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Viruses2019; 11(8); 713; doi: 10.3390/v11080713

Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion.

Abstract: Papillomaviruses (PVs) have been identified in a wide range of animal species and are associated with a variety of disease syndromes including classical papillomatosis, aural plaques, and genital papillomas. In horses, 13 PVs have been described to date, falling into six genera. Using total RNA sequencing (meta-transcriptomics) we identified a novel equine papillomavirus in semen taken from a thoroughbred stallion suffering a genital lesion, which was confirmed by nested RT-PCR. We designate this novel virus (EcPV9). The complete 7656 bp genome of EcPV9 exhibited similar characteristics to those of other horse papillomaviruses. Phylogenetic analysis based on concatenated E1-E2-L2-L1 amino acid sequences revealed that EcPV9 clustered with EcPV2, EcPV4, and EcPV5, although was distinct enough to represent a new viral species within the genus (69.35%, 59.25%, and 58.00% nucleotide similarity to EcPV2, EcPV4, and EcPV5, respectively). In sum, we demonstrate the presence of a novel equine papillomavirus for which more detailed studies of disease association are merited.
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Publication Date: 2019-08-04 PubMed ID: 31382657PubMed Central: PMC6723834DOI: 10.3390/v11080713Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper outlines the discovery of a new papillomavirus in horses. The virus, designated as EcPV9, was identified in the semen of a thoroughbred stallion suffering from a genital lesion.

Identification of the Novel Equine Papillomavirus

  • The researchers identified the novel equine papillomavirus (EcPV9) in a thoroughbred stallion with a genital lesion using total RNA sequencing, a technique used to identify changes in gene expression and genetic coding.
  • The presence of the virus was confirmed with additional testing using nested reverse transcriptase polymerase chain reaction (RT-PCR), a method used to amplify small amounts of RNA to make it easier to detect and analyze.

Genome Analysis

  • The full genome of the EcPV9 was 7656 base pairs long and showed similar characteristics to other known equine papillomaviruses. This helped to establish the virus as part of the same viral family.
  • Phylogenetic analysis, which shows the evolutionary relationships between different species, was performed on the E1-E2-L2-L1 amino acid sequences of the virus. This was done to understand the relationship of EcPV9 to previously known papillomaviruses in horses.

Positioning within the Genus

  • Upon analysis, it was found that EcPV9 grouped closely with EcPV2, EcPV4, and EcPV5, which are other papillomaviruses found in horses. However, its unique characteristics allowed it to be classified as a new viral species within the same genus.
  • The similarities at the nucleotide level to EcPV2, EcPV4, and EcPV5 were 69.35%, 59.25%, and 58.00% respectively, reinforcing its identity as a separate viral species.

Future Studies

  • The researchers suggest that further detailed studies are needed to understand the disease association of this new equine papillomavirus. There is a need to understand the clinical implications and potential health risk the virus may pose to horses and to potentially develop any necessary preventive or treatment strategies.

Cite This Article

APA
Li CX, Chang WS, Mitsakos K, Rodger J, Holmes EC, Hudson BJ. (2019). Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion. Viruses, 11(8), 713. https://doi.org/10.3390/v11080713

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 11
Issue: 8
PII: 713

Researcher Affiliations

Li, Ci-Xiu
  • Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life & Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.
Chang, Wei-Shan
  • Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life & Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.
Mitsakos, Katerina
  • Royal North Shore Hospital, Clinical Microbiology & Infectious Diseases, Reserve Road, St Leonards, NSW 2065, Australia.
Rodger, James
  • Vets & Veterinary Surgeons, Jerry Plains Veterinary Hospital, 10 Pagan Street, Jerry Plains, NSW 2330, Australia.
Holmes, Edward C
  • Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life & Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia. edward.holmes@sydney.edu.au.
Hudson, Bernard J
  • Royal North Shore Hospital, Clinical Microbiology & Infectious Diseases, Reserve Road, St Leonards, NSW 2065, Australia.

MeSH Terms

  • Animals
  • Breeding
  • DNA, Viral / genetics
  • Evolution, Molecular
  • Genome, Viral / genetics
  • Horse Diseases / virology
  • Horses / virology
  • Male
  • Papillomaviridae / classification
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / veterinary
  • Papillomavirus Infections / virology
  • Penis / pathology
  • Penis / virology
  • Phylogeny
  • Semen / virology
  • Species Specificity
  • Viral Proteins / genetics

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 20 references
  1. Nasir L, Brandt S. Papillomavirus associated diseases of the horse.. Vet Microbiol 2013 Nov 29;167(1-2):159-67.
    doi: 10.1016/j.vetmic.2013.08.003pubmed: 24016387google scholar: lookup
  2. Lunardi M, de Alcântara BK, Otonel RA, Rodrigues WB, Alfieri AF, Alfieri AA. Bovine papillomavirus type 13 DNA in equine sarcoids.. J Clin Microbiol 2013 Jul;51(7):2167-71.
    doi: 10.1128/JCM.00371-13pmc: PMC3697707pubmed: 23637294google scholar: lookup
  3. Torres SM, Koch SN. Papillomavirus-associated diseases.. Vet Clin North Am Equine Pract 2013 Dec;29(3):643-55.
    doi: 10.1016/j.cveq.2013.08.003pubmed: 24267681google scholar: lookup
  4. Sykora S, Brandt S. Papillomavirus infection and squamous cell carcinoma in horses.. Vet J 2017 May;223:48-54.
    doi: 10.1016/j.tvjl.2017.05.007pubmed: 28671071google scholar: lookup
  5. Lange CE, Tobler K, Ackermann M, Favrot C. Identification of two novel equine papillomavirus sequences suggests three genera in one cluster.. Vet Microbiol 2011 Apr 21;149(1-2):85-90.
    doi: 10.1016/j.vetmic.2010.10.019pubmed: 21109367google scholar: lookup
  6. Lange CE, Vetsch E, Ackermann M, Favrot C, Tobler K. Four novel papillomavirus sequences support a broad diversity among equine papillomaviruses.. J Gen Virol 2013 Jun;94(Pt 6):1365-1372.
    doi: 10.1099/vir.0.052092-0pubmed: 23486670google scholar: lookup
  7. Linder KE, Bizikova P, Luff J, Zhou D, Yuan H, Breuhaus B, Nelson E, Mackay R. Generalized papillomatosis in three horses associated with a novel equine papillomavirus (EcPV8).. Vet Dermatol 2018 Feb;29(1):72-e30.
    doi: 10.1111/vde.12481pubmed: 28833761google scholar: lookup
  8. Scase T, Brandt S, Kainzbauer C, Sykora S, Bijmholt S, Hughes K, Sharpe S, Foote A. Equus caballus papillomavirus-2 (EcPV-2): an infectious cause for equine genital cancer?. Equine Vet J 2010 Nov;42(8):738-45.
    doi: 10.1111/j.2042-3306.2010.00311.xpubmed: 21039805google scholar: lookup
  9. Lecis R, Tore G, Scagliarini A, Antuofermo E, Dedola C, Cacciotto C, Dore GM, Coradduzza E, Gallina L, Battilani M, Anfossi AG, Muzzeddu M, Chessa B, Pittau M, Alberti A. Equus asinus papillomavirus (EaPV1) provides new insights into equine papillomavirus diversity.. Vet Microbiol 2014 Jun 4;170(3-4):213-23.
    doi: 10.1016/j.vetmic.2014.02.016pubmed: 24636161google scholar: lookup
  10. Buchfink B, Xie C, Huson DH. Fast and sensitive protein alignment using DIAMOND.. Nat Methods 2015 Jan;12(1):59-60.
    doi: 10.1038/nmeth.3176pubmed: 25402007google scholar: lookup
  11. Li D, Luo R, Liu CM, Leung CM, Ting HF, Sadakane K, Yamashita H, Lam TW. MEGAHIT v1.0: A fast and scalable metagenome assembler driven by advanced methodologies and community practices.. Methods 2016 Jun 1;102:3-11.
    doi: 10.1016/j.ymeth.2016.02.020pubmed: 27012178google scholar: lookup
  12. Li D, Liu CM, Luo R, Sadakane K, Lam TW. MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph.. Bioinformatics 2015 May 15;31(10):1674-6.
    doi: 10.1093/bioinformatics/btv033pubmed: 25609793google scholar: lookup
  13. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.. Bioinformatics 2012 Jun 15;28(12):1647-9.
    doi: 10.1093/bioinformatics/bts199pmc: PMC3371832pubmed: 22543367google scholar: lookup
  14. Peters-Kennedy J, Lange CE, Rine SL, Hackett RP. Equus caballus papillomavirus 8 (EcPV8) associated with multiple viral plaques, viral papillomas, and squamous cell carcinoma in a horse.. Equine Vet J 2019 Jul;51(4):470-474.
    doi: 10.1111/evj.13046pubmed: 30456819google scholar: lookup
  15. Bernard HU, Burk RD, Chen Z, van Doorslaer K, zur Hausen H, de Villiers EM. Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendments.. Virology 2010 May 25;401(1):70-9.
    pmc: PMC3400342pubmed: 20206957doi: 10.1016/j.virol.2010.02.002google scholar: lookup
  16. Gardiol D, Kühne C, Glaunsinger B, Lee SS, Javier R, Banks L. Oncogenic human papillomavirus E6 proteins target the discs large tumour suppressor for proteasome-mediated degradation.. Oncogene 1999 Sep 30;18(40):5487-96.
    doi: 10.1038/sj.onc.1202920pubmed: 10523825google scholar: lookup
  17. Wise-Draper TM, Wells SI. Papillomavirus E6 and E7 proteins and their cellular targets.. Front Biosci 2008 Jan 1;13:1003-17.
    doi: 10.2741/2739pubmed: 17981607google scholar: lookup
  18. Narechania A, Terai M, Chen Z, DeSalle R, Burk RD. Lack of the canonical pRB-binding domain in the E7 ORF of artiodactyl papillomaviruses is associated with the development of fibropapillomas.. J Gen Virol 2004 May;85(Pt 5):1243-1250.
    doi: 10.1099/vir.0.19765-0pubmed: 15105541google scholar: lookup
  19. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability.. Mol Biol Evol 2013 Apr;30(4):772-80.
    doi: 10.1093/molbev/mst010pmc: PMC3603318pubmed: 23329690google scholar: lookup
  20. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood.. Syst Biol 2003 Oct;52(5):696-704.
    doi: 10.1080/10635150390235520pubmed: 14530136google scholar: lookup

Citations

This article has been cited 11 times.
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