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Food research international (Ottawa, Ont.)2022; 156; 111305; doi: 10.1016/j.foodres.2022.111305

Impact of different dietary regimens on the lipidomic profile of mare’s milk.

Abstract: Despite mare's milk being highly nutritious and beneficial to human health, the effects of different dietary regimens on the lipid profile of mare's milk remain to be completely understood. Herein we employed targeted multiple reaction monitoring-based lipidomics with ultra-high performance liquid chromatography-quadrupole time-of-flight-mass spectrometry to compare the milk lipid profiles of mares fed: pasture grass (P), corn stover and concentrate (H), or cornsilage and concentrate (S). Overall, 461 lipids belonging to 17 subclasses were identified; 261 significantly different lipids were identified on applying the following criteria: false discovery rate-adjusted P < 0.05, variable importance in projection ≥ 1.0, and fold change ≥ 2.0 or ≤ 0.5. The number of significantly different lipids decreased in the order of P > S > H. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis using differential lipids led to the identification of 34, 32, and 31 metabolic pathways on pairwise comparisons between the P and H, P and S, and S and H groups, respectively. The milk of mares in the P group showed significantly higher levels of specific lipids, including triacylglycerols, phosphatidylcholines, and phosphatidylserines, which are known to participate in polyunsaturated fatty acid and lipid digestion and absorption-related metabolic pathways. Altogether, our data provide comprehensive insights into the correlations between feeding systems and quality of mare's milk in terms of bioactive profile.
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Publication Date: 2022-04-29 PubMed ID: 35651065DOI: 10.1016/j.foodres.2022.111305Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigated the effects of different diets on the lipid content in mare’s milk. Using complex mass spectroscopy techniques, the study found dietary regimens notably influence the milk’s lipidomic makeup, highlighting the link between feeding systems and the milk’s bioactive profile.

Research Methods

  • The study employed a targeted multiple reaction monitoring-based lipidomics technique. This involved advanced laboratory techniques including ultra-high performance liquid chromatography-quadrupole time-of-flight-mass spectrometry, enabling the identification and quantification of lipids in the milk of mares fed different diets.
  • The diets analysed were pasture grass (P), corn stover with concentrate (H), or cornsilage with concentrate (S).
  • The criteria for significantly different lipids to be recognised in the investigation were a false discovery rate-adjusted P-value of less than 0.05, a variable importance in projection of greater than or equal to 1.0, and a fold change of greater than or equal to 2.0 or less than or equal to 0.5.
  • Through analysis of differential lipids, a number of metabolic pathways were identified. These were found through using the Kyoto Encyclopedia of Genes and Genomes.

Key Findings

  • A total of 461 lipids belonging to 17 subclasses were identified. Of these, 261 lipids were significantly different based on the set criteria.
  • The number of significantly different lipids reduced in the order of P > S > H, denoting that the diet influenced the lipid profile of mare’s milk.
  • Milk from mares in the P group exhibited significantly higher levels of specific lipids including triacylglycerols, phosphatidylcholines, and phosphatidylserines. These lipids are known to participate in polyunsaturated fatty acid and lipid digestion and absorption-related metabolic pathways.
  • The research therefore shows the impact of diet on the lipidome of mare’s milk, specifically demonstrating how certain feeding systems can enhance the quality of the milk in terms of its bioactive profile.

Cite This Article

APA
Deng L, Yang Y, Li Z, Li J, Zhu Y, Meng Q, Liu J, Wang X. (2022). Impact of different dietary regimens on the lipidomic profile of mare’s milk. Food Res Int, 156, 111305. https://doi.org/10.1016/j.foodres.2022.111305

Publication

Food research international (Ottawa, Ont.)
ISSN: 1873-7145
NlmUniqueID: 9210143
Country: Canada
Language: English
Volume: 156
Pages: 111305

Researcher Affiliations

Deng, Liang
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China. Electronic address: ldeng@syau.edu.cn.
Yang, Yixin
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
Li, Zheng
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
Li, Jing
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
Zhu, Yiping
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
Meng, Qingze
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
Liu, Jingze
  • Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, PR China.
Wang, Xuefan
  • Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.

MeSH Terms

  • Animals
  • Diet / veterinary
  • Fatty Acids, Unsaturated / analysis
  • Female
  • Horses
  • Humans
  • Lipidomics
  • Mass Spectrometry
  • Milk / chemistry

Citations

This article has been cited 10 times.
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    doi: 10.1016/j.fochx.2023.101024pubmed: 38144754google scholar: lookup
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