. Diakou A, Di Cesare A, Barros LA, Morelli S, Halos L, Beugnet F, Traversa D. Occurrence of Aelurostrongylus abstrusus and Troglostrongylus brevior in domestic cats in Greece. Parasit Vectors 2015 Nov 14;8:590.
              doi: 10.1186/s13071-015-1200-zpubmed: 26577206google scholar: lookup
            10. Delgado C, García G. Coevolution between Contracaecum (Nematoda, Anisakidae) and Austrolebias (Cyprinodontiformes, Rivulidae) host-parasite complex from SW Atlantic coastal basins. Parasitol Res 2015 Mar;114(3):913-27.
              doi: 10.1007/s00436-014-4257-2pubmed: 25544701google scholar: lookup
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