Hysteroscopic insemination of mares with low numbers of nonsorted or flow sorted spermatozoa.
Abstract: The objectives of this study were 1) to compare pregnancy rates resulting from 2 methods of insemination using low sperm numbers and 2) to compare pregnancy rates resulting from hysteroscopic insemination of 5 x 106 nonsorted and 5 x 106 spermatozoa sorted for X- and Y-chromosome-bearing populations (flow sorted). Semen was collected with an artificial vagina from 2 stallions of known acceptable fertility. Oestrus was synchronised (June to July) in 40 mares, age 3-10 years, by administering 10 ml altrenogest orally for 10 consecutive days, followed by 250 microg cloprostenol i.m. on Day 11. All mares were given 3000 iu hCG i.v. at the time of insemination to induce ovulation. Mares were assigned randomly to 1 of 3 treatment groups: mares in Treatment 1 (n = 10) were inseminated with 5 x 10(6) spermatozoa deposited deep into the uterine horn with the aid of ultrasonography. Mares in Treatment 2 (n = 10) were inseminated with 5 x 10(6) spermatozoa deposited onto the uterotubal junction papilla via hysteroscopic insemination. Mares in Treatment 3 (n = 20) were inseminated using the hysteroscopic technique with 5 x 10(6) flow sorted spermatozoa. Spermatozoa were stained with Hoechst 33342 and sorted into X- and Y-chromosome-bearing populations based on DNA content using an SX MoFlo sperm sorter. Pregnancy was determined ultrasonographically at 16 days postovulation. Hysteroscopic insemination resulted in more pregnancies (5/10 = 50%) than did the ultrasound-guided technique (0/10 = 0%; P<0.05) when nonsorted sperm were inseminated. Pregnancy rates were not significantly lower (P>0.05) when hysteroscopic insemination was used for sorted (5/20 = 25%) and nonsorted spermatozoa (5/10 = 50%). Therefore, hysteroscopic insemination of low numbers of flow sorted stallion spermatozoa resulted in reasonable pregnancy rates.
Publication Date: 2002-03-22 PubMed ID: 11905434DOI: 10.2746/042516402776767178Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research study aimed to evaluate the effectiveness of two insemination methods that use low sperm count in inducing pregnancy in mares and to compare results using both nonsorted and flow sorted spermatozoa. The study found that hysteroscopic insemination resulted in higher pregnancy rates compared to the ultrasound-guided technique when nonsorted sperm were utilized, and that the rates were similar whether the sperm used was sorted or not.
Research Methodology
- The researchers collected semen from two stallions with known fertility.
- Forty mares, aged between 3 to 10 years, were selected. Their estrus was synchronized by administering oral altrenogest for 10 consecutive days, followed by cloprostenol intramuscularly on the 11th day.
- All mares were given hCG intravenously at the time of insemination to induce ovulation.
- The mares were then divided into three treatment groups. The first group (n = 10) was inseminated with 5 x 106 spermatozoa using an ultrasonography-guided technique. The second group (n = 10) was inseminated with the same amount of spermatozoa using a hysteroscopic insemination technique. The third group (n = 20) was also inseminated using hysteroscopic insemination but with the spermatozoa being flow sorted.
- The flow sorting process involved staining the spermatozoa with Hoechst 33342 and sorting them based on their DNA content.
Results and Findings
- Pregnancy was confirmed ultrasonographically 16 days after ovulation.
- The research found that hysteroscopic insemination was more successful in leading to pregnancy (50%) compared to the ultrasound-guided technique (0%).
- Hysteroscopic insemination had similar success rates whether using sorted (25%) or nonsorted spermatozoa (50%).
Conclusions
- The study concludes that hysteroscopic insemination is a more effective method than ultrasound-guided techniques when low numbers of spermatozoa are used.
- The results showed reasonable pregnancy rates when small amounts of flow sorted stallion spermatozoa are used, comparing favorably to the rate when using nonsorted spermatozoa.
Cite This Article
APA
Lindsey AC, Morris LH, Allen WR, Schenk JL, Squires EL, Bruemmer JE.
(2002).
Hysteroscopic insemination of mares with low numbers of nonsorted or flow sorted spermatozoa.
Equine Vet J, 34(2), 128-132.
https://doi.org/10.2746/042516402776767178 Publication
Researcher Affiliations
- Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523, USA.
MeSH Terms
- Animals
- Female
- Flow Cytometry / methods
- Flow Cytometry / veterinary
- Horses / physiology
- Hysteroscopy / veterinary
- Insemination, Artificial / methods
- Insemination, Artificial / veterinary
- Male
- Pregnancy
- Pregnancy Rate
- Random Allocation
- Sex Chromosomes
- Sperm Count / veterinary
Citations
This article has been cited 2 times.- Umehara T, Tsujita N, Zhu Z, Ikedo M, Shimada M. A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos. Nat Protoc 2020 Aug;15(8):2645-2667.
- Nakao S, Takeo T, Watanabe H, Kondoh G, Nakagata N. Successful selection of mouse sperm with high viability and fertility using microfluidics chip cell sorter. Sci Rep 2020 Jun 1;10(1):8862.
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