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Home / Equine Research Bank / Virus Genes / Identification and comparative sequence analysis of a gene i...
Virus genes1991; 5(4); 313-325; doi: 10.1007/BF00271530

Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene.

Abstract: A homologue of the herpes simplex virus (HSV) glycoprotein D gene has been identified in the genome of equine herpesvirus-1 (EHV-1, equine abortion virus). An open reading frame in the middle of the short unique (US) region is capable of encoding a polypeptide of 402 amino acids that has 26% and 20% of its residues matching pseudorabies virus (PRV) gp50 and HSV-1 gD, respectively. Despite this low level of similarity, the positional identity of six cysteine residues and certain motifs, and the location of the EHV-1 gene, clearly define the EHV-1 polypeptide as one of a family of "gD-like" proteins. Two transcripts of 3.3-3.6 kb and 5.4-5.9 kb were identified, consistent with coterminal mRNAs for the EHV-1 gD gene and the adjacent upstream gene, respectively. Partial sequencing of other regions in US also revealed EHV-1 homologues of HSV-1 gE and gI genes, and a possible equivalent gene to PRV gX. By analogy with the ability of HSV-1 gD and PRV gp50 to induce strong anti-viral immune responses, the EHV-1 gD gene product is expected to be an excellent candidate for development as a vaccine antigen.
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Publication Date: 1991-10-01 PubMed ID: 1665613DOI: 10.1007/BF00271530Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research worked on identifying a gene in equine herpesvirus-1 (a virus known to cause abortions in horses) that is similar to the herpes simplex virus glycoprotein D gene that is well known. The research shows that this identified gene can produce a protein that matches elements of similar proteins in two other viruses. Results from the study imply that this identified gene’s product could have a promising role in the development of better vaccines.

Objective of the study

  • The primary objective of this research was to identify and comprehensively analyze a gene found in the equine herpesvirus-1 (EHV-1) genome. The special focus was given to a gene that indicated a similarity or homology to the herpes simplex virus (HSV) glycoprotein D gene.

Findings of the study

  • The researchers have recognized an open reading frame in the short unique (US) region of the EHV-1 genome. This reading frame is capable of producing a polypeptide that contains about 26% and 20% of its residues matching with pseudorabies virus (PRV) gp50 and HSV-1 gD respectively.
  • Despite the observed low level of similarity between the elements, the location and features of the EHV-1 gene and certain integral components clearly define the produced protein as part of a “gD-like” family of proteins.
  • Moreover, two specific transcripts of determinable size were identified, which are assumed to be coterminal mRNAs for the EHV-1 gD gene and its adjacent upstream gene.
  • Other areas partially sequenced in the short unique (US) region hinted at the presence of EHV-1 equivalents to HSV-1 gE and gI genes, as well as a probable equivalent to PRV gX gene.

Implications of the research

  • Drawing parallels with the capacity of HSV-1 gD and PRV gp50 to provoke strong anti-viral immune responses, the researchers suggest that the product of the EHV-1 gD gene could potentially be utilized in the development of effective vaccine antigens.
  • It also aids in gaining a deeper understanding of the genetic makeup of the EHV-1, particularly the gene structure and its correlation to the herpes simplex virus, which could prove significant in devising new therapeutic strategies.

Cite This Article

APA
Whalley M, Robertson G, Bell C, Love D, Elphinstone M, Wiley L, Craven D. (1991). Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene. Virus Genes, 5(4), 313-325. https://doi.org/10.1007/BF00271530

Publication

Virus genes
ISSN: 0920-8569
NlmUniqueID: 8803967
Country: United States
Language: English
Volume: 5
Issue: 4
Pages: 313-325

Researcher Affiliations

Whalley, M
  • School of Biological Sciences, Macquarie University, Sydney, Australia.
Robertson, G
    Bell, C
      Love, D
        Elphinstone, M
          Wiley, L
            Craven, D

              MeSH Terms

              • Amino Acid Sequence
              • Base Sequence
              • Blotting, Northern
              • Cysteine / genetics
              • Genes, Viral
              • Herpesvirus 1, Equid / genetics
              • Molecular Sequence Data
              • Open Reading Frames / genetics
              • Restriction Mapping
              • Sequence Alignment
              • Sequence Homology, Nucleic Acid
              • Simplexvirus / genetics
              • Viral Envelope Proteins / chemistry
              • Viral Envelope Proteins / genetics

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              Citations

              This article has been cited 5 times.
              1. Azab W, Osterrieder N. Glycoproteins D of equine herpesvirus type 1 (EHV-1) and EHV-4 determine cellular tropism independently of integrins. J Virol 2012 Feb;86(4):2031-44.
                doi: 10.1128/JVI.06555-11pubmed: 22171258google scholar: lookup
              2. Wellington JE, Lawrence GL, Love DN, Whalley JM. Expression and characterization of equine herpesvirus 1 glycoprotein D in mammalian cell lines. Arch Virol 1996;141(9):1785-93.
                doi: 10.1007/BF01718301pubmed: 8893800google scholar: lookup
              3. Wellington JE, Love DN, Whalley JM. Evidence for involvement of equine herpesvirus 1 glycoprotein B in cell-cell fusion. Arch Virol 1996;141(1):167-75.
                doi: 10.1007/BF01718598pubmed: 8629945google scholar: lookup
              4. Love DN, Bell CW, Pye D, Edwards S, Hayden M, Lawrence GL, Boyle D, Pye T, Whalley JM. Expression of equine herpesvirus 1 glycoprotein D by using a recombinant baculovirus. J Virol 1993 Nov;67(11):6820-3.
                doi: 10.1128/JVI.67.11.6820-6823.1993pubmed: 8411384google scholar: lookup
              5. Eberle R, Zhang M, Black DH. Gene mapping and sequence analysis of the unique short region of the simian herpesvirus SA 8 genome. Arch Virol 1993;130(3-4):391-411.
                doi: 10.1007/BF01309669pubmed: 8390827google scholar: lookup
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