Histopathological Study of Esophageal Infection with Gasterophilus pecorum (Diptera: Oestridae) in Persian Onager (Equus hemionus onager).
Abstract: The larval stages of are obligate parasites in the gastrointestinal tract of equine accountable for pathologic ulcers in the Persian onager gastrointestinal. The aim of the current report was to study the histopathological change with larvae in the esophagus of a Persian onager. Methods: This study was performed in Iranian Zebra propagation and breeding site in Khartouran National Park, southeast of Shahrud City, Semnan Province, Iran in 2014. Following a necropsy with specific refer to esophagus of one adult female Persian onager were transmitted to the laboratory. After autopsy, parasites collected from the esophagus were transmitted into 70% alcohol. For histopathological investigation, tissue samples were collected from the esophagus. The tissues were fixed in 10% buffered formalin, and conformity routine processing, there were stained with Hematoxylin and eosin. Results: After clarity by lactophenol parasites were identified as . Microscopic recognition contained hyperemia, inflammatory cell infiltration, epithelial destruction, esophageal gland hyperplasia. Conclusions: This is the first survey of and histopathological study in the Persian onager esophagus in the world.
Publication Date: 2017-09-08 PubMed ID: 29322061PubMed Central: PMC5758640
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Summary
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The research article deals with the study of esophageal infection caused by the larvae of Gasterophilus pecorum, a type of parasite, in the Persian Onager, a subspecies of wild ass. The objective is to investigate histopathological changes in the esophagus of the affected animal.
Research Methodology
- The study was conducted at the Iranian Zebra propagation and breeding site situated in Khartouran National Park, southeast of Shahrud City, Semnan Province, Iran in the year 2014.
- The researchers performed a necropsy, an autopsy performed on an animal, with a particular focus on the esophagus of a mature female Persian Onager. Post-autopsy, the esophagus was brought to the lab for further analysis.
- The parasites identified in the esophagus were preserved in 70% alcohol to maintain their structural integrity for microscopic study and identification.
- For the histopathological investigation, tissue samples from the esophagus were collected. These samples were preserved in 10% buffered formalin, a solution used in preserving biological specimens.
- The processed tissues were stained with Hematoxylin and eosin, a combined staining technique used in the study of tissue under the microscope.
Findings and Conclusions
- The parasites, extracted and preserved from the esophagus, were identified as Gasterophilus pecorum, following clarification in a lactophenol solution.
- Microscopic analysis revealed a variety of pathological changes in the esophageal tissue such as hyperemia (an excess of blood in the vessels), inflammatory cell infiltration, epithelial destruction, and esophageal gland hyperplasia (an abnormal multiplication or growth of the esophageal gland’s cells).
- The study concluded that this is the first report of Gasterophilus pecorum and its associated histopathological changes in the esophagus of a Persian Onager.
- This research adds important data to the studies related to parasitic infections in animal species and could lead to better management, treatment, and prevention strategies for these infections in the Persian Onager population.
Cite This Article
APA
Hoseini SM, Zaheri BA, Adibi MA, Ronaghi H, Moshrefi AH.
(2017).
Histopathological Study of Esophageal Infection with Gasterophilus pecorum (Diptera: Oestridae) in Persian Onager (Equus hemionus onager).
J Arthropod Borne Dis, 11(3), 441-445.
Publication
Researcher Affiliations
- Department of Pathobiology, Babol Branch, Islamic Azad University, Babol, Iran.
- Department of Environmental Protection Semnan, Semnan, Iran.
- Department of Environmental Protection Semnan, Semnan, Iran.
- Department of Microbiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
- Young Researchers and Elite Club, Babol Branch, Islamic Azad University, Babol, Iran.
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Citations
This article has been cited 4 times.- Zhang K, Zhou R, Huang H, Ma W, Qi Y, Li B, Zhang D, Li K, Chu H. Host feces, olfactory beacon guiding aggregation of intestinal parasites Gasterophilus pecorum (Diptera: Gasterophilidae).. Parasitol Res 2022 Sep;121(9):2601-2613.
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- El-Hawagry MSA, Abdel-Dayem MS, Dhafer HMA. The family Oestridae in Egypt and Saudi Arabia (Diptera, Oestroidea).. Zookeys 2020;947:113-142.
- Li XY, Pape T, Zhang D. Taxonomic review of Gasterophilus (Oestridae, Gasterophilinae) of the world, with updated nomenclature, keys, biological notes, and distributions.. Zookeys 2019;891:119-156.
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