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Home / Equine Research Bank / J R Soc Interface / Hyaluronan supplementation as a mechanical regulator of cart...
Journal of the Royal Society, Interface2017; 14(133); 20170255; doi: 10.1098/rsif.2017.0255

Hyaluronan supplementation as a mechanical regulator of cartilage tissue development under joint-kinematic-mimicking loading.

Abstract: Articular cartilage plays an essential role in joint lubrication and impact absorption. Through this, the mechanical signals are coupled to the tissue's physiological response. Healthy synovial fluid has been shown to reduce and homogenize the shear stress acting on the cartilage surfaces due to its unique shear-thinning viscosity. As cartilage tissues are sensitive to mechanical changes in articulation, it was hypothesized that replacing the traditional culture medium with a healthy non-Newtonian lubricant could enhance tissue development in a cartilage engineering model, where joint-kinematic-mimicking mechanical loading is applied. Different amounts of hyaluronic acid were added to the culture medium to replicate the viscosities of synovial fluid at different health states. Hyaluronic acid supplementation, especially at a physiologically healthy concentration (2.0 mg ml), promoted a better preservation of chondrocyte phenotype. The ratio of collagen II to collagen I mRNA was 4.5 times that of the control group, implying better tissue development (however, with no significant difference of measured collagen II content), with a good retention of collagen II and proteoglycan in the mechanically active region. Simulating synovial fluid properties by hyaluronic acid supplementation created a favourable mechanical environment for mechanically loaded constructs. These findings may help in understanding the influence of joint articulation on tissue homeostasis, and moreover, improve methods for functional cartilage tissue engineering.
© 2017 The Author(s).
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Publication Date: 2017-08-05 PubMed ID: 28768880PubMed Central: PMC5582122DOI: 10.1098/rsif.2017.0255Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the role of hyaluronic acid in enhancing cartilage tissue development under conditions that mimic joint kinematics. Results suggest hyaluronic acid supplementation, particularly at concentrations observed in healthy joints, helps preserve the cartilage cell (chondrocyte) phenotype, encouraging better tissue development.

Introduction

  • The researchers acknowledged the critical role of articular cartilage in joint lubrication and impact absorption. They further highlighted that mechanical signals are vital for the physiological response of this tissue.
  • Notably, healthy synovial fluid reduces and evenly distributes the shear stress acting on the cartilage surfaces due to its unique shear-thinning viscosity property.
  • This led the team to suppose that including a healthy non-Newtonian lubricant in place of the traditional culture medium could improve tissue development in a cartilage engineering model, under conditions that mimic joint kinematics.

Methodology

  • The researchers used hyaluronic acid in varying amounts added to the culture medium, aiming to imitate the viscosities of synovial fluid at different health states.

Results

  • Hyaluronic acid supplementation, especially at a concentration of 2.0 mg/ml (physiologically healthy), promoted better preservation of the chondrocyte phenotype, according to the results.
  • This better preservation indicated an improvement in tissue development, with the ratio of collagen II (a primary component of cartilage) to collagen I mRNA being 4.5 times that of the control group.
  • Although there was no significant difference in measured collagen II content, there was improved retention of collagen II and proteoglycan (another critical component of cartilage) in the mechanically active region.

Conclusions

  • The supplementation of hyaluronic acid was found to create a favourable mechanical environment for mechanically loaded constructs, simulating the properties of synovial fluid.
  • The researchers highlight these findings could improve understanding regarding the influence of joint articulation on tissue homeostasis and may enhance methods for functional cartilage tissue engineering.

Cite This Article

APA
Wu Y, Stoddart MJ, Wuertz-Kozak K, Grad S, Alini M, Ferguson SJ. (2017). Hyaluronan supplementation as a mechanical regulator of cartilage tissue development under joint-kinematic-mimicking loading. J R Soc Interface, 14(133), 20170255. https://doi.org/10.1098/rsif.2017.0255

Publication

Journal of the Royal Society, Interface
ISSN: 1742-5662
NlmUniqueID: 101217269
Country: England
Language: English
Volume: 14
Issue: 133
PII: 20170255

Researcher Affiliations

Wu, Yabin
  • Institute for Biomechanics, ETH Zurich, Zurich, Switzerland wu.yabin@hest.ethz.ch.
Stoddart, Martin J
  • AO Research Institute, Davos, Switzerland.
  • Institute for Science and Technology in Medicine, Keele University, Stoke-on-Trent, UK.
Wuertz-Kozak, Karin
  • Institute for Biomechanics, ETH Zurich, Zurich, Switzerland.
  • Department of Health Sciences, University of Potsdam, Potsdam, Germany.
  • Schön Klinik München Harlaching, Munich, Germany.
  • Paracelsus Private Medical University, Salzburg, Austria.
Grad, Sibylle
  • AO Research Institute, Davos, Switzerland.
Alini, Mauro
  • AO Research Institute, Davos, Switzerland.
Ferguson, Stephen J
  • Institute for Biomechanics, ETH Zurich, Zurich, Switzerland.

MeSH Terms

  • Animals
  • Biomechanical Phenomena / physiology
  • Cartilage / cytology
  • Cartilage / metabolism
  • Chondrocytes / cytology
  • Chondrocytes / metabolism
  • Horses
  • Hyaluronic Acid / pharmacology
  • Joints / cytology
  • Joints / metabolism
  • Models, Biological
  • Synovial Fluid / metabolism
  • Weight-Bearing / physiology

Conflict of Interest Statement

We declare we have no competing interests.

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Citations

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