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Identification of immunoglobulin heavy-chain isotypes of specific antibodies of horse 46 group B meningococcal antiserum.
Abstract: Hyperimmune horse serum from a single animal (horse 46) immunized with group B (strain B-11) meningococcal vaccine provides a standardized, readily available diagnostic reagent used in primary isolation medium and for serogrouping of meningococci. Identification of the heavy-chain isotypes of specific anticapsular polysaccharide and anti-lipopolysaccharide isolated from horse 46 serum revealed a differential distribution in the occurrence of immunoglobulin classes. Meningococcal anticapsular antibodies of horse 46 serum were restricted predominately to the immunoglobulin M (IgM) class, with only trace amounts of IgGa present, whereas anti-lipopolysaccharide concomitantly produced showed a heterogeneity in its heavy-chain isotypes, consisting of IgM, IgGa, IgGb, moderate amounts of IgB, and a small amount of IgA.
Publication Date: 1982-02-01 PubMed ID: 6802867PubMed Central: PMC272085DOI: 10.1128/jcm.15.2.324-329.1982Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article discusses the identification of different types of immunoglobulin heavy-chain isotypes in horse 46 which was injected with group B meningococcal vaccine. It was observed that the presence of different classes of immunoglobulins varied based on the type of antibodies targeted.
Immunoglobulin Isotypes in Hyperimmune Horse Serum
- In the research, serum from a single horse, termed horse 46, was used. The horse was immunized with a group B meningococcal vaccine to produce a hyperimmune serum. This serum served as a diagnostic tool in the identification and isolation of meningococci, a bacterium responsible for meningitis and septicemia.
Antibodies and Associated Heavy-Chain Isotypes
- Two main types of antibodies isolated from the horse 46 serum were anticapsular polysaccharide and anti-lipopolysaccharide.
- The study found that anticapsular polysaccharide antibodies were predominantly related to the immunoglobulin M (IgM) class, with only trace amounts of immunoglobulin Ga (IgGa) present.
- Unlike anticapsular antibodies, anti-lipopolysaccharide antibodies showed a mixture of heavy-chain isotypes, including IgM, IgGa, immunoglobulin Gb (IgGb), moderate amounts of immunoglobulin B (IgB), and a small amount of immunoglobulin A (IgA).
Significance of the Research
- The differential occurrence of immunoglobulin classes in the hyperimmune horse serum gives new insights into understanding the immune response of horses to meningococcal infections. This knowledge may inform the development of more effective vaccines and diagnostic tests for this dangerous disease.
Cite This Article
APA
Allen PZ, Glode M, Schneerson R, Robbins JB.
(1982).
Identification of immunoglobulin heavy-chain isotypes of specific antibodies of horse 46 group B meningococcal antiserum.
J Clin Microbiol, 15(2), 324-329.
https://doi.org/10.1128/jcm.15.2.324-329.1982 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antibodies, Bacterial / isolation & purification
- Chromatography, Gel
- Horses / immunology
- Immune Sera / analysis
- Immunization
- Immunodiffusion
- Immunoglobulin Heavy Chains / isolation & purification
- Neisseria meningitidis / immunology
References
This article includes 18 references
- J Exp Med. 1967 Feb 1;125(2):249-75
- J Infect Dis. 1970 Jul-Aug;122(1):100-3
- J Immunol. 1970 Nov;105(5):1253-63
- J Biol Chem. 1971 Aug 10;246(15):4703-12
- J Immunol. 1973 Jan;110(1):262-8
- Immunochemistry. 1973 Jun;10(6):365-71
- N Engl J Med. 1974 May 30;290(22):1216-20
- J Biol Chem. 1975 Mar 10;250(5):1926-32
- J Immunol. 1975 Jan;114(1 Pt 1):99-101
- Immunology. 1975 Jan;28(1):187-97
- Lancet. 1975 May 17;1(7916):1099-104
- Acta Pathol Microbiol Scand B. 1977 Feb;85B(1):103-7
- Immunochemistry. 1977 Mar;14(3):161-4
- Infect Immun. 1977 Apr;16(1):75-80
- J Clin Pathol. 1977 Sep;30(9):834-7
- J Clin Microbiol. 1978 May;7(5):410-4
- J Exp Med. 1979 Mar 1;149(3):669-85
- J Clin Microbiol. 1980 Oct;12(4):498-501
Citations
This article has been cited 9 times.- Vionnet J, Peterson DC, Loy JD, Wynn E, Battistel MD, Hille M, Clawson ML, Vann W. Identification of Polysialic Acid and Chondroitin-like Polysaccharides of Moraxella bovis Strains Associated with Infectious Bovine Keratoconjunctivitis. ACS Infect Dis 2026 Jan 9;12(1):152-161.
- Girgis MM, Christodoulides M. Vertebrate and Invertebrate Animal and New In Vitro Models for Studying Neisseria Biology. Pathogens 2023 May 30;12(6).
- Robbins JB, Schneerson R, Xie G, Hanson LÅ, Miller MA. Capsular polysaccharide vaccine for Group B Neisseria meningitidis, Escherichia coli K1, and Pasteurella haemolytica A2. Proc Natl Acad Sci U S A 2011 Nov 1;108(44):17871-5.
- Goller CC, Seed PC. High-throughput identification of chemical inhibitors of E. coli Group 2 capsule biogenesis as anti-virulence agents. PLoS One 2010 Jul 19;5(7):e11642.
- Erwin AL, Gotschlich EC. Oxidation of D-lactate and L-lactate by Neisseria meningitidis: purification and cloning of meningococcal D-lactate dehydrogenase. J Bacteriol 1993 Oct;175(20):6382-91.
- Diaz Romero J, Outschoorn IM. Current status of meningococcal group B vaccine candidates: capsular or noncapsular?. Clin Microbiol Rev 1994 Oct;7(4):559-75.
- Kabat EA, Nickerson KG, Liao J, Grossbard L, Osserman EF, Glickman E, Chess L, Robbins JB, Schneerson R, Yang YH. A human monoclonal macroglobulin with specificity for alpha(2----8)-linked poly-N-acetyl neuraminic acid, the capsular polysaccharide of group B meningococci and Escherichia coli K1, which crossreacts with polynucleotides and with denatured DNA. J Exp Med 1986 Aug 1;164(2):642-54.
- Devi SJ, Robbins JB, Schneerson R. Antibodies to poly[(2----8)-alpha-N-acetylneuraminic acid] and poly[(2----9)-alpha-N-acetylneuraminic acid] are elicited by immunization of mice with Escherichia coli K92 conjugates: potential vaccines for groups B and C meningococci and E. coli K1. Proc Natl Acad Sci U S A 1991 Aug 15;88(16):7175-9.
- Devi SJ, Schneerson R, Egan W, Vann WF, Robbins JB, Shiloach J. Identity between polysaccharide antigens of Moraxella nonliquefaciens, group B Neisseria meningitidis, and Escherichia coli K1 (non-O acetylated). Infect Immun 1991 Feb;59(2):732-6.
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