Immunohistochemical characterization of estrogen and progesterone receptors in lymphoma of horses.
Abstract: Immunohistochemical techniques were used to examine 29 cases of equine lymphoma for estrogen receptor (ER) and progesterone receptor (PR) expression. The lymphomas examined included T-cell-rich large B-cell lymphomas, B-cell neoplasms, and T-cell lymphomas. The individual cases were also classified according to the anatomic location of the tumors. One normal equine lymph node was also examined for ER and PR expression. All of the cases of equine lymphoma and the normal lymph node were negative for Er. A total of 16/29 (55%) PR-positive lymphomas were identified. Seven of the 12 (58%) T-cell-rich large B-cell lymphomas were positive, 7/11 (64%) B-cell tumors were positive, and 2/6 (33%) T-cell neoplasms were positive. Anatomically, 6/9 (66%) subcutaneous lymphomas were PR positive, 3/5 (60%) intrathoracic lymphomas were positive, 1/4 (25%) intra-abdominal lymphomas were positive, 2/5 (40%) intra-abdominal/intrathoracic lymphomas were positive, 1/2 (50%) upper airway lymphomas were positive, and 3/3 (100%) splenic lymphomas were positive. One case involving abdominal and thoracic tumors and leukemia was negative for PR expression. The normal lymph node contained a low percentage (1.9%) of PR-positive lymphocytes. The presence of PR in neoplastic equine lymphoid tissue indicates that these tumors may be responsive to serum progesterone. Also, identification of PR-positive cells in the normal lymph node suggests that PR may be constitutively expressed in normal equine lymphocytes. Further studies are needed to quantify PR levels in normal and malignant equine lymphoid tissue and to determine the usefulness of either progestin or antiprogestin drugs in the management of equine lymphoma.
Publication Date: 2002-06-19 PubMed ID: 12070809DOI: 10.1111/j.1939-165x.2000.tb00396.xGoogle Scholar: Lookup
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Summary
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This research paper investigates the presence of estrogen and progesterone receptors in various types of lymphoma found in horses, finding that all lymphoma cases were negative for estrogen receptors while more than half showed progesterone receptor expression, suggesting hormonal therapy might be a future treatment route for equine lymphoma.
Immunohistochemical Methods and Sample Types
- The study applied immunohistochemical techniques to study 29 instances of equine lymphoma. These techniques entail using antibodies to detect specific proteins in cells of a tissue section, in this case, estrogen (ER) and progesterone receptors (PR).
- The different types of lymphomas studied included T-cell-rich large B-cell lymphomas, B-cell neoplasms, and T-cell lymphomas.
- Additionally, each case was classified according to the anatomic location of the tumors, such as subcutaneous, intrathoracic, intra-abdominal, and more.
- The researchers also included a normal equine lymph node for comparison.
Findings: Estrogen and Progesterone Receptor Expression
- The researchers found all the lymphoma cases and the normal lymph node to be negative for ER, indicating that these forms of cancer in horses do not respond to estrogen.
- However, 55% or 16 out of the 29 lymphoma samples were found to be PR-positive, expressing PR to varying degrees depending on the type and location of the tumor.
- The normal lymph node showed a slight PR-positive expression, implying that PR may be consistently expressed in normal equine lymphocytes, albeit at a lower level compared to the cancerous tissues.
Implications and Future Directions
- The presence of PR in equine lymphoid cancer tissue indicates that these tumors might react to serum progesterone, leading the researchers to theorize that hormonal therapy, either with progestin or antiprogestin drugs, could potentially be useful in equine lymphoma treatment.
- Further research is necessary to validate this theory, quantify PR levels in normal and malignant equine lymphoid tissue, and assess the effectiveness of hormonal drugs in managing equine lymphoma.
Cite This Article
APA
Henson KL, Alleman AR, Kelley LC, Mahaffey EA.
(2002).
Immunohistochemical characterization of estrogen and progesterone receptors in lymphoma of horses.
Vet Clin Pathol, 29(2), 40-46.
https://doi.org/10.1111/j.1939-165x.2000.tb00396.x Publication
Researcher Affiliations
- Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA. allemanr@mail.vetmed.ufl.edu
Citations
This article has been cited 3 times.- Gao DL, Fu QQ, Zhang TT, Sun L, Pan Y, Zhai QL. Occurrence of lymphoma in non-gonadal organ during pregnancy: a report on four cases and literature review. Cancer Biol Med 2016 Sep;13(3):399-403.
- Doyle AJ, MacDonald VS, Bourque A. Use of lomustine (CCNU) in a case of cutaneous equine lymphoma. Can Vet J 2013 Dec;54(12):1137-41.
- Canisso IF, Pinn TL, Gerdin JA, Ollivett TL, Buckles EL, Schweizer CM, Ainsworth DM. B-cell multicentric lymphoma as a probable cause of abortion in a Quarter horse broodmare. Can Vet J 2013 Mar;54(3):288-91.
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