Immunocytochemical demonstration of calcitonin-containing C-cells in the thyroid glands of different mammals.
Abstract: In the thyroid glands of the horse, pig, deer, mole, and rat, C-cells could be demonstrated by means of the immunocytochemical PAP-technique using rabbit antisera against human calcitonin. Only in ruminants, the cross-reaction between the intracellularly stored antigen and the antibodies used appeared to be incomplete.
Publication Date: 1978-01-31 PubMed ID: 342107DOI: 10.1007/BF00224943Google Scholar: Lookup
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- Journal Article
Summary
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The research article explores how C-cells containing calcitonin can be identified in the thyroid glands of various mammals using a specific immunocytochemical technique called PAP. However, the method wasn’t completely successful in all tested animals, showing some limitations when applied to ruminants.
Research Methodology
The study made use of a technique known as the PAP (peroxidase-antiperoxidase) method. This is an immunocytochemical technique generally employed to detect specific proteins in cells. It involves the following steps:
- The gland tissues from the studied organisms (horse, pig, deer, mole, and rat) were treated with a special reagent known as “rabbit antisera against human calcitonin”. This reagent helps to reveal where the calcitonin proteins are located within the cells.
- The reaction between the rabbit antisera and the calcitonin stored in the cells of the thyroid gland was then observed. If the reaction is successful, this would suggest that the cell is indeed a C-cell, a specialized cell type found within the thyroid gland that is known to produce calcitonin.
Findings
The research found that for most mammals (like the horse, pig, deer, mole, and rat), the immunocytochemical technique was successful in revealing the presence of C-cells in the thyroid gland.
- These cells were shown to contain calcitonin, a critical hormone that helps to regulate calcium and phosphate levels in the body.
- Therefore, in these species, the technique could reliably demonstrate the presence of C-cells by reacting with the calcitonin stored within these cells.
Limitations
However, the study also revealed some limitations of the PAP-technique:
- Specifically, in ruminants (a category of mammals that includes animals like cows and sheep), the cross-reaction between the antisera and the intracellular calcitonin seemed to be incomplete.
- This suggests that the technique may not be 100% reliable in these species, and there could be factors in ruminants that alter the effectiveness of the method.
In summary, the research demonstrates that immunocytochemical PAP-technique is generally successful in identifying C-cells in the thyroid gland of various mammals, but the method may have less efficacy in ruminants. More study may be needed to understand and overcome these limitations.
Cite This Article
APA
Blähser S.
(1978).
Immunocytochemical demonstration of calcitonin-containing C-cells in the thyroid glands of different mammals.
Cell Tissue Res, 186(3), 551-558.
https://doi.org/10.1007/BF00224943 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antigen-Antibody Reactions
- Calcitonin / analysis
- Deer
- Horses
- Humans
- Immunoenzyme Techniques
- Moles
- Rabbits / immunology
- Rats
- Swine
- Thyroid Gland / cytology
References
This article includes 6 references
- Vandesande F, Dierickx K. Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretroy system of the rat.. Cell Tissue Res 1975 Dec 2;164(2):153-62.
- Pearse AG. Common cytochemical properties of cells producing polypeptide hormones, with particular reference to calcitonin and the thyroid C cells.. Vet Rec 1966 Nov 19;79(21):587-90.
- Vandesande F, DeMey J, Dierickx K. Identification of neurophysin producing cells. I. The origin of the neurophysin-like substance-containing nerve fibres of the external region of the median eminence of the rat.. Cell Tissue Res 1974;151(2):187-200.
- Blähser S, Kraus G. [A method for the demonstration of the histological functional stages of thyreocytes and calcitonin cells in the thyroid of the rat].. Mikroskopie 1972;28(9):288-91.
- Pearse AG. The cytochemistry of the thyroid C cells and their relationship to calcitonin.. Proc R Soc Lond B Biol Sci 1966 Apr 19;164(996):478-87.
- Solcia E, Vassallo G, Capella C. Selective staining of endocrine cells by basic dyes after acid hydrolysis.. Stain Technol 1968 Sep;43(5):257-63.
Citations
This article has been cited 6 times.- Sokołowska J, Cywińska A, Puchalska M. Comparative Histology of C Thyrocytes in Four Domestic Animal Species: Dog, Pig, Horse, and Cattle.. Animals (Basel) 2022 May 23;12(10).
- Blähser S, Dubois MP. Immunocytochemical demonstration of met-enkephalin in the central nervous system of the domestic fowl.. Cell Tissue Res 1980;213(1):53-68.
- Guilloteau D, Fetissof F, Renjard L, Dubois MP, Besnard JC. Immunocytological study of the distribution of C-cells calcitonin in the thyroid gland of the normal adult gerbil.. Experientia 1983 Aug 15;39(8):876-8.
- Bläsher S, Heinrichs M. Immunoreactive neuropeptide systems in avian embryos (domestic mallard, domestic fowl, Japanese quail).. Cell Tissue Res 1982;223(2):287-303.
- Blähser S, Fellmann D, Bugnon C. Immunocytochemical demonstration of somatostatin-containing neurons in the hypothalamus of the domestic mallard.. Cell Tissue Res 1978 Dec 14;195(1):183-7.
- Treilhou-Lahille F, Cressent M, Taboulet J, Moukhtar MS. Immunohistochemical staining of mouse C cells during post-natal histogenesis of the thyroid.. Histochemistry 1979 Sep;63(1):69-80.
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