Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G.
Abstract: Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNPVYSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNPVYSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.
Publication Date: 2005-01-12 PubMed ID: 15642995PubMed Central: PMC540206DOI: 10.1128/CDLI.12.1.122-124.2005Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigated a new B-cell epitope found in the type-specific region of equine herpesvirus 4 (EHV-4), which may serve as an antigen for diagnostic purposes.
Background and Objectives
- The researchers had previously identified a 12-mer B-cell epitope in the EHV-1 virus and used it as an antigen for diagnostic purposes. In this study, they extended their investigation to EHV-4, a closely related virus, to identify similar epitopes.
- The purpose of the study was to determine heterogeneity in different strains of EHV-4 and identify B-cell epitopes that could be useful in diagnosing infections.
Methodology
- The researchers compared the type-specific region of EHV-4 across various Japanese isolates of the virus.
- They identified two repeat sequences in the EHV-4 NS80567 strain—one contained a B-cell epitope similar to the one found in EHV-1, and the other contained a slightly different 11-mer, MKNNPVYSESL, which indicated variation in amino acid composition.
Findings
- The researchers found that the 11-mer peptide specifically reacted with sera from horses naturally infected with EHV-4, suggesting its potential as a diagnostic antigen.
- Interestingly, this epitope did not react with sera from horses experimentally infected with a different EHV-4 strain (TH20p), indicating type-specificity of this newly identified B-cell epitope.
Conclusion
- The discovery of this 11-mer peptide could potentially serve as an additional B-cell epitope for EHV-4, expanding the diagnostic capabilities for this virus. However, further studies will be needed to validate its usefulness across different geographic populations and EHV-4 strains.
Cite This Article
APA
Maeda K, Mizukoshi F, Hamano M, Kai K, Kondo T, Matsumura T.
(2005).
Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G.
Clin Diagn Lab Immunol, 12(1), 122-124.
https://doi.org/10.1128/CDLI.12.1.122-124.2005 Publication
Researcher Affiliations
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. kmaeda@yamaguchi-u.ac.jp
MeSH Terms
- Amino Acid Sequence
- Animals
- Enzyme-Linked Immunosorbent Assay
- Epitopes, B-Lymphocyte / immunology
- Herpesviridae Infections / diagnosis
- Herpesviridae Infections / immunology
- Herpesviridae Infections / veterinary
- Herpesvirus 4, Equid / immunology
- Molecular Sequence Data
- Polymerase Chain Reaction
- Viral Envelope Proteins / genetics
- Viral Envelope Proteins / immunology
References
This article includes 9 references
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Citations
This article has been cited 2 times.- Bannai H, Nemoto M, Tsujimura K, Yamanaka T, Maeda K, Kondo T. Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen.. J Vet Med Sci 2016 Feb;78(2):309-11.
- Liu X, Han Z, Shao Y, Yu D, Li H, Wang Y, Kong X, Liu S. Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus.. Virol J 2010 Sep 13;7:223.
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