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Home / Equine Research Bank / BMC Vet Res / Identifying 3rd larval stages of common strongylid and non-s...
BMC veterinary research2022; 18(1); 432; doi: 10.1186/s12917-022-03526-8

Identifying 3rd larval stages of common strongylid and non-strongylid nematodes (class: Nematoda) infecting Egyptian equines based on morphometric analysis.

Abstract: Strongylid and non-strongylid nematodes are one of the most important parasites infecting equines. The traditional method to identify these nematodes is through coproscopy and fecal culture. Because of the scarcity of data published in Egypt discussing the morphometric features of infective 3 larvae of these nematodes, this study aims to provide a morphometric key for L3 of common strongylid and non-strongylid nematodes infecting Egyptian equines. For this reason, we cultured fecal samples containing GINs eggs and 3 larval stages were identified based on their morphology (i.e., shape and number of intestinal cells (IC), shape of the esophagus, and shape of the tail sheath) in addition to computing their dimensions (i.e., length of larvae with sheath, length of the esophagus, length of intestinal cells, and body breadth). We identified 3 larval stages of four strongylid nematodes (Cyathostomum sensu lato, Strongylus vulgaris, Strongylus equinus, and Strongylus edentatus) as well as two non-strongylid nematodes (Strongyloides westeri, and Trichostrongylus axei). Statistically, our results revealed significant differences in terms of total length, body width, esophagus length, and gut length among 3 larvae identified in the current study. The combination of both morphological and metric keys will allow the better identification of common strongylid and non-strongylid nematodes infecting equines.
© 2022. The Author(s).
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Publication Date: 2022-12-12 PubMed ID: 36503529PubMed Central: PMC9743504DOI: 10.1186/s12917-022-03526-8Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is focused on identifying the 3rd larval stages of common strongylid and non-strongylid nematodes, two critical parasites infecting horses in Egypt, using morphometric analysis.

Objective of the Study

  • The primary goal of this study was to gather detailed morphometric information about the 3rd life stages of common strongylid and non-strongylid nematodes infecting equines in Egypt.

Methodology

  • The researchers cultured fecal samples that contained Gastrointestinal Nematodes (GINs) eggs.
  • They used morphological features such as the shape and number of intestinal cells, the shape of the esophagus, and the shape of the tail sheath, along with their dimensions, to identify 3rd larval stages of the various nematodes.
  • The metrics computed included the length of larvae with sheath, length of the esophagus, the length of intestinal cells, and body breadth.

Findings

  • Through their practices, they identified the 3rd larval stages of four strongylid nematodes (Cyathostomum sensu lato, Strongylus vulgaris, Strongylus equinus, Strongylus edentatus) and two non-strongylid nematodes (Strongyloides westeri, Trichostrongylus axei).
  • The results showed significant variations in the total length, body width, esophagus length, and gut length among the 3rd larval stages of the different nematodes they identified in the study.

Conclusion

  • The study posits that the combination of morphological and metric keys can improve the identification of common strongylid and non-strongylid nematodes that infect equines. This finding can help more successfully address equine nematode infections by facilitating accurate identification and, thus, treatment of these nematode infections.

Cite This Article

APA
Amer MM, Desouky AY, Helmy NM, Abdou AM, Sorour SS. (2022). Identifying 3rd larval stages of common strongylid and non-strongylid nematodes (class: Nematoda) infecting Egyptian equines based on morphometric analysis. BMC Vet Res, 18(1), 432. https://doi.org/10.1186/s12917-022-03526-8

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 18
Issue: 1
Pages: 432
PII: 432

Researcher Affiliations

Amer, Moaz M
  • Biotechnology Department, Animal Health Research Institute, Dokki, 12618, Egypt. mooaz.amer@gmail.com.
Desouky, A Y
  • Parasitology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt.
Helmy, Nashwa M
  • Biotechnology Department, Animal Health Research Institute, Dokki, 12618, Egypt.
Abdou, Ahmed M
  • Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Sorour, Sh S
  • Parasitology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt.

MeSH Terms

  • Animals
  • Horses
  • Larva
  • Ovum
  • Nematoda
  • Strongylus
  • Strongyloidea

Conflict of Interest Statement

The authors declare to have no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Elghandour MMMY, Maggiolino A, Vázquez-Mendoza P, Alvarado-Ramírez ER, Cedillo-Monroy J, De Palo P, Salem AZM. Moringa oleifera as a Natural Alternative for the Control of Gastrointestinal Parasites in Equines: A Review.. Plants (Basel) 2023 May 8;12(9).
    doi: 10.3390/plants12091921pubmed: 37176979google scholar: lookup
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