Histologic and ultrastructural changes after large-colon torsion, with and without use of a specific platelet-activating factor antagonist (WEB 2086), in ponies.
Abstract: The role of platelet-activating factor (PAF) in mediating the colonic damage that develops after large-colon torsion was studied in 14 ponies. Morphologic changes in areas of the ascending colon and selected abdominal and thoracic viscera after 1 hour of large-colon torsion and 3 to 5 hours of reperfusion were determined, as well as the protective effects of systemic administration of a specific PAF antagonist (WEB 2086). Ponies were selected then allocated at random and in equal numbers to 2 groups that received 1 of 2 treatments prior to induction of large-colon torsion: group 1--control (saline solution), and group 2--WEB 2086 (3 mg/kg of body weight loading dose and 3 mg/kg/h for the remainder of the study). In each pony, full-thickness tissue specimens from the gastrointestinal tract--cecum, pelvic flexure, left and right ventral colon, and right dorsal colon--heart, left lung, liver, left adrenal gland, spleen, and right kidney were collected and histologically evaluated. Edema, mucosal necrosis, and neutrophil infiltration in colonic sections were graded from 0 (normal) to 3 (most severe changes). Sections of liver and lung from 3 ponies in each group, and colon from 1 pony in each group, also were examined by transmission electron microscopy to determine the presence of ultrastructural alterations. Ischemia and reperfusion induced marked changes in all sections of colon in all ponies: moderate to severe submucosal edema, moderate necrosis of the superficial epithelium and lamina propria, and necrosis of the mucosal crypt epithelium. Extravascular neutrophil accumulation was evident in all sections of colon and cecum, but not in other tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1994-05-01 PubMed ID: 8067617
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research article is a study about the effects of a specific platelet-activating factor antagonist (WEB 2086) in combating damage to the colon that results from large-colon torsion in ponies.
Background
- The research aimed to understand the role of platelet-activating factor (PAF) in causing colonic damage when a pony experiences large-colon torsion, a condition in which part of the colon twists upon itself, disrupting blood flow.
- Through histological and ultrastructural studies, the study sought to determine the extent of morphological changes in the colon and other selected viscera after one hour of torsion and a reperfusion period of 3 to 5 hours.
Methodology
- Fourteen ponies were split into two equal groups and each group received one of two treatments before a large-colon torsion was artificially induced.
- The control group received a saline solution, while the other group was administered WEB 2086, the PAF antagonist.
- From multiple locations in each pony’s body, full-thickness tissue specimens were collected and histologically evaluated. These included the gastrointestinal tract (various colon sections and the cecum) and many other organs like the heart, lung, liver, adrenal gland, spleen, and kidney.
- Assessments of colonics damage, such as edema, mucosal necrosis, and neutrophil infiltration, were graded from 0 (normal) to 3 (most severe changes).
- To further analyze the presence of ultrastructural alterations, sample sections from the liver and lung of three ponies, and the colon of one, from each group were examined using transmission electron microscopy.
Findings
- The occurrence of large-colon torsion and reperfusion led to significant changes in all sections of the colon in all ponies. These changes included moderate to severe submucosal edema, superficial epithelium necrosis, lamina propria necrosis, and mucosal crypt epithelium necrosis.
- A conspicuous accumulation of extravascular neutrophils, a type of white blood cell, was noticed in all sections of colon and cecum, but not in other tissues.
Cite This Article
APA
Wilson DV, Patterson JS, Stick JA, Provost PJ.
(1994).
Histologic and ultrastructural changes after large-colon torsion, with and without use of a specific platelet-activating factor antagonist (WEB 2086), in ponies.
Am J Vet Res, 55(5), 681-688.
Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824.
MeSH Terms
- Animals
- Azepines / therapeutic use
- Colon / drug effects
- Colon / pathology
- Colon / ultrastructure
- Colonic Diseases / pathology
- Colonic Diseases / prevention & control
- Colonic Diseases / veterinary
- Edema
- Epithelium / drug effects
- Epithelium / pathology
- Epithelium / ultrastructure
- Female
- Horse Diseases
- Horses
- Intestinal Mucosa / drug effects
- Intestinal Mucosa / pathology
- Intestinal Mucosa / ultrastructure
- Male
- Microscopy, Electron
- Necrosis
- Neutrophils / pathology
- Platelet Activating Factor / antagonists & inhibitors
- Torsion Abnormality
- Triazoles / therapeutic use
Citations
This article has been cited 1 times.- Faleiros RR, Macoris DG, Alves GE, Souza DG, Teixeira MM, Moore RM. Local and remote lesions in horses subjected to small colon distension and decompression.. Can J Vet Res 2008 Jan;72(1):68-76.
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