Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB.
Abstract: A gene in equine herpesvirus 1 (EHV-1; equine abortion virus) equivalent to the gB glycoprotein gene of herpes simplex virus (HSV) has been identified by DNA hybridization and nucleotide sequencing. A 4.3 kbp EHV-1 PstI-ClaI sequence (0.40 to 0.43 map units) contained an open reading frame flanked by appropriate control elements and was capable of encoding a polypeptide of 980 amino acids. This had 50 to 60% identity over a 617 amino acid conserved region with the gB gene products of HSV and three other alphaherpesviruses, and 20 to 30% identity with those of human cytomegalovirus and Epstein-Barr virus. Analysis of the amino acid sequence predicts a long signal peptide, hydrophobic and hydrophilic domains and N-glycosylation sites, and has identified a probable internal proteolytic cleavage site. The EHV-1 gB open reading frame appears to be overlapped at its 5' end by 135 nucleotides of the 3' end of an upstream open reading frame the potential translation product of which has approximately 50% identity with HSV gene ICP 18.5 and VZV gene 30 products.
Publication Date: 1989-02-01 PubMed ID: 2543744DOI: 10.1099/0022-1317-70-2-383Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research identifies and sequences a gene in equine herpesvirus 1 (EHV-1) that is similar to the herpes simplex virus (HSV) gene encoding for the major envelope protein gB.
Identification of Glycoprotein-gene in Equine Herpesvirus
The researchers in this study successfully identified a gene in Equine Herpesvirus 1 (EHV-1), commonly referred to as the equine abortion virus, which is equivalent to the herpes simplex virus’s (HSV’s) gB glycoprotein gene. The gene identification was accomplished using techniques like DNA hybridization and nucleotide sequencing.
- The identified gene was located within a 4.3 Kbp EHV-1 PstI-ClaI sequence, situated between 0.40 to 0.43 map units.
- The sequence contained an open reading frame (ORF), flanked by the necessary control elements, capable of encoding a polypeptide comprising 980 amino acids.
- The encoded polypeptide showcased a 50 to 60% identity over a 617-amino acid conserved area with gB gene products of HSV and three other alphaherpesviruses.
- With gB gene products of the human cytomegalovirus and the Epstein-Barr virus, it showed 20 to 30% identity.
Analyzing the Amino Acid Sequence
Upon analyzing the amino acid sequence, the following characteristics were noted within the gB gene:
- The amino acid sequence included a long signal peptide, hydrophobic and hydrophilic domains, and N-glycosylation sites.
- A probable internal proteolytic cleavage site was identified.
Additional Open Reading Frame Findings
In addition to the significant identification of the gB gene, the researchers also noted an overlap at the 5′ end of the gB open reading frame (ORF) by 135 nucleotides from the 3′ end of an upstream ORF.
- This upstream ORF’s potential translation product showed approximately 50% identity with HSV gene ICP 18.5 and Varicella-Zoster Virus (VZV) gene 30 products.
In conclusion, this research has helped in identifying and understanding the gene in equine herpesvirus 1 analogous to the gB glycoprotein gene of herpes simplex virus, adding value to the overall study of these viruses. This type of research has immense potential in the development of new treatments and vaccines for equine herpesvirus 1 and herpes simplex virus.
Cite This Article
APA
Whalley JM, Robertson GR, Scott NA, Hudson GC, Bell CW, Woodworth LM.
(1989).
Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB.
J Gen Virol, 70 ( Pt 2), 383-394.
https://doi.org/10.1099/0022-1317-70-2-383 Publication
Researcher Affiliations
- School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
MeSH Terms
- Amino Acid Sequence
- Base Sequence
- DNA, Viral / genetics
- Genes, Viral
- Glycoproteins / genetics
- Herpesviridae / genetics
- Herpesvirus 1, Equid / genetics
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Peptides / genetics
- Plasmids
- Sequence Homology, Nucleic Acid
- Simplexvirus / genetics
- Viral Envelope Proteins / genetics
Citations
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