Immunohistochemical study of the distribution of adrenergic and peptidergic innervation in the equine uterus and the cervix.
Abstract: Little is known about neurogenic regulation of uterine contractility in mares. The present study investigated the distribution of adrenergic and peptidergic nerves in the mare uterus. Samples from the uterine horn, body and cervix were collected from 18 cyclic mares for immunohistochemistry. The uterus was well supplied with adrenergic nerves. A large number of tyrosine hydroxylase- and dopamine beta-hydroxylase-immunoreactive nerve bundles and fibres were present in the myometrium and endometrium in all regions of the uterus and cervix. These adrenergic nerve bundles and fibres travelled parallel to the muscle layers and were often associated with blood vessels. The density of peptidergic nerves was less than that of adrenergic nerves, but the pattern of distribution was similar. Neuropeptide Y-immunoreactive nerve fibres were the most abundant, whereas vasoactive intestinal polypeptide- and calcitonin gene-related peptide-immunoreactive nerve fibres were less frequently seen. Substance P-immunoreactive nerve fibres were the most sparse. Peptidergic nerves were distributed among the smooth muscle layers and near endometrial glands and were often associated with blood vessels in all regions of the uterus. The density of peptidergic nerve fibres was similar in the uterine horn and body but was slightly denser in the cervix. These findings indicate that uterine innervation may have an important role in controlling reproductive functions in mares.
Publication Date: 2001-07-27 PubMed ID: 11467978DOI: 10.1530/rep.0.1220275Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates the distribution of two types of nerves, adrenergic and peptidergic, in the uterus of mares. The findings reveal that both types of nerves are prevalent in the uterus with possible implications for reproductive function control in mares.
Detailed Analysis
- This research focuses on the neurogenic regulation of uterine contractility in mares which is not widely known. Such understanding is crucial as it could potentially give insights on how the reproductive functions in mares are controlled.
- The scholars conducted immunohistochemistry on samples from the uterine horn, body, and cervix obtained from 18 cyclic mares. They were specifically looking at the distribution of adrenergic and peptidergic nerves in these areas.
- Adrenergic nerves were found to be well supplied in the uterus. A significant number of these nerves, as detected by the presence of tyrosine hydroxylase and dopamine beta-hydroxylase, were found in the myometrium and endometrium in all regions of the uterus and cervix. They were often associated with blood vessels and travelled parallel to muscle layers.
- Peptidergic nerve density was less than that of adrenergic nerves. However, their distribution pattern was similar. Through the study, neuropeptide Y-immunoreactive nerve fibers were the most abundant. The study also observed less frequent instances of vasoactive intestinal polypeptide and calcitonin gene-related peptide-immunoreactive nerves. Substance P-immunoreactive nerve fibres were the scarcest of the group. These nerves were distributed among the smooth muscle layers and in close proximity to endometrial glands and were also found associated with blood vessels.
- The density of peptidergic nerve fibers was found to be similar in the uterine horn and body but slightly more dense in the cervix.
- In conclusion, the research findings suggest that uterine innervation, specifically the density and distribution of the adrenergic and peptidergic nerves, may play an important role in controlling reproductive functions in mares. This highlights the potential significance of these nerve types in the reproductive physiology of mares.
Cite This Article
APA
Bae SE, Corcoran BM, Watson ED.
(2001).
Immunohistochemical study of the distribution of adrenergic and peptidergic innervation in the equine uterus and the cervix.
Reproduction, 122(2), 275-282.
https://doi.org/10.1530/rep.0.1220275 Publication
Researcher Affiliations
- Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Center, Roslin, Midlothian, EH25 9RG, UK. dvmsbae@hotmail.com
MeSH Terms
- Animals
- Calcitonin Gene-Related Peptide / analysis
- Cervix Uteri / innervation
- Dopamine beta-Hydroxylase / analysis
- Female
- Horses
- Immunohistochemistry
- Nerve Fibers / enzymology
- Neuropeptide Y / analysis
- Neuropeptides / analysis
- Substance P / analysis
- Sympathetic Nervous System / anatomy & histology
- Sympathetic Nervous System / enzymology
- Tyrosine 3-Monooxygenase / analysis
- Uterus / innervation
- Vasoactive Intestinal Peptide / analysis
Citations
This article has been cited 10 times.- Jana B, Całka J. Effect of blocking of alpha1-adrenoreceptor isoforms on the noradrenaline-induced changes in contractility of inflamed pig uterus.. PLoS One 2023;18(2):e0280152.
- Wang X, Benagiano G, Liu X, Guo SW. Unveiling the Pathogenesis of Adenomyosis through Animal Models.. J Clin Med 2022 Mar 21;11(6).
- Yu J, Berga SL, Meng Q, Xia M, Kohout TA, van Duin M, Taylor RN. Cabergoline Stimulates Human Endometrial Stromal Cell Decidualization and Reverses Effects of Interleukin-1β In Vitro.. J Clin Endocrinol Metab 2021 Nov 19;106(12):3591-3604.
- Jana B, Całka J. Role of beta-adrenergic receptor subtypes in pig uterus contractility with inflammation.. Sci Rep 2021 Jun 1;11(1):11512.
- Aleksandrovych V, Kurnik-Łucka M, Bereza T, Białas M, Pasternak A, Cretoiu D, Walocha JA, Gil K. The Autonomic Innervation and Uterine Telocyte Interplay in Leiomyoma Formation.. Cell Transplant 2019 May;28(5):619-629.
- Mónica Brauer M, Smith PG. Estrogen and female reproductive tract innervation: cellular and molecular mechanisms of autonomic neuroplasticity.. Auton Neurosci 2015 Jan;187:1-17.
- Urra JA, Villaroel-Espíndola F, Covarrubias AA, Rodríguez-Gil JE, Ramírez-Reveco A, Concha II. Presence and function of dopamine transporter (DAT) in stallion sperm: dopamine modulates sperm motility and acrosomal integrity.. PLoS One 2014;9(11):e112834.
- Hu LX, Wang H, Rao M, Zhao XL, Yang J, Hu SF, He J, Xia W, Liu H, Zhen B, Di H, Xie C, Xia X, Zhu C. Alterations in the endometrium of rats, rabbits, and Macaca mulatta that received an implantation of copper/low-density polyethylene nanocomposite.. Int J Nanomedicine 2014;9:1127-38.
- Horn LC, Meinel A, Fischer U, Bilek K, Hentschel B. Perineural invasion in carcinoma of the cervix uteri--prognostic impact.. J Cancer Res Clin Oncol 2010 Oct;136(10):1557-62.
- Kimaro WH, Madekurozwa MC. Immunoreactivities to protein gene product 9.5, neurofilament protein and neuron specific enolase in the ovary of the sexually immature ostrich (Struthio camelus).. Exp Brain Res 2006 Aug;173(2):291-7.
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