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Home / Equine Research Bank / J Virol / Identification of the activation domain of equine infectious...
Journal of virology1993; 67(12); 7317-7323; doi: 10.1128/JVI.67.12.7317-7323.1993

Identification of the activation domain of equine infectious anemia virus rev.

Abstract: Several members of the lentivirus family of complex retroviruses have been shown to encode proteins that are functionally equivalent to the Rev posttranscriptional regulatory protein of human immunodeficiency virus type 1 (HIV-1). Furthermore, the domain organization of HIV-1 Rev, featuring a highly basic N-terminal RNA binding domain and a leucin-rich C-terminal effector domain, has also been shown to be highly conserved among Rev proteins derived from not only the primate but also the ovine and caprine lentiviruses. Although it has therefore appeared highly probable that the lentivirus equine infectious anemia virus (EIAV) also encodes a Rev, the predicted amino acid sequence of this putative EIAV regulatory protein does not display any evident homology to the basic and leucine-rich motifs characteristic of other known Rev proteins. By fusion of different segments of the proposed EIAV Rev protein to the well-defined RNA binding domain of either HIV-1 or visna virus Rev, we have identified a segment of this EIAV protein that can efficiently substitute in cis for the otherwise essential activation motif. Interestingly, the minimal EIAV Rev activation motif identified in this study comprises approximately 18 amino acids located toward the protein N terminus that lack any evident similarity to the leucine-rich activation domains found in these other lentivirus Rev proteins. It therefore appears that the Rev protein of EIAV, while analogous in function to Rev proteins defined in lentiviruses of primate, ovine, and caprine origin, is nevertheless distinguished by an entirely novel domain organization.
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Publication Date: 1993-12-01 PubMed ID: 8230455PubMed Central: PMC238195DOI: 10.1128/JVI.67.12.7317-7323.1993Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the activation domain of a regulatory protein, Rev, in the lentivirus equine infectious anemia virus (EIAV). This protein is found to have a unique structure that differs from other Rev proteins in the lentivirus family.

Introduction of the Research

  • The article studies the Rev posttranscriptional regulatory protein found in the lentivirus family of complex retroviruses. This protein has been identified in other members of the lentivirus family, including the human immunodeficiency virus type 1 (HIV-1).
  • Although the domain structure of Rev, characterized by an N-terminal RNA binding domain, and a leucin-rich C-terminal effector domain, is conserved across primate, ovine, and caprine lentiviruses, it is suggested that there’s a difference in the structure of Rev in EIAV.

Study of EIAV’s Rev Protein

  • The researchers examined EIAV’s Rev protein as the predicted amino acid sequence of this protein doesn’t display evident homology to the leucine-rich and basic motifs that are characteristic of other known Rev proteins.
  • To understand the structure of EIAV’s Rev, researchers fused different segments of this protein to the well-defined RNA binding domain of either HIV-1 or visna virus Rev.
  • Through this, they identified a segment of the EIAV protein that can efficiently function as the activation motif, a key component of the Rev protein.

Findings About EIAV’s Rev

  • The researchers found that the minimal activation motif of EIAV’s Rev contains about 18 amino acids that are located toward the protein’s N terminus.
  • Interestingly, this motif doesn’t display evident similarity to the leucine-rich activation domains typically found in other lentivirus Rev proteins.
  • This suggests that, while the function of EIAV’s Rev mirrors that of Rev proteins from lentiviruses of primate, ovine, and caprine origin, its domain organization is entirely novel.

Cite This Article

APA
Fridell RA, Partin KM, Carpenter S, Cullen BR. (1993). Identification of the activation domain of equine infectious anemia virus rev. J Virol, 67(12), 7317-7323. https://doi.org/10.1128/JVI.67.12.7317-7323.1993

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 67
Issue: 12
Pages: 7317-7323

Researcher Affiliations

Fridell, R A
  • Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
Partin, K M
    Carpenter, S
      Cullen, B R

        MeSH Terms

        • Amino Acid Sequence
        • Animals
        • Cell Line
        • Cloning, Molecular
        • Gene Expression Regulation, Viral
        • Genes, rev
        • HIV-1 / genetics
        • Infectious Anemia Virus, Equine / genetics
        • Molecular Sequence Data
        • Open Reading Frames / genetics
        • Recombinant Fusion Proteins
        • Regulatory Sequences, Nucleic Acid
        • Visna-maedi virus / genetics

        Grant Funding

        • AI-28233 / NIAID NIH HHS
        • AI-30025 / NIAID NIH HHS

        References

        This article includes 40 references
        1. Rushlow K, Olsen K, Stiegler G, Payne SL, Montelaro RC, Issel CJ. Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus.. Virology 1986 Dec;155(2):309-21.
          pubmed: 2431539doi: 10.1016/0042-6822(86)90195-9google scholar: lookup
        2. Feinberg MB, Jarrett RF, Aldovini A, Gallo RC, Wong-Staal F. HTLV-III expression and production involve complex regulation at the levels of splicing and translation of viral RNA.. Cell 1986 Sep 12;46(6):807-17.
          pubmed: 3638988doi: 10.1016/0092-8674(86)90062-0google scholar: lookup
        3. Cullen BR. Use of eukaryotic expression technology in the functional analysis of cloned genes.. Methods Enzymol 1987;152:684-704.
          pubmed: 3657593doi: 10.1016/0076-6879(87)52074-2google scholar: lookup
        4. Davis JL, Clements JE. Characterization of a cDNA clone encoding the visna virus transactivating protein.. Proc Natl Acad Sci U S A 1989 Jan;86(2):414-8.
          pubmed: 2536163doi: 10.1073/pnas.86.2.414google scholar: lookup
        5. Malim MH, Hauber J, Le SY, Maizel JV, Cullen BR. The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA.. Nature 1989 Mar 16;338(6212):254-7.
          pubmed: 2784194doi: 10.1038/338254a0google scholar: lookup
        6. Felber BK, Hadzopoulou-Cladaras M, Cladaras C, Copeland T, Pavlakis GN. rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA.. Proc Natl Acad Sci U S A 1989 Mar;86(5):1495-9.
          pubmed: 2784208doi: 10.1073/pnas.86.5.1495google scholar: lookup
        7. Malim MH, Böhnlein S, Hauber J, Cullen BR. Functional dissection of the HIV-1 Rev trans-activator--derivation of a trans-dominant repressor of Rev function.. Cell 1989 Jul 14;58(1):205-14.
          pubmed: 2752419doi: 10.1016/0092-8674(89)90416-9google scholar: lookup
        8. Kubota S, Siomi H, Satoh T, Endo S, Maki M, Hatanaka M. Functional similarity of HIV-I rev and HTLV-I rex proteins: identification of a new nucleolar-targeting signal in rev protein.. Biochem Biophys Res Commun 1989 Aug 15;162(3):963-70.
          pubmed: 2788417doi: 10.1016/0006-291x(89)90767-5google scholar: lookup
        9. Malim MH, Böhnlein S, Fenrick R, Le SY, Maizel JV, Cullen BR. Functional comparison of the Rev trans-activators encoded by different primate immunodeficiency virus species.. Proc Natl Acad Sci U S A 1989 Nov;86(21):8222-6.
          pubmed: 2682638doi: 10.1073/pnas.86.21.8222google scholar: lookup
        10. Chang DD, Sharp PA. Regulation by HIV Rev depends upon recognition of splice sites.. Cell 1989 Dec 1;59(5):789-95.
          pubmed: 2686839doi: 10.1016/0092-8674(89)90602-8google scholar: lookup
        11. Rasty S, Dhruva BR, Schiltz RL, Shih DS, Issel CJ, Montelaro RC. Proviral DNA integration and transcriptional patterns of equine infectious anemia virus during persistent and cytopathic infections.. J Virol 1990 Jan;64(1):86-95.
          pubmed: 2152836doi: 10.1128/JVI.64.1.86-95.1990google scholar: lookup
        12. Malim MH, Tiley LS, McCarn DF, Rusche JR, Hauber J, Cullen BR. HIV-1 structural gene expression requires binding of the Rev trans-activator to its RNA target sequence.. Cell 1990 Feb 23;60(4):675-83.
          pubmed: 2406030doi: 10.1016/0092-8674(90)90670-agoogle scholar: lookup
        13. Dorn P, DaSilva L, Martarano L, Derse D. Equine infectious anemia virus tat: insights into the structure, function, and evolution of lentivirus trans-activator proteins.. J Virol 1990 Apr;64(4):1616-24.
          pubmed: 2157047doi: 10.1128/JVI.64.4.1616-1624.1990google scholar: lookup
        14. Stephens RM, Derse D, Rice NR. Cloning and characterization of cDNAs encoding equine infectious anemia virus tat and putative Rev proteins.. J Virol 1990 Aug;64(8):3716-25.
          pubmed: 2164593doi: 10.1128/JVI.64.8.3716-3725.1990google scholar: lookup
        15. Dillon PJ, Nelbock P, Perkins A, Rosen CA. Function of the human immunodeficiency virus types 1 and 2 Rev proteins is dependent on their ability to interact with a structured region present in env gene mRNA.. J Virol 1990 Sep;64(9):4428-37.
          pubmed: 2200888doi: 10.1128/JVI.64.9.4428-4437.1990google scholar: lookup
        16. Venkatesh LK, Chinnadurai G. Mutants in a conserved region near the carboxy-terminus of HIV-1 Rev identify functionally important residues and exhibit a dominant negative phenotype.. Virology 1990 Sep;178(1):327-30.
          pubmed: 2202148doi: 10.1016/0042-6822(90)90414-mgoogle scholar: lookup
        17. Tiley LS, Brown PH, Le SY, Maizel JV, Clements JE, Cullen BR. Visna virus encodes a post-transcriptional regulator of viral structural gene expression.. Proc Natl Acad Sci U S A 1990 Oct;87(19):7497-501.
          pubmed: 2170981doi: 10.1073/pnas.87.19.7497google scholar: lookup
        18. Lu XB, Heimer J, Rekosh D, Hammarskjöld ML. U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced.. Proc Natl Acad Sci U S A 1990 Oct;87(19):7598-602.
          pubmed: 2217190doi: 10.1073/pnas.87.19.7598google scholar: lookup
        19. Hope TJ, Huang XJ, McDonald D, Parslow TG. Steroid-receptor fusion of the human immunodeficiency virus type 1 Rev transactivator: mapping cryptic functions of the arginine-rich motif.. Proc Natl Acad Sci U S A 1990 Oct;87(19):7787-91.
          pubmed: 2217212doi: 10.1073/pnas.87.19.7787google scholar: lookup
        20. Olsen HS, Cochrane AW, Dillon PJ, Nalin CM, Rosen CA. Interaction of the human immunodeficiency virus type 1 Rev protein with a structured region in env mRNA is dependent on multimer formation mediated through a basic stretch of amino acids.. Genes Dev 1990 Aug;4(8):1357-64.
          pubmed: 2227413doi: 10.1101/gad.4.8.1357google scholar: lookup
        21. Dillon PJ, Nelbock P, Perkins A, Rosen CA. Structural and functional analysis of the human immunodeficiency virus type 2 Rev protein.. J Virol 1991 Jan;65(1):445-9.
          pubmed: 1985207doi: 10.1128/JVI.65.1.445-449.1991google scholar: lookup
        22. Carpenter S, Alexandersen S, Long MJ, Perryman S, Chesebro B. Identification of a hypervariable region in the long terminal repeat of equine infectious anemia virus.. J Virol 1991 Mar;65(3):1605-10.
          pubmed: 1847479doi: 10.1128/JVI.65.3.1605-1610.1991google scholar: lookup
        23. Malim MH, Cullen BR. HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: implications for HIV-1 latency.. Cell 1991 Apr 19;65(2):241-8.
          pubmed: 2015625doi: 10.1016/0092-8674(91)90158-ugoogle scholar: lookup
        24. Carroll R, Martarano L, Derse D. Identification of lentivirus tat functional domains through generation of equine infectious anemia virus/human immunodeficiency virus type 1 tat gene chimeras.. J Virol 1991 Jul;65(7):3460-7.
          pubmed: 1645777doi: 10.1128/JVI.65.7.3460-3467.1991google scholar: lookup
        25. Tiley LS, Malim MH, Cullen BR. Conserved functional organization of the human immunodeficiency virus type 1 and visna virus Rev proteins.. J Virol 1991 Jul;65(7):3877-81.
          pubmed: 1645796doi: 10.1128/JVI.65.7.3877-3881.1991google scholar: lookup
        26. Malim MH, McCarn DF, Tiley LS, Cullen BR. Mutational definition of the human immunodeficiency virus type 1 Rev activation domain.. J Virol 1991 Aug;65(8):4248-54.
          pubmed: 2072452doi: 10.1128/JVI.65.8.4248-4254.1991google scholar: lookup
        27. Kiyomasu T, Miyazawa T, Furuya T, Shibata R, Sakai H, Sakuragi J, Fukasawa M, Maki N, Hasegawa A, Mikami T. Identification of feline immunodeficiency virus rev gene activity.. J Virol 1991 Aug;65(8):4539-42.
          pubmed: 1649349doi: 10.1128/JVI.65.8.4539-4542.1991google scholar: lookup
        28. Kalinski H, Yaniv A, Mashiah P, Miki T, Tronick SR, Gazit A. rev-like transcripts of caprine arthritis encephalitis virus.. Virology 1991 Aug;183(2):786-92.
          pubmed: 1649509doi: 10.1016/0042-6822(91)91012-6google scholar: lookup
        29. Zapp ML, Hope TJ, Parslow TG, Green MR. Oligomerization and RNA binding domains of the type 1 human immunodeficiency virus Rev protein: a dual function for an arginine-rich binding motif.. Proc Natl Acad Sci U S A 1991 Sep 1;88(17):7734-8.
          pubmed: 1715576doi: 10.1073/pnas.88.17.7734google scholar: lookup
        30. Kjems J, Frankel AD, Sharp PA. Specific regulation of mRNA splicing in vitro by a peptide from HIV-1 Rev.. Cell 1991 Oct 4;67(1):169-78.
          pubmed: 1913815doi: 10.1016/0092-8674(91)90580-rgoogle scholar: lookup
        31. Hope TJ, Bond BL, McDonald D, Klein NP, Parslow TG. Effector domains of human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex are functionally interchangeable and share an essential peptide motif.. J Virol 1991 Nov;65(11):6001-7.
          pubmed: 1920623doi: 10.1128/JVI.65.11.6001-6007.1991google scholar: lookup
        32. Böhnlein E, Berger J, Hauber J. Functional mapping of the human immunodeficiency virus type 1 Rev RNA binding domain: new insights into the domain structure of Rev and Rex.. J Virol 1991 Dec;65(12):7051-5.
          pubmed: 1942257doi: 10.1128/JVI.65.12.7051-7055.1991google scholar: lookup
        33. Weichselbraun I, Farrington GK, Rusche JR, Böhnlein E, Hauber J. Definition of the human immunodeficiency virus type 1 Rev and human T-cell leukemia virus type I Rex protein activation domain by functional exchange.. J Virol 1992 Apr;66(4):2583-7.
          pubmed: 1548784doi: 10.1128/JVI.66.4.2583-2587.1992google scholar: lookup
        34. Eustice DC, Feldman PA, Colberg-Poley AM, Buckery RM, Neubauer RH. A sensitive method for the detection of beta-galactosidase in transfected mammalian cells.. Biotechniques 1991 Dec;11(6):739-40, 742-3.
          pubmed: 1809326
        35. Tiley LS, Cullen BR. Structural and functional analysis of the visna virus Rev-response element.. J Virol 1992 Jun;66(6):3609-15.
          pubmed: 1316470doi: 10.1128/JVI.66.6.3609-3615.1992google scholar: lookup
        36. Garrett ED, Cullen BR. Comparative analysis of Rev function in human immunodeficiency virus types 1 and 2.. J Virol 1992 Jul;66(7):4288-94.
          pubmed: 1602545doi: 10.1128/JVI.66.7.4288-4294.1992google scholar: lookup
        37. Phillips TR, Lamont C, Konings DA, Shacklett BL, Hamson CA, Luciw PA, Elder JH. Identification of the Rev transactivation and Rev-responsive elements of feline immunodeficiency virus.. J Virol 1992 Sep;66(9):5464-71.
          pubmed: 1323707doi: 10.1128/JVI.66.9.5464-5471.1992google scholar: lookup
        38. Carroll R, Derse D. Translation of equine infectious anemia virus bicistronic tat-rev mRNA requires leaky ribosome scanning of the tat CTG initiation codon.. J Virol 1993 Mar;67(3):1433-40.
          pubmed: 8382305doi: 10.1128/JVI.67.3.1433-1440.1993google scholar: lookup
        39. Sodroski J, Goh WC, Rosen C, Dayton A, Terwilliger E, Haseltine W. A second post-transcriptional trans-activator gene required for HTLV-III replication.. Nature 1986 May 22-28;321(6068):412-7.
          pubmed: 3012355doi: 10.1038/321412a0google scholar: lookup
        40. Kawakami T, Sherman L, Dahlberg J, Gazit A, Yaniv A, Tronick SR, Aaronson SA. Nucleotide sequence analysis of equine infectious anemia virus proviral DNA.. Virology 1987 Jun;158(2):300-12.
          pubmed: 3035786doi: 10.1016/0042-6822(87)90202-9google scholar: lookup

        Citations

        This article has been cited 35 times.
        1. Li J, Zhang X, Bai B, Zhang M, Ma W, Lin Y, Wang X, Wang XF. Identification of a Novel Post-transcriptional Transactivator from the Equine Infectious Anemia Virus.. J Virol 2022 Dec 21;96(24):e0121022.
          doi: 10.1128/jvi.01210-22pubmed: 36448796google scholar: lookup
        2. Ganser LR, Chu CC, Bogerd HP, Kelly ML, Cullen BR, Al-Hashimi HM. Probing RNA Conformational Equilibria within the Functional Cellular Context.. Cell Rep 2020 Feb 25;30(8):2472-2480.e4.
          doi: 10.1016/j.celrep.2020.02.004pubmed: 32101729google scholar: lookup
        3. Sherpa C, Rausch JW, Le Grice SF, Hammarskjold ML, Rekosh D. The HIV-1 Rev response element (RRE) adopts alternative conformations that promote different rates of virus replication.. Nucleic Acids Res 2015 May 19;43(9):4676-86.
          doi: 10.1093/nar/gkv313pubmed: 25855816google scholar: lookup
        4. Stake MS, Bann DV, Kaddis RJ, Parent LJ. Nuclear trafficking of retroviral RNAs and Gag proteins during late steps of replication.. Viruses 2013 Nov 18;5(11):2767-95.
          doi: 10.3390/v5112767pubmed: 24253283google scholar: lookup
        5. Farley DC, Bannister R, Leroux-Carlucci MA, Evans NE, Miskin JE, Mitrophanous KA. Development of an equine-tropic replication-competent lentivirus assay for equine infectious anemia virus-based lentiviral vectors.. Hum Gene Ther Methods 2012 Oct;23(5):309-23.
          doi: 10.1089/hgtb.2012.102pubmed: 23121195google scholar: lookup
        6. Carpenter S, Chen WC, Dorman KS. Rev variation during persistent lentivirus infection.. Viruses 2011 Jan;3(1):1-11.
          doi: 10.3390/v3010001pubmed: 21994723google scholar: lookup
        7. Hammarskjold MH, Rekosh D. A long-awaited structure is rev-ealed.. Viruses 2011 May;3(5):484-92.
          doi: 10.3390/v3050484pubmed: 21941623google scholar: lookup
        8. Ihm Y, Sparks WO, Lee JH, Cao H, Carpenter S, Wang CZ, Ho KM, Dobbs D. Structural model of the Rev regulatory protein from equine infectious anemia virus.. PLoS One 2009;4(1):e4178.
          doi: 10.1371/journal.pone.0004178pubmed: 19137065google scholar: lookup
        9. Bogerd HP, Tallmadge RL, Oaks JL, Carpenter S, Cullen BR. Equine infectious anemia virus resists the antiretroviral activity of equine APOBEC3 proteins through a packaging-independent mechanism.. J Virol 2008 Dec;82(23):11889-901.
          doi: 10.1128/JVI.01537-08pubmed: 18818324google scholar: lookup
        10. Lee JH, Culver G, Carpenter S, Dobbs D. Analysis of the EIAV Rev-responsive element (RRE) reveals a conserved RNA motif required for high affinity Rev binding in both HIV-1 and EIAV.. PLoS One 2008 Jun 4;3(6):e2272.
          doi: 10.1371/journal.pone.0002272pubmed: 18523581google scholar: lookup
        11. Sparks WO, Dorman KS, Liu S, Carpenter S. Naturally arising point mutations in non-essential domains of equine infectious anemia virus Rev alter Rev-dependent nuclear-export activity.. J Gen Virol 2008 Apr;89(Pt 4):1043-1048.
          doi: 10.1099/vir.0.83195-0pubmed: 18343848google scholar: lookup
        12. Ribet D, Harper F, Dupressoir A, Dewannieux M, Pierron G, Heidmann T. An infectious progenitor for the murine IAP retrotransposon: emergence of an intracellular genetic parasite from an ancient retrovirus.. Genome Res 2008 Apr;18(4):597-609.
          doi: 10.1101/gr.073486.107pubmed: 18256233google scholar: lookup
        13. Ribet D, Harper F, Dewannieux M, Pierron G, Heidmann T. Murine MusD retrotransposon: structure and molecular evolution of an "intracellularized" retrovirus.. J Virol 2007 Feb;81(4):1888-98.
          doi: 10.1128/JVI.02051-06pubmed: 17151128google scholar: lookup
        14. Terribilini M, Lee JH, Yan C, Jernigan RL, Carpenter S, Honavar V, Dobbs D. Identifying interaction sites in "recalcitrant" proteins: predicted protein and RNA binding sites in rev proteins of HIV-1 and EIAV agree with experimental data.. Pac Symp Biocomput 2006;:415-26.
          pubmed: 17094257
        15. Lee JH, Murphy SC, Belshan M, Sparks WO, Wannemuehler Y, Liu S, Hope TJ, Dobbs D, Carpenter S. Characterization of functional domains of equine infectious anemia virus Rev suggests a bipartite RNA-binding domain.. J Virol 2006 Apr;80(8):3844-52.
          doi: 10.1128/JVI.80.8.3844-3852.2006pubmed: 16571801google scholar: lookup
        16. Mealey RH, Zhang B, Leib SR, Littke MH, McGuire TC. Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus.. Virology 2003 Sep 1;313(2):537-52.
          doi: 10.1016/s0042-6822(03)00344-1pubmed: 12954220google scholar: lookup
        17. Rowland RR, Yoo D. Nucleolar-cytoplasmic shuttling of PRRSV nucleocapsid protein: a simple case of molecular mimicry or the complex regulation by nuclear import, nucleolar localization and nuclear export signal sequences.. Virus Res 2003 Sep;95(1-2):23-33.
          doi: 10.1016/s0168-1702(03)00161-8pubmed: 12921993google scholar: lookup
        18. Yang J, Bogerd H, Le SY, Cullen BR. The human endogenous retrovirus K Rev response element coincides with a predicted RNA folding region.. RNA 2000 Nov;6(11):1551-64.
          doi: 10.1017/s135583820000100xpubmed: 11105755google scholar: lookup
        19. Jensen TH, Neville M, Rain JC, McCarthy T, Legrain P, Rosbash M. Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling.. Mol Cell Biol 2000 Nov;20(21):8047-58.
          doi: 10.1128/MCB.20.21.8047-8058.2000pubmed: 11027275google scholar: lookup
        20. Belshan M, Park GS, Bilodeau P, Stoltzfus CM, Carpenter S. Binding of equine infectious anemia virus rev to an exon splicing enhancer mediates alternative splicing and nuclear export of viral mRNAs.. Mol Cell Biol 2000 May;20(10):3550-7.
          doi: 10.1128/MCB.20.10.3550-3557.2000pubmed: 10779344google scholar: lookup
        21. Yang J, Cullen BR. Structural and functional analysis of the avian leukemia virus constitutive transport element.. RNA 1999 Dec;5(12):1645-55.
          doi: 10.1017/s1355838299991616pubmed: 10606274google scholar: lookup
        22. Yang J, Bogerd HP, Peng S, Wiegand H, Truant R, Cullen BR. An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev protein.. Proc Natl Acad Sci U S A 1999 Nov 9;96(23):13404-8.
          doi: 10.1073/pnas.96.23.13404pubmed: 10557333google scholar: lookup
        23. Bear J, Tan W, Zolotukhin AS, Tabernero C, Hudson EA, Felber BK. Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.. Mol Cell Biol 1999 Sep;19(9):6306-17.
          doi: 10.1128/MCB.19.9.6306pubmed: 10454577google scholar: lookup
        24. Bogerd HP, Echarri A, Ross TM, Cullen BR. Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1.. J Virol 1998 Nov;72(11):8627-35.
          doi: 10.1128/JVI.72.11.8627-8635.1998pubmed: 9765402google scholar: lookup
        25. Otero GC, Harris ME, Donello JE, Hope TJ. Leptomycin B inhibits equine infectious anemia virus Rev and feline immunodeficiency virus rev function but not the function of the hepatitis B virus posttranscriptional regulatory element.. J Virol 1998 Sep;72(9):7593-7.
          doi: 10.1128/JVI.72.9.7593-7597.1998pubmed: 9696859google scholar: lookup
        26. Harris ME, Gontarek RR, Derse D, Hope TJ. Differential requirements for alternative splicing and nuclear export functions of equine infectious anemia virus Rev protein.. Mol Cell Biol 1998 Jul;18(7):3889-99.
          doi: 10.1128/MCB.18.7.3889pubmed: 9632773google scholar: lookup
        27. Engel K, Kotlyarov A, Gaestel M. Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation.. EMBO J 1998 Jun 15;17(12):3363-71.
          doi: 10.1093/emboj/17.12.3363pubmed: 9628873google scholar: lookup
        28. Belshan M, Harris ME, Shoemaker AE, Hope TJ, Carpenter S. Biological characterization of Rev variation in equine infectious anemia virus.. J Virol 1998 May;72(5):4421-6.
          doi: 10.1128/JVI.72.5.4421-4426.1998pubmed: 9557734google scholar: lookup
        29. Cook RF, Leroux C, Cook SJ, Berger SL, Lichtenstein DL, Ghabrial NN, Montelaro RC, Issel CJ. Development and characterization of an in vivo pathogenic molecular clone of equine infectious anemia virus.. J Virol 1998 Feb;72(2):1383-93.
          doi: 10.1128/JVI.72.2.1383-1393.1998pubmed: 9445039google scholar: lookup
        30. Leroux C, Issel CJ, Montelaro RC. Novel and dynamic evolution of equine infectious anemia virus genomic quasispecies associated with sequential disease cycles in an experimentally infected pony.. J Virol 1997 Dec;71(12):9627-39.
          doi: 10.1128/JVI.71.12.9627-9639.1997pubmed: 9371627google scholar: lookup
        31. Kim FJ, Beeche AA, Hunter JJ, Chin DJ, Hope TJ. Characterization of the nuclear export signal of human T-cell lymphotropic virus type 1 Rex reveals that nuclear export is mediated by position-variable hydrophobic interactions.. Mol Cell Biol 1996 Sep;16(9):5147-55.
          doi: 10.1128/MCB.16.9.5147pubmed: 8756672google scholar: lookup
        32. Bogerd HP, Fridell RA, Benson RE, Hua J, Cullen BR. Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay.. Mol Cell Biol 1996 Aug;16(8):4207-14.
          doi: 10.1128/MCB.16.8.4207pubmed: 8754820google scholar: lookup
        33. Meyer BE, Meinkoth JL, Malim MH. Nuclear transport of human immunodeficiency virus type 1, visna virus, and equine infectious anemia virus Rev proteins: identification of a family of transferable nuclear export signals.. J Virol 1996 Apr;70(4):2350-9.
          doi: 10.1128/JVI.70.4.2350-2359.1996pubmed: 8642662google scholar: lookup
        34. Luo Y, Yu H, Peterlin BM. Cellular protein modulates effects of human immunodeficiency virus type 1 Rev.. J Virol 1994 Jun;68(6):3850-6.
          doi: 10.1128/JVI.68.6.3850-3856.1994pubmed: 8189522google scholar: lookup
        35. Simon JH, Southerling TE, Peterson JC, Meyer BE, Malim MH. Complementation of vif-defective human immunodeficiency virus type 1 by primate, but not nonprimate, lentivirus vif genes.. J Virol 1995 Jul;69(7):4166-72.
          doi: 10.1128/JVI.69.7.4166-4172.1995pubmed: 7769676google scholar: lookup
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