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Cells2021; 10(4); 859; doi: 10.3390/cells10040859

Hyaluronic Acid as Macromolecular Crowder in Equine Adipose-Derived Stem Cell Cultures.

Abstract: The use of macromolecular crowding in the development of extracellular matrix-rich cell-assembled tissue equivalents is continuously gaining pace in regenerative engineering. Despite the significant advancements in the field, the optimal macromolecular crowder still remains elusive. Herein, the physicochemical properties of different concentrations of different molecular weights hyaluronic acid (HA) and their influence on equine adipose-derived stem cell cultures were assessed. Within the different concentrations and molecular weight HAs, the 10 mg/mL 100 kDa and 500 kDa HAs exhibited the highest negative charge and hydrodynamic radius, and the 10 mg/mL 100 kDa HA exhibited the lowest polydispersity index and the highest % fraction volume occupancy. Although HA had the potential to act as a macromolecular crowding agent, it did not outperform carrageenan and Ficoll, the most widely used macromolecular crowding molecules, in enhanced and accelerated collagen I, collagen III and collagen IV deposition.
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Publication Date: 2021-04-09 PubMed ID: 33918830PubMed Central: PMC8070604DOI: 10.3390/cells10040859Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the suitability of different configurations of hyaluronic acid (HA) as a macromolecular crowder in the culture of equine adipose-derived stem cells, comparing performance against commonly used crowding agents, carrageenan and Ficoll.

Objective of the Research

  • The study aims to explore and identify the optimal macromolecular crowder for use in the development of extracellular matrix-rich cell-assembled tissue equivalents, a crucial component in regenerative engineering. More specifically, the research investigates the efficacy of hyaluronic acid (HA) in different concentrations and molecular weights as a potential candidate.

Methodology

  • The physicochemical properties of HA in different concentrations and weights were evaluated for their influence on equine adipose-derived stem cell cultures. Key metrics assessed include negative charge, hydrodynamic radius, polydispersity index, and % fraction volume occupancy.
  • Specifically, the 10 mg/mL 100kDa and 500kDa configurations of HA were examined for their negative charge and hydrodynamic radius. The 10 mg/mL 100kDa HA was also evaluated for its polydispersity index and volume occupancy.

Findings

  • The findings revealed that while HA demonstrated potential as a macromolecular crowding agent, it did not exceed the performance of carrageenan and Ficoll, the most popular macromolecular crowding agents. This was validated by assessing the accelerated deposition of collagen types I, III, and IV.
  • The study found 10 mg/mL 100kDa and 500kDa HA having the highest negative charge and hydrodynamic radius. The 10 mg/mL 100kDa HA configuration exhibited the lowest polydispersity index and the highest % fraction volume occupancy.

Implications

  • This research contributes insights towards finding the best macromolecular crowder for use in extracellular matrix-rich cell-assembled tissue equivalents. It helps in understanding how HA, carrageenan and Ficoll influence the growth and development of stem cell cultures, key to enhancing regenerative medicine techniques.
  • While HA demonstrated key attributes for a crowding agent, the fact that it was outperformed by carrageenan and Ficoll suggests more research is needed to optimize its use or identify alternative compounds.

Cite This Article

APA
Garnica-Galvez S, Korntner SH, Skoufos I, Tzora A, Diakakis N, Prassinos N, Zeugolis DI. (2021). Hyaluronic Acid as Macromolecular Crowder in Equine Adipose-Derived Stem Cell Cultures. Cells, 10(4), 859. https://doi.org/10.3390/cells10040859

Publication

Cells
ISSN: 2073-4409
NlmUniqueID: 101600052
Country: Switzerland
Language: English
Volume: 10
Issue: 4
PII: 859

Researcher Affiliations

Garnica-Galvez, Sergio
  • Laboratory of Animal Science, Nutrition and Biotechnology, Department of Agriculture, University of Ioannina, 47100 Arta, Greece.
  • School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), H92 W2TY Galway, Ireland.
Korntner, Stefanie H
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), H92 W2TY Galway, Ireland.
  • Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), H92 W2TY Galway, Ireland.
Skoufos, Ioannis
  • Laboratory of Animal Science, Nutrition and Biotechnology, Department of Agriculture, University of Ioannina, 47100 Arta, Greece.
Tzora, Athina
  • Laboratory of Animal Science, Nutrition and Biotechnology, Department of Agriculture, University of Ioannina, 47100 Arta, Greece.
Diakakis, Nikolaos
  • School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Prassinos, Nikitas
  • School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Zeugolis, Dimitrios I
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), H92 W2TY Galway, Ireland.
  • Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), H92 W2TY Galway, Ireland.
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Faculty of Biomedical Sciences, Università della Svizzera Italiana (USI), 6904 Lugano, Switzerland.
  • Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), School of Mechanical and Materials Engineering, University College Dublin (UCD), D04 V1W8 Dublin, Ireland.

MeSH Terms

  • Adipose Tissue / cytology
  • Animals
  • Cell Proliferation
  • Cell Shape
  • Cell Survival
  • Cells, Cultured
  • Dynamic Light Scattering
  • Horses
  • Hyaluronic Acid / metabolism
  • Macromolecular Substances / metabolism
  • Solubility
  • Stem Cells / cytology

Grant Funding

  • 866126 / European Research Council
  • 676338 / H2020 Marie Sku0142odowska-Curie Actions
  • 15/CDA/3629; 19/FFP/6982; 13/RC/2073_2. / Science Foundation Ireland

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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