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[Hemolytic properties of bacteria belonging to the Acinetobacter genus].

Abstract: Direct and intermediate hemolytic activity of 526 strains of Acinetobacter was investigated. Their ability to produce lipase and lecithinase was also studied. Measurements were performed parallely on human, horse, sheep and bovine erythrocytes. Direct hemolytic activity was exhibited by 16% of tested strains (17 out of 24 strains of A. haemolyticus). Human, sheep and bovine erythrocytes were useful for testing the hemolytic activity of Acinetobacter. The hemolysis was occurring faster and was visible more frequently during incubation at 37 degrees C. Indirect hemolytic activity was observed in 88% of strains. Over 90% of strains were lipolytic after 24-48 hours which was independent of incubation temperature (22 and 37 degrees C).
Publication Date: 1993-01-01 PubMed ID: 8189804
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  • English Abstract
  • Journal Article

Summary

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The research investigates the hemolytic properties of 526 strains of Acinetobacter bacteria and their ability to produce lipase and lecithinase. The study found that 16% of the strains showed direct hemolytic activity whereas 88% exhibited indirect hemolytic activity. Moreover, over 90% of these strains were found to be lipolytic after a duration of 24 to 48 hours.

Investigating Hemolytic Activity

  • The research focuses on the hemolytic capabilities of the Acinetobacter bacteria genus. Hemolytic activity refers to the capacity of a bacterium to rupture erythrocytes or red blood cells, which is a capability that some pathogenic bacteria possess.
  • The study found that out of the 526 Acinetobacter strains tested, about 16% (17 out of 24 strains) exhibited direct hemolysis. This means that these strains directly interact and break down red blood cells in humans, horses, sheep, and bovines.
  • The research also highlighted that the hemolytic activity was more prominently visible during incubation at a temperature of 37 degrees Celsius.

Observing Indirect Hemolytic Activity

  • In addition to direct hemolysis, the research also studied the cases of indirect hemolysis, where the bacteria produce toxins that then lead to the breakdown of red blood cells.
  • The research noted that indirect hemolytic activity was observed in a whopping 88% of the strains tested, providing significant evidence of these bacteria’s potential pathogenic properties.

Production of Lipase and Lecithinase

  • Another significant aspect of the study was the investigation of the Acinetobacter strains’ ability to produce lipase and lecithinase. These are enzymes that break down fats and lecithin respectively, which are integral constituents of cell membranes.
  • The study revealed that over 90% of the tested strains were lipolytic (able to break down fats) after an incubation period of 24 to 48 hours. This observed activity was unaffected by the incubation temperature, showing similar results at both 22 and 37 degrees Celsius.

Cite This Article

APA
Gospodarek E. (1993). [Hemolytic properties of bacteria belonging to the Acinetobacter genus]. Med Dosw Mikrobiol, 45(3), 317-322.

Publication

ISSN: 0025-8601
NlmUniqueID: 0210575
Country: Poland
Language: pol
Volume: 45
Issue: 3
Pages: 317-322

Researcher Affiliations

Gospodarek, E
  • Katedra i Zakład Mikrobiologii AM, Bydgoszczy.

MeSH Terms

  • Acinetobacter / enzymology
  • Acinetobacter / immunology
  • Animals
  • Cattle
  • Erythrocytes / immunology
  • Hemolysis / physiology
  • Horses
  • Humans
  • Lipase / biosynthesis
  • Lipolysis
  • Phospholipases / biosynthesis
  • Sheep
  • Species Specificity
  • Temperature

Citations

This article has been cited 1 times.
  1. Tayabali AF, Nguyen KC, Shwed PS, Crosthwait J, Coleman G, Seligy VL. Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources.. PLoS One 2012;7(5):e37024.
    doi: 10.1371/journal.pone.0037024pubmed: 22655033google scholar: lookup