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Immunohistochemical determination of the expression of endothelin receptors in bronchial smooth muscle and epithelium of healthy horses and horses affected by summer pasture-associated obstructive pulmonary disease.
Abstract: To immunohistochemically determine the expression of endothelin (ET) receptors in bronchial smooth muscle and epithelium of healthy horses and horses affected by summer pasture-associated obstructive pulmonary disease (SPAOPD). Methods: Tissue specimens obtained from 8 healthy and 8 SPAOPD-affected horses. Methods: Horses were examined and assigned to healthy and SPAOPD groups. Horses were then euthanatized, and tissue specimens containing bronchi of approximately 4 to 8 mm in diameter were immediately collected from all lung lobes, fixed in zinc-formalin solution for 12 hours, and embedded in paraffin. Polyclonal primary antibodies against ET-A or ET-B receptors at a dilution of 1:200 and biotinylated IgG secondary antibodies were applied to tissue sections, followed by the addition of an avidin-biotin immunoperoxidase complex. Photographs of the stained slides were digitally recorded and analyzed by use of image analysis software to determine the intensity of staining. Two-way ANOVA was used for statistical analysis. Results: The left diaphragmatic lung lobe of SPAOPD-affected horses had a significantly greater area of bronchial smooth muscle that immunostained for ET-A, compared with that for healthy horses. All lung lobes of SPAOPD-affected horses, except for the right diaphragmatic lobe, had significantly greater staining for ET-B receptors in bronchial smooth muscle, compared with results for healthy horses. Conclusions: This study revealed overexpression of ET-A and, in particular, ETB receptors in the bronchial smooth muscle of SPAOPD-affected horses, which suggested upregulation of these receptors. These findings improve our understanding of the role of ET-1 in the pathogenesis of SPAOPD.
Publication Date: 2006-02-04 PubMed ID: 16454644DOI: 10.2460/ajvr.67.2.348Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research article is about examining the expression of endothelin (ET) receptors in the bronchial smooth muscle and epithelium of both healthy horses and those affected by summer pasture-associated obstructive pulmonary disease (SPAOPD). The results revealed an overexpression of ET receptors in SPAOPD-affected horses.
Methodology
- The study included tissue samples from 8 healthy and 8 SPAOPD-affected horses.
- Horses were examined and assigned to either the healthy or SPAOPD groups.
- The horses were then euthanatized, and tissue samples containing bronchi around 4 to 8 mm in diameter were immediately collected from all lung lobes.
- These tissue specimens were fixed in a zinc-formalin solution for 12 hours, then embedded in paraffin.
- Focusing on identifying ET-A and ET-B receptors, polyclonal primary antibodies were applied to the tissue sections, followed by biotinylated IgG secondary antibodies.
- Resulting stained slides were photographed, then digitally analyzed using image analysis software to determine staining intensity.
- A two-way ANOVA was used for statistical analysis.
Results
- The left diaphragmatic lung lobe of SPAOPD-affected horses showed a significantly greater area of bronchial smooth muscle that stained for ET-A receptors, compared to healthy horses.
- All lung lobes of SPAOPD-affected horses, excluding the right diaphragmatic lobe, showed significantly greater staining for ET-B receptors in bronchial smooth muscle, compared to healthy horses.
Conclusion
- The findings revealed a notable overexpression of ET-A and particularly ET-B receptors in the bronchial smooth muscle of SPAOPD-affected horses. This suggested that there might be an upregulation of these receptors in horses with SPAOPD.
- This study contributes to our understanding about the role of ET-1 (endothelin-1) in the development of summer pasture-associated obstructive pulmonary disease in horses.
Cite This Article
APA
Polikepahad S, Paulsen DB, Moore RM, Costa LR, Venugopal CS.
(2006).
Immunohistochemical determination of the expression of endothelin receptors in bronchial smooth muscle and epithelium of healthy horses and horses affected by summer pasture-associated obstructive pulmonary disease.
Am J Vet Res, 67(2), 348-357.
https://doi.org/10.2460/ajvr.67.2.348 Publication
Researcher Affiliations
- Equine Health Studies Program, Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
MeSH Terms
- Animals
- Case-Control Studies
- Epithelium / metabolism
- Female
- Gene Expression Regulation
- Health
- Horse Diseases / metabolism
- Horses / metabolism
- Immunohistochemistry
- Lung / metabolism
- Lung / pathology
- Lung Diseases, Obstructive / metabolism
- Lung Diseases, Obstructive / veterinary
- Male
- Muscle, Smooth / metabolism
- Receptors, Endothelin / genetics
- Receptors, Endothelin / metabolism
- Seasons
Citations
This article has been cited 3 times.- Gauff FC, Patan-Zugaj B, Licka TF. Effect of short-term hyperinsulinemia on the localization and expression of endothelin receptors A and B in lamellar tissue of the forelimbs of horses. Am J Vet Res 2014 Apr;75(4):367-74.
- Venugopal CS, Holmes EP, Polikepahad S, Laborde S, Kearney M, Moore RM. Neurokinin receptors in recurrent airway obstruction: a comparative study of affected and unaffected horses. Can J Vet Res 2009 Jan;73(1):25-33.
- Polikepahad S, Haque M, Francis J, Moore RM, Venugopal CS. Characterization of endothelin receptors in the peripheral lung tissues of horses unaffected and affected with recurrent airway obstruction. Can J Vet Res 2008 Jul;72(4):340-9.
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