High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres.
Abstract: The combined solid-phase extraction (Isolute PRS columns) and reversed-phase gradient HPLC method presented provides a sensitive, reproducible and selective quantification of carnosine, balenine, homocarnosine, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres. Recoveries were 91-115%. Lower limits of detection were 0.005-0.010 mmol kg-1 dry muscle. The compounds were isolated from other physiological amino acids and small peptides and resolved within a single chromatographic run of 55 min. Concentrations of these compounds in equine myocardium, diaphragm, skeletal muscle, camel muscle and individual muscle fibres of both species are presented for the first time.
Publication Date: 1997-01-10 PubMed ID: 9029312DOI: 10.1016/s0378-4347(97)88054-1Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article presents a method to quantify specific compounds in equine and camel muscle. This method uses solid-phase extraction and HPLC, showcasing high sensitivity, reproducibility, and selection. It also provides first-time data on concentrations of these compounds in various muscles of horses and camels.
Research Methodology
The researchers used a combined method of solid-phase extraction (Isolute PRS columns) and reversed-phase gradient high-performance liquid chromatography (HPLC). This approach was aimed at quantifying six compounds, namely carnosine, balenine, homocarnosine, histidine, 1-methylhistidine, and 3-methylhistidine, in equine and camel muscle as well as individual muscle fibres.
The features of the method included:
- Sensitive, reproducible, and selective quantification of the six compounds
- Recoveries ranged between 91% and 115% which indicates the efficiency of extraction and detection methods
- Lower limits of detection were within 0.005-0.010 mmol kg-1 dry muscle, emphasizing the sensitivity of the approach
- Isolation of the compound from other physiological amino acids and small peptides, ensuring specific detection
- A single chromatographic run of 55 minutes, highlighting the time efficiency of the method
Outcome and Significance
This research resulted in the first-ever presentation of concentrations of these compounds in equine myocardium, diaphragm, skeletal muscle, camel muscle, and individual muscle fibres of both species.
This is significant because:
- It adds to the scientific understanding of the biochemical composition of equine and camel muscles
- The method could be used in further research to understand the role of these compounds in muscle function and performance
- The data could be valuable for veterinarians, animal physiologists, and individuals involved in horse and camel racing for optimizing animal health and performance
Cite This Article
APA
Dunnett M, Harris RC.
(1997).
High-performance liquid chromatographic determination of imidazole dipeptides, histidine, 1-methylhistidine and 3-methylhistidine in equine and camel muscle and individual muscle fibres.
J Chromatogr B Biomed Sci Appl, 688(1), 47-55.
https://doi.org/10.1016/s0378-4347(97)88054-1 Publication
Researcher Affiliations
- Equine Sports Medicine Unit, Royal Veterinary College, North Mymms, Hertfordshire, UK.
MeSH Terms
- Animals
- Anserine / analysis
- Camelus / metabolism
- Carnosine / analogs & derivatives
- Carnosine / analysis
- Chromatography, High Pressure Liquid / methods
- Chromatography, High Pressure Liquid / veterinary
- Circadian Rhythm
- Dipeptides / analysis
- Female
- Histidine / analogs & derivatives
- Histidine / analysis
- Horses / metabolism
- Imidazoles / chemistry
- Male
- Methylhistidines / analysis
- Muscles / chemistry
- Muscles / pathology
- Osmolar Concentration
- Reproducibility of Results
- Sensitivity and Specificity
- Spectrometry, Fluorescence / veterinary
Citations
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