IgG antibody subclass response against equine herpesvirus type 4 in horses.
Abstract: In this study, IgG subclass responses against equine herpesvirus type 4 (EHV-4) were examined by enzyme-linked immunosorbent assay (ELISA) using a type-specific region of EHV-4 glycoprotein G (gG). ELISA using sera collected from horses experimentally infected with EHV-4 revealed that IgGa and IgGb antibodies were detected at high level, but IgGc and IgG(T) antibody responses were detected at low level or were undetectable. The IgGa antibody response reached its peak on day 10 post-infection, and then dropped. The IgGb antibody response reached its maximum level on day 12 post-infection, and then the level was sustained during at least 28 days after infection. Forty healthy racehorses that had already been infected with EHV-4 possessed antibody against EHV-4. Although IgGa antibodies specific for EHV-4 were not detected in any horses, IgGb antibodies were detected and the levels correlated with total IgG antibodies against EHV-4 gG. The results suggest that EHV-4-specific IgGa and IgGb antibodies are induced in EHV-4-infected horses, and that IgGb antibody, but not IgGa, is long lasting.
Publication Date: 2002-06-29 PubMed ID: 12088649DOI: 10.1016/s0165-2427(02)00130-7Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article reveals a study on the response of the IgG antibody subclass against equine herpesvirus type 4 (EHV-4) in horses. The researchers discovered that two types of IgG antibodies, IgGa and IgGb, were produced at high levels following an EHV-4 infection, while the other antibody types, IgGc and IgG(T), were either lower in number or undetectable.
Experimentation and Finding
- The experimentation made use of an enzyme-linked immunosorbent assay (ELISA) coupled with a portion of the EHV-4 specific glycoprotein G to study the reaction of the IgG antibody subclasses.
- Results showed that, among horses that were infected with EHV-4, IgGa and IgGb antibodies were observed at high levels. However, IgGc and IgG(T) levels were lower or not detected at all.
- The IgGa antibody was observed to peak on the tenth day following infection, after which it declined. On the other hand, IgGb antibody levels reached their highest on the twelfth day after infection and then stayed constant for at least 28 days.
Correlation with Healthy Racehorses
- Examination of the sera from forty healthy racehorses that had previously had an EHV-4 infection revealed the presence of EHV-4-specific antibodies.
- None of the horses demonstrated IgGa antibodies specific to EHV-4, though IgGb antibodies were noticed and their levels corresponded with the overall IgG antibodies found against EHV-4 glycoprotein G.
Conclusions
- The results indicated that EHV-4 infection in horses leads to the induction of EHV-4-specific IgGa and IgGb antibodies.
- IgGa antibodies appear to be short-lived, while IgGb antibodies demonstrate a persistent immunity.
Cite This Article
APA
Mizukoshi F, Maeda K, Hamano M, Iwata H, Matsumura T, Kondo T, Sugiura T.
(2002).
IgG antibody subclass response against equine herpesvirus type 4 in horses.
Vet Immunol Immunopathol, 88(1-2), 97-101.
https://doi.org/10.1016/s0165-2427(02)00130-7 Publication
Researcher Affiliations
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
MeSH Terms
- Animals
- Antibodies, Viral / biosynthesis
- Antibodies, Viral / blood
- Antibodies, Viral / immunology
- Enzyme-Linked Immunosorbent Assay / veterinary
- Herpesviridae Infections / immunology
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Herpesvirus 4, Equid / immunology
- Horse Diseases / immunology
- Horse Diseases / virology
- Horses
- Immunoglobulin G / biosynthesis
- Immunoglobulin G / blood
- Immunoglobulin G / immunology
- Immunoglobulin Isotypes / immunology
Citations
This article has been cited 4 times.- Thorsteinsdóttir L, Jónsdóttir S, Stefánsdóttir SB, Andrésdóttir V, Wagner B, Marti E, Torsteinsdóttir S, Svansson V. The effect of maternal immunity on the equine gammaherpesvirus type 2 and 5 viral load and antibody response.. PLoS One 2019;14(6):e0218576.
- Cunha CW, McGuire TC, Kappmeyer LS, Hines SA, Lopez AM, Dellagostin OA, Knowles DP. Development of specific immunoglobulin Ga (IgGa) and IgGb antibodies correlates with control of parasitemia in Babesia equi Infection.. Clin Vaccine Immunol 2006 Feb;13(2):297-300.
- Maeda K, Mizukoshi F, Hamano M, Kai K, Kondo T, Matsumura T. Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G.. Clin Diagn Lab Immunol 2005 Jan;12(1):122-4.
- Maeda K, Mizukoshi F, Hamano M, Kai K, Iwata H, Kondo T, Matsumura T. Development of an equine herpesvirus type 4-specific enzyme-linked immunosorbent assay using a B-cell epitope as an antigen.. J Clin Microbiol 2004 Mar;42(3):1095-8.
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