Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells without need for recombinant proteins.
Abstract: In vivo tissue regeneration depends on migration of stem cells into injured areas, their differentiation into specific cell types, and their interaction with other cells that are necessary to generate new tissue. Human mesenchymal stem cells, a subset of bone marrow stromal cells (BMSCs), can migrate and differentiate into osteoblasts in bone tissue. This can be facilitated by recombinant growth factors and cytokines. In many animal species, the availability of genomic sequences, recombinant proteins, and/or antibodies is limited so that new approaches are needed to generate resources that facilitate migration of stem cells into tissue defect areas. Here we used bone marrow stromal cells of human, ovine, equine, and canine origin to generate hypoxia-conditioned media (HCM) in order to attract BMSCs of the respective species in migration assays. Results: We show that HCM contain attractors even more potent than vascular endothelial growth factor and can therefore be used in many animal species without the need for purified proteins. Conclusions: Generation of HCM is easy and cheap compared to preparation and purification of protein fractions and/or recombinant proteins. Hence, HCM could be applied in large animals (e.g. sheep, horse, dogs) for attraction of BMSCs into tissue defects caused by tumor resection or trauma.
Publication Date: 2014-03-04 PubMed ID: 24593914PubMed Central: PMC3974005DOI: 10.1186/1746-6148-10-56Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focused on the use of hypoxia-conditioned media to attract bone marrow stromal cells from various species, eliminating the need for expensive recombinant proteins typically used to stimulate the migration of these cells. By using this method, the researchers suggest a cost-effective alternative for tissue regeneration, particularly in larger animals.
Objective of the Research
- The research aimed to develop a new method for stimulating the migration of bone marrow stromal cells (a subset of mesenchymal stem cells) into damaged tissue areas for regeneration.
- The study sought to identify an alternative to the use of recombinant growth factors or cytokines, which are often expensive and limited in availability for many animal species.
Hypoxia-Conditioned Media (HCM)
- The researchers generated Hypoxia-Conditioned Media (HCM) using bone marrow stromal cells from humans, sheep, horses, and dogs.
- HCM was found to contain attractors more potent than the vascular endothelial growth factor, a common recombinant protein used to stimulate cell migration.
- This suggests that HCM can be applied across many animal species to attract bone marrow stromal cells to tissue defects without the need for expensive recombinant proteins.
Implications of the Research
- The development of HCM provides a more cost-effective and universally applicable alternative to the use of recombinant proteins for stem cell migration.
- This new method could be particularly useful for tissue regeneration in large animals, as it circumvents limitations related to the purification and preparation of protein fractions or recombinant proteins.
- As such, HCM has potential applications in the healing of tissue defects caused by trauma or tumor resection.
Conclusions
- The study demonstrated that Hypoxia-Conditioned Media is an efficient and cost-effective alternative to recombinant proteins for stimulating the migration of bone marrow stromal cells into damaged tissues for regeneration.
- This method holds promise for expanding treatment options for large animals and could lead to advancements in veterinary medicine, especially in the field of tissue regeneration.
Cite This Article
APA
Gabrielyan A, Knaak S, Gelinsky M, Arnhold S, Rösen-Wolff A.
(2014).
Hypoxia-conditioned media allows species-specific attraction of bone marrow stromal cells without need for recombinant proteins.
BMC Vet Res, 10, 56.
https://doi.org/10.1186/1746-6148-10-56 Publication
Researcher Affiliations
- Department of Paediatrics, University Clinic Carl Gustav Carus, TU Dresden, Dresden, Germany. Angela.Roesen-Wolff@uniklinikum-dresden.de.
MeSH Terms
- Amino Acid Sequence
- Animals
- Cell Movement
- Culture Media, Conditioned
- Dogs
- HMGB1 Protein / genetics
- HMGB1 Protein / metabolism
- Horses
- Humans
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / physiology
- Oxygen
- Receptor for Advanced Glycation End Products
- Receptors, Immunologic / genetics
- Receptors, Immunologic / metabolism
- Sheep
- Species Specificity
- Vascular Endothelial Growth Factor A / genetics
- Vascular Endothelial Growth Factor A / metabolism
References
This article includes 11 references
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