Hybridization of bovine papilloma virus type 1 and type 2 DNA to DNA from virus-induced hamster tumors and naturally occurring equine tumors.
Abstract: DNAs from bovine papilloma virus(BPV)-induced hamster tumors and from equine connective tissue tumors of unknown etiology were examined for BPV DNA sequences by molecular hybridization. DNA from two distinct classes of BPV (type 1 and type 2) was labeled in vitro and used as probes. Analysis of DNA-DNA reassociation kinetics indicated that both virus types were capable of tumor induction in the hamster. DNA isolated from 6 of 7 equine tumors accelerated the reassociation of the BPV DNA probes. BPV type 1 or type 2 DNA hybridized extensively to DNA from 3 tumors, while 3 other tumors contained DNA sequences to which only a portion of the probes hybridized. Partial hybridization of probe DNAs to tumor DNA suggested the possible existence of a third BPV class.
Publication Date: 1979-01-01 PubMed ID: 218902DOI: 10.1159/000149038Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This study investigates the role of bovine papilloma virus (BPV) in causing tumors in hamsters and horses. Researchers found that both BPV type 1 and type 2 can cause tumors in hamsters and that DNA from both these virus types were also found in 6 out of 7 horse tumors. They also suggest a possible third type of BPV.
Research Methodology
- The researchers utilized the method of molecular hybridization to examine the presence of BPV DNA sequences in DNA samples taken from hamster tumors caused by BPV, and from equine connective tissue tumors of unknown cause.
- The DNA from both BPV types (type 1 and type 2) was labelled in the lab and used as probes to detect the presence of the same DNA in the analyzed tumor samples.
- A detailed analysis of the DNA-DNA reassociation kinetics was performed to discern whether both virus types were capable of inducing tumor formation in hamsters.
Results and Discussion
- The study found that both BPV types were indeed capable of causing tumor induction in the hamster.
- When analyzing the equine tumor DNA samples, the probes of BPV DNA sequences accelerated the reassociation process in 6 out of 7 studied tumors.
- The DNA analysis for 3 of the tumors showed extensive hybridization with both BPV type 1 and type 2 DNA.
- In the other three tumors, only parts of the BPV DNA probes were able to hybridize with the tumor DNA. This partial hybridization result led to the suggestion that there may exist a third type of BPV.
Conclusion
- This research presents important findings on the correlation between BPV and the induction of tumors in hamsters and horses. The results show that the DNA sequences of both BPV type 1 and type 2 can be found in the examined tumors.
- The study also deduces that there might be a third class of BPV, based on partial hybridization seen in three of the equine tumors. However, this hypothesis would need further research for confirmation.
Cite This Article
APA
Lancaster WD, Theilen GH, Olson C.
(1979).
Hybridization of bovine papilloma virus type 1 and type 2 DNA to DNA from virus-induced hamster tumors and naturally occurring equine tumors.
Intervirology, 11(4), 227-233.
https://doi.org/10.1159/000149038 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Base Sequence
- Bovine papillomavirus 1 / analysis
- Cricetinae
- DNA, Neoplasm / analysis
- DNA, Viral / analysis
- Horse Diseases / metabolism
- Horses
- Neoplasms / analysis
- Neoplasms / veterinary
- Neoplasms, Experimental / analysis
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Papillomaviridae / analysis
Citations
This article has been cited 10 times.- Russo V, Roperto F, De Biase D, Cerino P, Urraro C, Munday JS, Roperto S. Bovine Papillomavirus Type 2 Infection Associated with Papillomatosis of the Amniotic Membrane in Water Buffaloes (Bubalus bubalis).. Pathogens 2020 Apr 4;9(4).
- Roperto S, Russo V, Corrado F, De Falco F, Munday JS, Roperto F. Oral fibropapillomatosis and epidermal hyperplasia of the lip in newborn lambs associated with bovine Deltapapillomavirus.. Sci Rep 2018 Sep 6;8(1):13310.
- Christensen ND, Budgeon LR, Cladel NM, Hu J. Recent advances in preclinical model systems for papillomaviruses.. Virus Res 2017 Mar 2;231:108-118.
- Altamura G, Corteggio A, Nasir L, Yuan ZQ, Roperto F, Borzacchiello G. Analysis of activated platelet-derived growth factor β receptor and Ras-MAP kinase pathway in equine sarcoid fibroblasts.. Biomed Res Int 2013;2013:283985.
- Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids.. J Clin Microbiol 2013 Jul;51(7):2167-71.
- Moar MH, Campo MS, Laird HM, Jarrett WF. Unintegrated viral DNA sequences in a hamster tumor induced by bovine papilloma virus.. J Virol 1981 Sep;39(3):945-9.
- Amtmann E, Müller H, Sauer G. Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA.. J Virol 1980 Sep;35(3):962-4.
- Engel LW, Heilman CA, Howley PM. Transcriptional organization of bovine papillomavirus type 1.. J Virol 1983 Sep;47(3):516-28.
- Angelos JA, Marti E, Lazary S, Carmichael LE. Characterization of BPV-like DNA in equine sarcoids.. Arch Virol 1991;119(1-2):95-109.
- Lancaster WD. Physical maps of bovine papillomavirus type 1 and type 2 genomes.. J Virol 1979 Nov;32(2):684-7.
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