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Home / Equine Research Bank / J Antimicrob Chemother / High prevalence of international ESBL CTX-M-15-producing Ent...
The Journal of antimicrobial chemotherapy2016; 71(6); 1497-1500; doi: 10.1093/jac/dkw006

High prevalence of international ESBL CTX-M-15-producing Enterobacter cloacae ST114 clone in animals.

Abstract: The objective of this study was to characterize ESBL-producing Enterobacter cloacae isolated from animals and to compare their clonal distribution with that of human-related isolates. Among 635 clinical E. cloacae from horses, dogs and cats collected in France between 2010 and 2013, 36 were resistant to ceftiofur as determined by disc diffusion. ESBL genes were identified by sequencing. Plasmids carrying ESBL-encoding genes were characterized by PCR-based replicon typing, S1-PFGE and Southern blotting. IncHI2 plasmids were subtyped using the plasmid double-locus sequence typing scheme and multiplex amplification of the hipA, smr0092 and smr0183 genes. All E. cloacae were typed by PFGE and MLST. ST clustering was analysed by eBURST. All 36 ceftiofur-resistant E. cloacae produced an ESBL. Their PFGE patterns formed 23 clusters of high similarity and 13 STs and were isolated from epidemiologically unrelated animals (14 horses, 11 dogs and 11 cats) distributed throughout France. ST114, the most prevalent clone in humans, was over-represented in animals (16/36) compared with other human-related clones detected here. The blaCTX-M-15 gene was dominant (66.7%) and mostly carried on IncHI2 plasmids (ST1 subtype). ST114 isolates always produced CTX-M-15. Most ESBL-producing E. cloacae from animals studied here (69.4%) belonged to potentially high-risk clones in humans, in particular ST114 (44.4%). These data raise questions and potential concerns about the transfer of E. cloacae between animals and humans.
© The Author 2016. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
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Publication Date: 2016-02-04 PubMed ID: 26850718DOI: 10.1093/jac/dkw006Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research analysed Extended-Spectrum B-Lactamases (ESBL)-producing Enterobacter cloacae bacteria in animals and found a high frequency of clones linked to human infections. It brings attention to the potential danger of bacteria transfer between animals and humans.

Objective

The primary aim of this research was to investigate the existence and distribution of ESBL-producing Enterobacter cloacae in animal clinical isolates and compare these to clones found in human infections.

Methodology

  • A total of 635 clinical isolates from dogs, cats, and horses were tested for resistance to ceftiofur, an antibiotic, through disc diffusion.
  • The required ESBL genes were recognized on sequenced genomes.
  • The genomes carrying ESBL-encoding genes were further analyzed using a variety of techniques, including PCR-based replicon typing, S1-PFGE, Southern blotting, and sequence typing scheme.
  • All the E. cloacae types were differentiated with different typing methods such as MLST and PFGE.
  • The clustering of different STs was analyzed using eBURST.

Findings

  • It was found that all 36 ceftiofur-resistant E. cloacae released an ESBL.
  • The 36 isolates exhibited various PFGE patterns, forming 23 distinctive clusters.
  • These isolates were found distributed across France, coming from different animals, including 14 horses, 11 dogs, and 11 cats.
  • The ST114 clone, which is prevalent in human infections, was abundantly found in animals.
  • The blaCTX-M-15 gene, frequently carried on IncHI2 plasmids, was the most dominant, especially in ST114 isolates.
  • Almost 70% of the ESBL-producing E. cloacae from the animals belonged to ST clones that are potentially high-risk for humans, especially ST114.

Conclusion

The study indicates a potentially high risk of transfer of E. cloacae bacteria from animals to humans, particularly concerning the ST114 clone. The findings may help in future studies and surveillance to prevent bacterial transfer and the potential emergence of antibiotic-resistant strains.

Cite This Article

APA
Haenni M, Saras E, Ponsin C, Dahmen S, Petitjean M, Hocquet D, Madec JY. (2016). High prevalence of international ESBL CTX-M-15-producing Enterobacter cloacae ST114 clone in animals. J Antimicrob Chemother, 71(6), 1497-1500. https://doi.org/10.1093/jac/dkw006

Publication

The Journal of antimicrobial chemotherapy
ISSN: 1460-2091
NlmUniqueID: 7513617
Country: England
Language: English
Volume: 71
Issue: 6
Pages: 1497-1500

Researcher Affiliations

Haenni, Marisa
  • Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France marisa.haenni@anses.fr.
Saras, Estelle
  • Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
Ponsin, Cécile
  • Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
Dahmen, Safia
  • Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.
Petitjean, Marie
  • Laboratoire d'Hygiène Hospitalière, Centre Hospitalier Universitaire, Laboratoire Chrono-environnement UMR CNRS 6249, Univ. Bourgogne Franche-Comté, F-25000 Besançon, France.
Hocquet, Didier
  • Laboratoire d'Hygiène Hospitalière, Centre Hospitalier Universitaire, Laboratoire Chrono-environnement UMR CNRS 6249, Univ. Bourgogne Franche-Comté, F-25000 Besançon, France.
Madec, Jean-Yves
  • Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Antibiorésistance et Virulence Bactériennes, Lyon, France.

MeSH Terms

  • Animals
  • Blotting, Southern
  • Cats
  • Disk Diffusion Antimicrobial Tests
  • Dogs
  • Electrophoresis, Gel, Pulsed-Field
  • Enterobacter cloacae / classification
  • Enterobacter cloacae / enzymology
  • Enterobacter cloacae / genetics
  • Enterobacter cloacae / isolation & purification
  • Enterobacteriaceae Infections / epidemiology
  • Enterobacteriaceae Infections / microbiology
  • Enterobacteriaceae Infections / veterinary
  • France / epidemiology
  • Genotype
  • Molecular Epidemiology
  • Molecular Typing
  • Multilocus Sequence Typing
  • Plasmids / analysis
  • Plasmids / classification
  • Prevalence
  • Sequence Analysis, DNA
  • beta-Lactamases / analysis

Citations

This article has been cited 20 times.
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