Immunolocalization of succinate dehydrogenase in the esophagus epithelium of domesticated mammals.
Abstract: Using immunohistochemistry and transmission electron microscopy (TEM), the esophagus epithelia of seven domesticated mammals (horse, cattle, goat, pig, dog, laboratory rat, cat) of three nutrition groups (herbivorous, omnivorous, carnivorous) were studied to get first information about energy generation, as demonstrated by succinate dehydrogenase (SDH) activities. Distinct reaction intensities could be observed in all esophageal cell layers of the different species studied reflecting moderate to strong metabolic activities. The generally strong staining in the stratum basale indicated that new cells are continuously produced. The latter feature was confirmed by a thick, and in the horse generally highly active stratum spinosum. Only in the pig, reaction intensity variations occurred, obviously related to differences in physical feed quality or restricted feed allocation. The immunohistochemical results were corroborated by the presence of intact mitochondria in the esophageal cells of all species and nutrition types studied, except for the horse. Possible relationships between SDH reaction intensities and feed structure, mass or consistency are discussed.
Publication Date: 2013-05-10 PubMed ID: 23807297PubMed Central: PMC3794344DOI: 10.4081/ejh.2013.e18Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper studies the activities of succinate dehydrogenase (SDH), an enzyme involved in energy generation, in the esophagus epithelia of several domesticated mammals. Using immunohistochemical and electron microscopy techniques, the research showed differences in SDH activity that may be related to the mammal’s diet and physical feed quality.
Study Methods
- The researchers used immunohistochemistry and transmission electron microscopy (TEM) as their primary methods for studying the epithelial layers of the esophagus in seven types of domesticated animals: horses, cows, goats, pigs, dogs, rats, and cats.
- The animals selected for study belong to three nutrition groups: herbivores (feeding mostly on plants), omnivores (having a diet of both plants and meat), and carnivores (feeding predominantly on meat).
Findings
- The scientists were able to observe varying reaction intensities to the SDH activity across all the studied species, indicating a range of moderate to high metabolic activities. These activities could be related to the animals’ feed quality and diet type.
- A strong reaction was generally found in the stratum basale, the deepest layer of the esophagus epithelium, suggesting the continuous production of new cells. The thick and typically highly active stratum spinosum in horses further confirmed this observation.
- In pigs, however, variations in SDH activity were observed, possibly related to the quality of their feed or restricted feed allocation.
Mitochondrial Presence
- The research team’s results were substantiated by observing the presence of intact mitochondria in the esophageal cells of all studied animals, with the sole exception being horses. The absence of healthy mitochondria in horses’ esophagus cells might need further evaluation.
Implications and Conclusion
- The study discusses the potential relationships between SDH activity levels in the cells of the esophagus, and the structure, mass or consistency of the animals’ feed.
This could provide valuable insights for dietary planning and nutritional management in domestic animals, particularly those bred for agriculture or livestock purposes.
Cite This Article
APA
Meyer W, Kacza J, Hornickel IN, Schoennagel B.
(2013).
Immunolocalization of succinate dehydrogenase in the esophagus epithelium of domesticated mammals.
Eur J Histochem, 57(2), e18.
https://doi.org/10.4081/ejh.2013.e18 Publication
Researcher Affiliations
- Institute for Anatomy, University of Veterinary Medicine Hannover Foundation, 30173 Hannover, Germany. wilfried.meyer@tiho-hannover.de
MeSH Terms
- Animals
- Cats
- Cattle
- Diet / veterinary
- Dogs
- Epithelium / enzymology
- Esophagus / enzymology
- Feeding Behavior / physiology
- Female
- Goats
- Horses
- Immunohistochemistry
- Male
- Microscopy, Electron, Transmission
- Rats
- Succinate Dehydrogenase / metabolism
- Swine
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Citations
This article has been cited 3 times.- Martyniuk K, Ziółkowska N, Hanuszewska-Dominiak M, Szyryńska N, Lewczuk B. Histology and Ultrastructure of the Esophagus in European Beaver (Castor fiber) Displays Features Adapted to Seasonal Changes in Diet. Animals (Basel) 2023 Feb 11;13(4).
- Malatesta M. Ultrastructural histochemistry in biomedical research: Alive and kicking. Eur J Histochem 2018 Nov 7;62(4).
- Pellicciari C. Histochemistry in biology and medicine: a message from the citing journals. Eur J Histochem 2015 Dec 23;59(4):2610.
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