Identification of lipoprotein homologues of pneumococcal PsaA in the equine pathogens Streptococcus equi and Streptococcus zooepidemicus.
Abstract: Streptococcus equi and Streptococcus zooepidemicus are major etiological agents of upper and lower airway disease in horses. Despite the considerable animal suffering and economic burden associated with these diseases, the factors that contribute to the virulence of these equine pathogens have not been extensively investigated. Here we demonstrate the presence of a homologue of the Streptococcus pneumoniae PsaA protein in both of these equine pathogens. Inhibition of signal peptide processing by the antibiotic globomycin confirmed the lipoprotein nature of the mature proteins, and surface exposure was confirmed by their release from intact cells by mild trypsinolysis.
Publication Date: 2000-09-19 PubMed ID: 10992520PubMed Central: PMC101572DOI: 10.1128/IAI.68.10.6048-6051.2000Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research looked at two major causes of airway disease in horses, Streptococcus equi and Streptococcus zooepidemicus, finding a protein common to both that is similar to one found in Streptococcus pneumoniae in humans. Their research determined this protein has a lipoprotein nature, and it was found on the surface of the cells, suggesting a role in virulence.
Understanding the Context
- Streptococcus equi and Streptococcus zooepidemicus are bacterial pathogens that primarily affect horses, causing severe upper and lower respiratory tract infections. These infections can lead to significant disease and death in horses, causing both animal welfare issues and economic loss.
- To date, the factors contributing to these bacteria’s virulence (the degree to which they cause disease) have not been extensively studied.
Findings of the Study
- The researchers found a homologue of the Streptococcus pneumoniae PsaA protein in both Streptococcus equi and Streptococcus zooepidemicus. A homologue is a gene or protein that shares a common evolutionary origin. In this case, the PsaA protein of Streptococcus pneumoniae (a human pathogen) was found to have a comparable protein in both equine pathogens.
- The presence of this protein in all three types of Streptococcus suggests a common functionality or role in the pathogenic process.
Characterization of the Identified Protein
- The researchers used globomycin, an antibiotic, to inhibit signal peptide processing, a crucial step in the maturation of proteins. This confirmed that the mature form of the identified proteins in Streptococcus equi and Streptococcus zooepidemicus is lipoprotein in nature.
- Lipoproteins are proteins covalently bound to lipids and are commonly found in bacterial cell membranes. They play critical roles in various cellular functions, including acting as enzymes, receptors, and transporters.
- The surface exposure of these proteins was confirmed by their release from intact cells through mild trypsinolysis, an enzyme-based digestion process. This suggests that the proteins are on the cells’ outer surface, likely making them accessible for interactions with the host during infections, and potentially playing a role in the virulence of these pathogens.
Cite This Article
APA
Harrington DJ, Greated JS, Chanter N, Sutcliffe IC.
(2000).
Identification of lipoprotein homologues of pneumococcal PsaA in the equine pathogens Streptococcus equi and Streptococcus zooepidemicus.
Infect Immun, 68(10), 6048-6051.
https://doi.org/10.1128/IAI.68.10.6048-6051.2000 Publication
Researcher Affiliations
- School of Sciences, University of Sunderland, Sunderland, United Kingdom. dean.harrington@sunderland.ac.uk
MeSH Terms
- Adhesins, Bacterial
- Animals
- Bacterial Proteins / chemistry
- Bacterial Proteins / genetics
- Bacterial Proteins / metabolism
- Carrier Proteins / chemistry
- Carrier Proteins / genetics
- Carrier Proteins / metabolism
- Horse Diseases / microbiology
- Horses
- Lipoproteins / chemistry
- Lipoproteins / genetics
- Lipoproteins / metabolism
- Membrane Transport Proteins
- Molecular Sequence Data
- Sequence Homology, Amino Acid
- Streptococcal Infections / microbiology
- Streptococcal Infections / veterinary
- Streptococcus equi / genetics
- Streptococcus equi / pathogenicity
- Virulence
Grant Funding
- Wellcome Trust
References
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Citations
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