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Home / Equine Research Bank / Folia Microbiol (Praha) / Identification of Arcanobacterium haemolyticum isolated from...
Folia microbiologica2011; 55(6); 666-668; doi: 10.1007/s12223-010-0108-4

Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse.

Abstract: An Arcanobacterium haemolyticum strain isolated from a postcastrational lesion of a horse was identified phenotypically and genotypically. The latter was performed by sequencing the 16S-23S rDNA intergenic spacer region (ISR), by amplification of the gene encoding A. haemolyticum phospholipase D, by amplification of A. haemolyticum specific parts of ISR-23S rDNA and by amplification of the newly described CAMP factor family protein encoding gene of A. haemolyticum. This indicates (as described previously for seven additional A. haemolyticum strains; Hassan et al. 2009) that A. haemolyticum seems to occur also in infections of horses.
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Publication Date: 2011-01-21 PubMed ID: 21253917DOI: 10.1007/s12223-010-0108-4Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article reports on the identification of the Arcanobacterium haemolyticum bacterium in a horse that had complications after castration. The bacterium was identified using both physical and genetic methods, demonstrating that A. haemolyticum could be involved in infections in horses.

Identification Method

  • The researchers used a number of methods to identify the Arcanobacterium haemolyticum strain in the horse. This was intended to provide a thorough and accurate identification of the bacterium.
  • The bacterium was isolated from a postcastrational lesion on the horse. This is an abnormal bodily condition resulting from the horse’s castration.
  • Phenotypic identification, which focuses on observable characteristics such as shape, size, and behavior, was employed. This offers an overview of the bacterium’s physical attributes.
  • The researchers also used genotypic identification, which looks at the bacterium’s genetic makeup. This is a more detailed and precise method, as it identifies bacteria at the molecular level.

Genotypic Identification Techniques

  • The genotypic identification involved several techniques aimed at examining the bacterium’s unique genetic characteristics.
  • One technique was sequencing the 16S-23S rDNA intergenic spacer region (ISR). Sequencing is a process of determining the order of DNA bases in a genome, hence providing detailed genetic information.
  • Amplification of the gene encoding for A. haemolyticum phospholipase D, a crucial enzyme produced by the bacterium, was another method used to provide more detailed genetic information for identification.
  • The researchers also amplified A.haemolyticum-specific parts of the ISR-23S rDNA and the newly described CAMP factor family protein encoding gene of A. haemolyticum. This involves increasing the quantity of these parts of the bacterium’s DNA for further analysis and identification.

Implications of the Findings

  • This study indicates that A. haemolyticum was present in the horse’s infection, providing a new perspective on the bacterium’s infective abilities. This is of particular interest, as it supports previous findings suggesting A. haemolyticum could be involved in equine infections.
  • Such information is invaluable for veterinary medicine as it can help to inform treatment protocols and prophylactic strategies for equine post-castration complications.
  • The study also adds to the current understanding of A. haemolyticum. Understanding the bacterium’s behavior in different hosts and environments can aid in better harnessing or controlling its impact on animal health.

Cite This Article

APA
Hijazin M, Ulbegi-Mohyla H, Alber J, Lämmler C, Hassan AA, Prenger-Berninghoff E, Weiss R, Zschöck M. (2011). Identification of Arcanobacterium haemolyticum isolated from postcastrational complications of a horse. Folia Microbiol (Praha), 55(6), 666-668. https://doi.org/10.1007/s12223-010-0108-4

Publication

Folia microbiologica
ISSN: 1874-9356
NlmUniqueID: 0376757
Country: United States
Language: English
Volume: 55
Issue: 6
Pages: 666-668

Researcher Affiliations

Hijazin, M
  • Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Frankfurter Str. 107, 35392 Gießen, Germany.
Ulbegi-Mohyla, H
    Alber, J
      Lämmler, C
        Hassan, A A
          Prenger-Berninghoff, E
            Weiss, R
              Zschöck, M

                MeSH Terms

                • Actinomycetales Infections / microbiology
                • Actinomycetales Infections / veterinary
                • Animals
                • Arcanobacterium / classification
                • Arcanobacterium / genetics
                • Arcanobacterium / isolation & purification
                • Arcanobacterium / physiology
                • Bacterial Proteins / genetics
                • Castration / adverse effects
                • Castration / veterinary
                • Cluster Analysis
                • DNA, Bacterial / chemistry
                • DNA, Bacterial / genetics
                • DNA, Ribosomal Spacer / chemistry
                • DNA, Ribosomal Spacer / genetics
                • Hemolysin Proteins / genetics
                • Hemolysis
                • Horse Diseases / microbiology
                • Horses / microbiology
                • Molecular Sequence Data
                • Phospholipase D / genetics
                • Phylogeny
                • Sequence Analysis, DNA
                • Surgical Wound Infection / microbiology
                • Surgical Wound Infection / veterinary

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                Citations

                This article has been cited 2 times.
                1. Ruther HS, Phillips K, Ross D, Crawford A, Weidner MP, Sammra O, Lämmler C, McGee DJ. Smooth and Rough Biotypes of Arcanobacterium haemolyticum Can Be Genetically Distinguished at the Arcanolysin Locus. PLoS One 2015;10(9):e0137346.
                  doi: 10.1371/journal.pone.0137346pubmed: 26382754google scholar: lookup
                2. Sammra O, Friis-Møller A, Balbutskaya A, Hijazin M, Nagib S, Alber J, Lämmler C, Abdulmawjood A, Timke M, Kostrzewa M, Prenger-Berninghoff E. Phenotypic and genotypic characteristics of Arcanobacterium haemolyticum isolated from clinical samples in a Danish hospital. Folia Microbiol (Praha) 2014 Sep;59(5):369-74.
                  doi: 10.1007/s12223-014-0308-4pubmed: 24531868google scholar: lookup
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