Immunity-related gene single nucleotide polymorphisms associated with Rhodococcus equi infection in foals.
Abstract: In previous work, we found significant associations of horse polymorphic microsatellite and immunity-related (IR) gene markers with Rhodococcus equi infection of foals. Here, a statistically significant association between a single nucleotide polymorphism (SNP) within the interleukin 7 receptor-encoding gene (IL7R) with high R. equi burden in transtracheal aspirates was found (Fisher's F = 0.043, odds ratio: 8.00, 95% confidence interval: 1.127-56.795). Further positional and/or functional candidate genes investigated TLR2, IL13, IL17A, IL28R, TACE/ADAM 17 and GBP1, were not associated with infection in this study. SNPs analysed were found by sequencing and appropriate restriction fragment length polymorphism markers were developed. Their associations with R. equi infection were tested by genotyping thoroughbred foals from the original study. The association was confirmed by analysing genotypes composed with genes previously reported to be associated with R. equi infection in the same group.
Publication Date: 2009-12-03 PubMed ID: 20002811DOI: 10.1111/j.1744-313X.2009.00890.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article explores the connection between a specific genetic variation, a single nucleotide polymorphism (SNP), in horses and their susceptibility to Rhodococcus equi infection.
Significance of the Study
- The study is important because it examines the relationship between specific genetic variations, called single nucleotide polymorphisms (SNPs), in horses and their vulnerability to an infectious disease called Rhodococcus equi. This bacterial infection primarily affects foals and is prevalent in horses worldwide. Understanding this relationship could lead to improved preventative measures and treatments.
Key Findings
- The main discovery was a significant correlation between an SNP in the interleukin 7 receptor-encoding gene (IL7R) and a high burden of R. equi bacteria in the lungs of infected foals. This was determined by comparing the SNP’s presence in a group of infected thoroughbred foals from the original study.
- The researchers also tested a few other candidate genes (TLR2, IL13, IL17A, IL28R, TACE/ADAM 17 and GBP1), but found no significant connection between their SNPs and infection susceptibility.
Methods Used
- Researchers initially found the SNPs by sequencing the candidate genes and then used restriction fragment length polymorphism markers for detection.
- They then compared the known genes associated with R. equi infection with the genetic profiles (genotypes) of the thoroughbred foals from the original study to confirm this association.
Implication of the Findings
- The identified SNP in the IL7R gene could be used as a potential genetic marker for R. equi infection vulnerability. This could prove particularly vital in breeding programs, as it could enable breeders to select for resistance to this common and often fatal foal disease.
- By knowing which genetic variations increase susceptibility to R. equi, it could lead to better diagnostic tools, therapeutic strategies and preventative measures for the disease.
Cite This Article
APA
Horin P, Sabakova K, Futas J, Vychodilova L, Necesankova M.
(2009).
Immunity-related gene single nucleotide polymorphisms associated with Rhodococcus equi infection in foals.
Int J Immunogenet, 37(2), 67-71.
https://doi.org/10.1111/j.1744-313X.2009.00890.x Publication
Researcher Affiliations
- Institute of Animal Genetics, Faculty of Veterinary Medicine, Brno 612 42, Czech Republic. horinp@vfu.cz
MeSH Terms
- Actinomycetales Infections / genetics
- Actinomycetales Infections / microbiology
- Actinomycetales Infections / veterinary
- Animals
- Gene Frequency
- Genetic Predisposition to Disease / genetics
- Genotype
- Horse Diseases / genetics
- Horse Diseases / immunology
- Horse Diseases / microbiology
- Horses
- Immunity / genetics
- Polymorphism, Restriction Fragment Length
- Polymorphism, Single Nucleotide
- Receptors, Interleukin-7 / genetics
- Rhodococcus equi / immunology
- Rhodococcus equi / isolation & purification
- Sequence Analysis, DNA
- Trachea / microbiology
Citations
This article has been cited 3 times.- McQueen CM, Whitfield-Cargile CM, Konganti K, Blodgett GP, Dindot SV, Cohen ND. TRPM2 SNP genotype previously associated with susceptibility to Rhodococcus equi pneumonia in Quarter Horse foals displays differential gene expression identified using RNA-Seq.. BMC Genomics 2016 Dec 5;17(1):993.
- McQueen CM, Dindot SV, Foster MJ, Cohen ND. Genetic Susceptibility to Rhodococcus equi.. J Vet Intern Med 2015 Nov-Dec;29(6):1648-59.
- McQueen CM, Doan R, Dindot SV, Bourquin JR, Zlatev ZZ, Chaffin MK, Blodgett GP, Ivanov I, Cohen ND. Identification of genomic loci associated with Rhodococcus equi susceptibility in foals.. PLoS One 2014;9(6):e98710.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
