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Identification of Five Gelatins Based on Marker Peptides from Type I Collagen by Mass Spectrum in Multiple Reaction Monitoring Mode.
Abstract: In this study, a novel pseudo-targeted peptidomics strategy, integrating the transition list generated by an in-house software (Pep-MRMer) and the retention time transfer by high-abundance ion-based retention time calibration (HAI-RT-cal), was developed to screen marker peptides of gelatins from five closely related animal species, including porcine, bovine, horse, mule, and donkey. Five marker peptides were screened from the molecular phenotypic differences of type I collagen. Furthermore, a simple and robust 10 min multiple reaction monitoring (MRM) method was established and performed well in distinguishing different gelatins, particularly in discerning horse-hide gelatin (HHG) and mule-hide gelatin (MHG) from donkey-hide gelatin (DHG). The market investigation revealed the serious adulteration of DHG. Meantime, the pseudo-targeted peptidomics could be used to screen marker peptides of other gelatin foods.
Publication Date: 2023-04-03 PubMed ID: 37010496DOI: 10.1021/acs.jafc.3c00151Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The study focuses on the development of a new method to distinguish gelatin from five different animal sources, namely pig, cow, horse, mule, and donkey using peptides from type I collagen and mass spectrometry.
Method
- The researchers devised a unique pseudo-targeted peptidomics strategy. Peptidomics is a branch of proteomics that studies peptides, which are small proteins.
- This strategy combined a transition list from an in-house software called Pep-MRMer and a high-abundance ion-based retention time calibration (HAI-RT-cal). These were used to locate marker peptides, which would help distinguish between the gelatins derived from different animal species.
Results
- Five marker peptides were identified based on the molecular phenotypic differences of type I collagen, the most abundant protein in the animal kingdom often used to make gelatin.
- The team devised a simple and efficient 10-minute multiple reaction monitoring (MRM) method, which performed well in differentiating the origin source of the gelatins, especially distinguishing horse-hide gelatin (HHG) and mule-hide gelatin (MHG) from donkey-hide gelatin (DHG).
Findings and Further Applications
- The study uncovered frequent adulteration in the market of donkey-hide gelatin (DHG). This suggests that the newly proposed method can be used to verify the authenticity of gelatin food products, contributing to food safety and preventing food fraud.
- The pseudo-targeted peptidomics strategy can be expanded to screen marker peptides for other gelatin-based foods, making it a versatile tool in identifying food composition and detecting possible adulteration.
Cite This Article
APA
Yang P, Bi Q, Li Y, Liao J, Ding Y, Huang D, Luo X, Huang Y, Yao C, Zhang J, Wei W, Li Z, Meng J, Guo D.
(2023).
Identification of Five Gelatins Based on Marker Peptides from Type I Collagen by Mass Spectrum in Multiple Reaction Monitoring Mode.
J Agric Food Chem, 71(14), 5851-5860.
https://doi.org/10.1021/acs.jafc.3c00151 Publication
Researcher Affiliations
- Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, People's Republic of China.
- Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, People's Republic of China.
- Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Research Center of TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
- University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, People's Republic of China.
MeSH Terms
- Horses
- Animals
- Cattle
- Swine
- Gelatin / chemistry
- Collagen Type I
- Peptides / chemistry
- Mass Spectrometry / methods
- Equidae
Citations
This article has been cited 1 times.- Wang K, Xu L, Liao X, Xu Z. Unveiling low-abundance ACE inhibitory peptides in not-from-concentrate orange juice processed by high-pressure processing and pasteurization. NPJ Sci Food 2025 Oct 24;9(1):209.
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