Immunochemical studies of infectious mononucleosis–XI. comparison of heterophile antibody inhibitors from the erythrocyte membranes of four mammalian species.
Abstract: Immunochemical comparisons were made of the reactivity of membrane glycoproteins from horse, bovine, sheep and goat erythrocytes with heterophile antibodies of infectious mononucleosis. The four receptors were tested as competitive inhibitors of a sandwich-type solid-phase radioimmunoassay and of agglutination of glycoprotein-latex reagents by infectious mononucleosis serum. The results of this study showed that the bovine glycoprotein had a superior reactivity with this heterophile antibody system and sheep erythrocyte glycoprotein was the least reactive. The latter had negligible ability to displace 125I-bovine glycoprotein and was a very poor inhibitor of the agglutination of a bovine glycoprotein-latex reagent by infectious mononucleosis serum. Horse and goat glycoproteins were more efficient inhibitors than sheep glycoprotein but less active than the preparation from bovine red cells. All of the inhibitory activity of sheep, horse and goat glycoproteins, and a major portion of that of the bovine glycoprotein was destroyed by neuraminidase treatment. We have termed this receptor--shared by all four species--the Paul-Bunnell receptor, since by definition Paul-Bunnell antibody is a sheep erythrocyte agglutinin which is also reactive with horse, bovine and goat erythrocytes. The neuraminidase (and alkaline borohydride) resistant receptor of bovine glycoprotein has been designated the Bo receptor because it is not common to the other three species.
Publication Date: 1983-01-01 PubMed ID: 6406839DOI: 10.1016/0161-5890(83)90099-8Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research compares the interaction between membrane glycoproteins from various mammals and heterophile antibodies of infectious mononucleosis. Researchers found that bovine glycoprotein showed the highest reactivity with these antibodies, and sheep erythrocyte glycoprotein indicated the lowest reactivity.
Objective of the Research
- The study was conducted with the aim to compare the immunochemical reactivity of membrane glycoproteins, from horse, bovine, sheep and goat erythrocytes, with the heterophile antibodies of infectious mononucleosis.
Methodology
- The researchers utilized four different types of glycoproteins and studied their inhibitory capacity in a sandwich-type solid-phase radioimmunoassay.
- The agglutination of glycoprotein-latex reagents by infectious mononucleosis serum was also studied.
Key Findings
- The results revealed that the bovine glycoprotein exhibits the best reactivity with the heterophile antibody system. Sheep erythrocyte glycoprotein was found to possess the lowest reactivity, with minimal ability to displace 125I-bovine glycoprotein.
- Sheep glycoprotein also demonstrated poor inhibitory powers in the agglutination of a bovine glycoprotein-latex reagent by the serum of the infectious mononucleosis.
- Horse and goat glycoproteins posed a better inhibitory activity than sheep glycoprotein, yet they were less active compared to bovine glycoproteins.
- All of the inhibitory activity of sheep, horse and goat glycoproteins, along with a major part of the bovine glycoprotein, was destroyed after treatment with neuraminidase.
Research Terminology
- The universal receptor, which was discovered in all the four species, was termed as the Paul-Bunnell receptor. This was named so due to the relationship with the Paul-Bunnell antibody, which is a sheep erythrocyte agglutinin that can also react with horse, bovine and goat erythrocytes.
- The research distinguished a neuraminidase (and alkaline borohydride) resistant receptor in bovine glycoprotein, which they named as the Bo receptor owing to its presence only in bovines and not common to the other three species.
Cite This Article
APA
Latif ZA, Fletcher MA.
(1983).
Immunochemical studies of infectious mononucleosis–XI. comparison of heterophile antibody inhibitors from the erythrocyte membranes of four mammalian species.
Mol Immunol, 20(1), 1-10.
https://doi.org/10.1016/0161-5890(83)90099-8 Publication
Researcher Affiliations
MeSH Terms
- Agglutination Tests
- Animals
- Antibodies, Heterophile / immunology
- Antibodies, Viral / immunology
- Binding, Competitive
- Cattle
- Erythrocyte Membrane / immunology
- Erythrocytes / immunology
- Glycoproteins / immunology
- Goats
- Horses
- Humans
- Immunodiffusion
- Infectious Mononucleosis / immunology
- Radioimmunoassay
- Sheep
Grant Funding
- AM16763 / NIADDK NIH HHS
Citations
This article has been cited 5 times.- Mishra B, Mohan B, Ratho RK. Heterophile antibody positive infectious mononucleosis.. Indian J Pediatr 2004 Jan;71(1):15-8.
- Rogers R, Windust A, Gregory J. Evaluation of a novel dry latex preparation for demonstration of infectious mononucleosis heterophile antibody in comparison with three established tests.. J Clin Microbiol 1999 Jan;37(1):95-8.
- Svahn A, Magnusson M, Jägdahl L, Schloss L, Kahlmeter G, Linde A. Evaluation of three commercial enzyme-linked immunosorbent assays and two latex agglutination assays for diagnosis of primary Epstein-Barr virus infection.. J Clin Microbiol 1997 Nov;35(11):2728-32.
- Fletcher MA, Klimas NG, Latif ZA, Caldwell KE. Serodiagnosis of infectious mononucleosis with a bovine erythrocyte glycoprotein.. J Clin Microbiol 1983 Sep;18(3):495-9.
- Atrah HI, Davidson RJ. Mechanism of action of intravenous immunoglobulin in immune-mediated cytopenias.. J Clin Pathol 1988 Dec;41(12):1249-55.
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