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Home / Equine Research Bank / Infect Immun / Immunodiffusion studies of purified equine infectious anemia...
Infection and immunity1971; 3(3); 373-377; doi: 10.1128/iai.3.3.373-377.1971

Immunodiffusion studies of purified equine infectious anemia virus.

Abstract: Antigenicity of purified equine infectious anemia (EIA) virus was examined by immunodiffusion against sera obtained from horses experimentally infected with EIA virus. The purified virus reacted with the infected horse serum, and virus-specific precipitating antibody was demonstrated. Furthermore, it was found that purified EIA virus reacted against the serum of horses infected with all strains of EIA virus which were antigenically different from one another. From the result, group-specific components of the virus rather than strain-specific ones were considered to be involved in the reaction. Serological reactivity was lost by adding antiserum from the infected horse to the antigen. The precipitating antibody usually appeared in the serum 1 to 2 weeks after the first febrile attack of EIA and remained for a longer period. Some characteristics of the purified antigen and specificity of the reaction for EIA are described.
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Publication Date: 1971-03-01 PubMed ID: 16557982PubMed Central: PMC416160DOI: 10.1128/iai.3.3.373-377.1971Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the study of the antigenic properties of the purified equine infectious anemia (EIA) virus, using immunodiffusion against serum obtained from horses that have been infected with the EIA virus experimentally.

Objective of the Study

  • The primary purpose of this research is to explore the antigenic characteristics of the purified EIA virus by applying an immunodiffusion test against sera taken from experimentally EIA-infected horses.

Methodology and Process

  • The researchers first purified the EIA virus. This purified virus was then reacted with the serum from horses that were experimentally infected with the EIA virus.
  • The reaction demonstrated a virus-specific precipitating antibody, confirming the presence of the virus.
  • The study further discovered that the purified EIA virus reacted against the serum of horses infected with all strains of the EIA virus. This indicated that these strains, although antigenically different from one another, share some group-specific components.
  • This led the researchers to conclude that the group-specific components of the virus, rather than strain-specific ones, are involved in the reaction.
  • When an antiserum from an infected horse was added to the antigen, the serological reactivity was lost, indicating that the serum has an effect on the antigen.

Findings and Timeline

  • The precipitating antibody usually appears in the serum 1 to 2 weeks following the patient’s first fever attack from EIA.
  • The antibody remains in the serum for an extended period.
  • Finally, the characteristics of the purified antigen, as well as the specificity of the reaction for EIA, are discussed in the research paper.

Cite This Article

APA
Nakajima H, Ushimi C. (1971). Immunodiffusion studies of purified equine infectious anemia virus. Infect Immun, 3(3), 373-377. https://doi.org/10.1128/iai.3.3.373-377.1971

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 3
Issue: 3
Pages: 373-377

Researcher Affiliations

Nakajima, H
  • Equine Infectious Anemia Research Division, National Institute of Animal Health, Kodaira, Tokyo, Japan.
Ushimi, C

    References

    This article includes 12 references
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    10. Coggins L, Norcross NL. Immunodiffusion reaction in equine infectious anemia.. Cornell Vet 1970 Apr;60(2):330-5.
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      pubmed: 4292232

    Citations

    This article has been cited 12 times.
    1. dos Reis JK, Melo LM, Rezende MR, Leite RC. Use of an ELISA test in the eradication of an equine infectious anaemia focus.. Trop Anim Health Prod 1994 May;26(2):65-8.
      doi: 10.1007/BF02239899pubmed: 7941030google scholar: lookup
    2. Sugiura T, Nakajima H. Indirect hemagglutination test in equine infectious anemia.. Can J Comp Med 1982 Jan;46(1):60-4.
      pubmed: 6280821
    3. Sentsui H, Kono Y. Hemagglutination of several strains of equine infectious anemia virus.. Arch Virol 1981;67(1):75-84.
      doi: 10.1007/BF01314604pubmed: 6263225google scholar: lookup
    4. Norcross NL, Coggins L. Characterization of an equine infectious anemia antigen extracted from infected horse spleen tissue.. Infect Immun 1971 Nov;4(5):528-31.
      doi: 10.1128/iai.4.5.528-531.1971pubmed: 5005308google scholar: lookup
    5. Nakajima H, Norcross NL, Coggins L. Demonstration of antigenic identity between purified equine infectious anemia virus and an antigen extracted from infected horse spleen.. Infect Immun 1972 Sep;6(3):416-7.
      doi: 10.1128/iai.6.3.416-417.1972pubmed: 4629262google scholar: lookup
    6. Nakajima H, Yoshino T, Ushimi C. Equine infectious anemia virus from infected horse serum.. Infect Immun 1974 Sep;10(3):667-8.
      doi: 10.1128/iai.10.3.667-668.1974pubmed: 4372175google scholar: lookup
    7. Nakajima H, Ushimi C, Fukunaga Y, Hirasawa K. Preparation of equine infectious anemia virus antigen for immunodiffusion test.. Arch Gesamte Virusforsch 1973;42(4):339-45.
      doi: 10.1007/BF01250714pubmed: 4358258google scholar: lookup
    8. Ushimi C, Henson JB, Gorham JR. Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence.. Infect Immun 1972 Jun;5(6):890-5.
      doi: 10.1128/iai.5.6.890-895.1972pubmed: 4344094google scholar: lookup
    9. Kono Y, Kobayashi K, Fukunaga Y. Antigenic drift of equine infectious anemia virus in chronically infected horses.. Arch Gesamte Virusforsch 1973;41(1):1-10.
      doi: 10.1007/BF01249923pubmed: 4123810google scholar: lookup
    10. Matheka HD, Coggins L, Shively JN, Norcross NL. Purification and characterization of equine infectious anemia virus.. Arch Virol 1976;51(1-2):107-14.
      doi: 10.1007/BF01317839pubmed: 183628google scholar: lookup
    11. Sugiura T, Nakajima H. Purification of equine infectious anemia virus antigen by affinity chromatography.. J Clin Microbiol 1977 Jun;5(6):635-9.
      doi: 10.1128/jcm.5.6.635-639.1977pubmed: 69631google scholar: lookup
    12. Sentsui H, Kono Y. Hemagglutination by equine infectious anemia virus.. Infect Immun 1976 Aug;14(2):325-31.
      doi: 10.1128/iai.14.2.325-331.1976pubmed: 9361google scholar: lookup
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