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Home / Equine Research Bank / Viruses / Identification of the Promoter Antisense Transcript Enhancin...
Viruses2024; 16(8); doi: 10.3390/v16081195

Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus-1 Immediate-Early Gene.

Abstract: Equine herpesvirus-1 (EHV-1) causes respiratory diseases, abortion, and encephalomyelitis in horses. The EHV-1 immediate-early (IE) protein, essential for viral replication, is transactivated by the binding of a multiprotein complex including the open reading frame 12 (ORF12) and some host factors to the IE promoter region. Promoter-associated non-coding RNAs (pancRNAs), which are transcribed from bidirectional promoters, regulate the transcription of neighboring genes in mammals and pathogens. In this study, we identified a novel pancRNA transcribed from across the areas of the 5'-untranslated region and a promoter of EHV-1 IE and named it IE pancRNA. IE pancRNA and mRNA were simultaneously expressed in EHV-1-infected RN33B-A68B2M cells. This pancRNA was also transcribed in RK13 and E. Derm cells, which are highly susceptible to EHV-1 infection. Furthermore, IE pancRNA upregulated IE gene expression in the presence of ORF12, and stable expression of IE pancRNA increased the number of EHV-1-infected RN33B-A68B2M cells. These results suggest that IE pancRNAs facilitate EHV-1 proliferation by promoting IE gene expression.
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Publication Date: 2024-07-25 PubMed ID: 39205169PubMed Central: PMC11360796DOI: 10.3390/v16081195Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study focuses on the discovery of a promoter antisense transcript, namely IE pancRNA, which enhances the transcription of the immediate-early (IE) gene of Equine herpesvirus-1 (EHV-1), thus influencing the proliferation of the virus in horses resulting in several diseases.

Research Background

  • The Equine herpesvirus-1 (EHV-1) is known for causing respiratory diseases, abortion, and encephalomyelitis in horses. The replication of this virus depends on the immediate-early (IE) protein, which is invoked via the binding of a multiprotein complex to the IE promoter region. This complex includes the open reading frame 12 (ORF12) and some host factors.
  • Promoter-associated non-coding RNAs (pancRNAs) are an interesting group of RNA which do not get translated into proteins but help regulate the transcription of nearby genes. Discerning the role of pancRNAs is important for understanding the gene regulation of mammals as well as pathogens.

Discovery and Role of IE pancRNA

  • In this study, researchers identified a novel pancRNA related to the EHV-1 virus and named it IE pancRNA. This was found to be transcribed from across the areas of the 5′ (five-prime) untranslated region and a promoter of EHV-1 IE.
  • The IE pancRNA and mRNA expressions were found to occur simultaneously in the EHV-1 infected cells of RN33B-A68B2M. This pancRNA was also found to be transcribed in RK13 and E. Derm cells that are highly susceptible to EHV-1 infection. The presence of IE pancRNA led to an upsurge in gene expression when ORF12 was present.

Implications of the IE pancRNA

  • Further, it was observed that the stable expression of IE pancRNA led to an increase in the count of EHV-1 infected RN33B-A68B2M cells. This suggested that the IE pancRNA plays a pivotal role in facilitating the proliferation of EHV-1 by boosting gene expression.
  • Through this research, it becomes clear that understanding these promoter antisense transcripts could be crucial in combating diseases caused by EHV-1 in horses.

Cite This Article

APA
Maeda M, Abe M, Aoshima K, Kobayashi A, Fukushi H, Kimura T. (2024). Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus-1 Immediate-Early Gene. Viruses, 16(8). https://doi.org/10.3390/v16081195

Publication

Viruses
ISSN: 1999-4915
NlmUniqueID: 101509722
Country: Switzerland
Language: English
Volume: 16
Issue: 8

Researcher Affiliations

Maeda, Mayuko
  • Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Abe, Miou
  • Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Aoshima, Keisuke
  • Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Kobayashi, Atsushi
  • Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Fukushi, Hideto
  • Laboratory of Veterinary Microbiology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Kimura, Takashi
  • Laboratory of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

MeSH Terms

  • Herpesvirus 1, Equid / genetics
  • Animals
  • Promoter Regions, Genetic
  • Horses
  • Genes, Immediate-Early
  • Gene Expression Regulation, Viral
  • Cell Line
  • Virus Replication
  • RNA, Antisense / genetics
  • Transcription, Genetic
  • Herpesviridae Infections / veterinary
  • Herpesviridae Infections / virology
  • Horse Diseases / virology
  • Open Reading Frames

Grant Funding

  • 22H02508 / Ministry of Education, Culture, Sports, Science, and Technology, Japan

Conflict of Interest Statement

The authors declare no conflicts of interest.

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