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PloS one2020; 15(11); e0241442; doi: 10.1371/journal.pone.0241442

Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF.

Abstract: Heterotopic and orthotopic ovarian tissue autotransplantation techniques, currently used in humans, will become promising alternative methods for fertility preservation in domestic and wild animals. Thus, this study describes for the first time the efficiency of a heterotopic ovarian tissue autotransplantation technique in a large livestock species (i.e., horses) after ovarian fragments were exposed or not to a cooling process (4°C/24 h) and/or VEGF before grafting. Ovarian fragments were collected in vivo via an ultrasound-guided biopsy pick-up method and surgically autografted in a subcutaneous site in both sides of the neck in each mare. The blood flow perfusion at the transplantation site was monitored at days 2, 4, 6, and 7 post-grafting using color-Doppler ultrasonography. Ovarian grafts were recovered 7 days post-transplantation and subjected to histological analyses. The exposure of the ovarian fragments to VEGF before grafting was not beneficial to the quality of the tissue; however, the cooling process of the fragments reduced the acute hyperemia post-grafting. Cooled grafts compared with non-cooled grafts contained similar values for normal and developing preantral follicles, vessel density, and stromal cell apoptosis; lower collagen type III fibers and follicular density; and higher stromal cell density, AgNOR, and collagen type I fibers. In conclusion, VEGF exposure before autotransplantation did not improve the quality of grafted tissues. However, cooling ovarian tissue for at least 24 h before grafting can be beneficial because satisfactory rates of follicle survival and development, stromal cell survival and proliferation, as well as vessel density, were obtained.
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Publication Date: 2020-11-04 PubMed ID: 33147235PubMed Central: PMC7641372DOI: 10.1371/journal.pone.0241442Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the effects of cooling and VEGF (Vascular Endothelial Growth Factor) on the autotransplantation of ovarian tissue in a large animal model, specifically horses. It concludes that cooling the tissue before transplantation can yield satisfactory outcomes, but VEGF exposure doesn’t enhance the quality of the grafted tissues.

Research Objectives and Methods

  • The study primarily aimed to assess the efficiency of a heterotopic ovarian tissue autotransplantation method in horses. The researchers further investigated if the exposure of ovarian fragments to a cooling process or VEGF yielded differing results prior to grafting.
  • Ovarian tissue fragments were extracted in vivo using an ultrasound-guided biopsy pick-up method and autografted subcutaneously in the neck of each horse.
  • Post-grafting, the blood flow at the transplantation site was monitored at days 2, 4, 6, and 7 using color-Doppler ultrasonography.
  • The ovarian grafts were then recovered 7 days post-transplantation and subjected to histological analyses.

Findings

  • VEGF exposure prior to grafting did not have a notable positive effect on tissue quality.
  • However, the cooling process of the ovarian tissue seemed to reduce acute hyperemias post-grafting. Acute hyperemia refers to a surge of blood in the body part upon which the grafting occurred.
  • When compared to non-cooled grafts, cooled grafts had similar values for normal and developing preantral follicles, vessel density, and stromal cell apoptosis (programmed cell death).
  • Moreover, cooled grafts had lower collagen type III fiber and follicular densities – showing the reduced formation of connective tissue post grafting – and higher densities of stromal cells (the supportive framework of an organ), AgNOR (a marker of cell proliferation), and collagen type I fibers.

Conclusions

  • The research concludes that the exposure to VEGF before autotransplantation does not improve the quality of grafted tissues in horses.
  • However, cooling the ovarian tissue for at least 24 hours before grafting can be beneficial as it produced satisfactory rates of follicle survival and development, stromal cell survival and proliferation, as well as vessel density.
  • The findings of the study have potential implications for fertility preservation not only in livestock but possibly in humans as well.

Cite This Article

APA
Souza SS, Alves BG, Alves KA, Brandão FAS, Brito DCC, Gastal MO, Rodrigues APR, Figueireod JR, Teixeira DIA, Gastal EL. (2020). Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF. PLoS One, 15(11), e0241442. https://doi.org/10.1371/journal.pone.0241442

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 15
Issue: 11
Pages: e0241442
PII: e0241442

Researcher Affiliations

Souza, Samara S
  • Laboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Alves, Benner G
  • Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Alves, Kele A
  • Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Brandão, Fabiana A S
  • Laboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Brito, Danielle C C
  • Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Gastal, Melba O
  • Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, United States of America.
Rodrigues, Ana P R
  • Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Figueireod, José R
  • Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Teixeira, Dárcio I A
  • Laboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, Brazil.
Gastal, Eduardo L
  • Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, United States of America.

MeSH Terms

  • Animals
  • Apoptosis / drug effects
  • Caspase 3 / metabolism
  • Cell Count
  • Cell Proliferation / drug effects
  • Cold Temperature
  • Female
  • Fibrosis
  • Horses
  • Models, Animal
  • Ovarian Follicle / blood supply
  • Ovarian Follicle / drug effects
  • Ovarian Follicle / transplantation
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
  • Regional Blood Flow / drug effects
  • Stromal Cells / cytology
  • Stromal Cells / drug effects
  • Transplantation, Autologous
  • Transplantation, Heterotopic
  • Vascular Endothelial Growth Factor A / pharmacology

Conflict of Interest Statement

The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.

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Citations

This article has been cited 3 times.
  1. Brandão FA, de Brito DC, Pereira LM, Alves KA, Ñaupas LV, de Souza SS, de S Cunha DM, de S Filho RP, Alves BG, Rodrigues AP, Teixeira DI. Effects of different subcutaneous sites on heterotopic autotransplantation of canine ovarian tissue. Vet Res Commun 2023 May 18;.
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  2. Souza SS, Aguiar FLN, Alves BG, Alves KA, Brandão FAS, Brito DCC, Raposo RDS, Gastal MO, Rodrigues APR, Figueiredo JR, Teixeira DÍA, Gastal EL. Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF. Reprod Fertil 2021 Dec;2(4):251-266.
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  3. . Correction: Heterotopic autotransplantation of ovarian tissue in a large animal model: Effects of cooling and VEGF. PLoS One 2021;16(5):e0252699.
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