Impact of low-dose ozone supplementation on motility parameters and bacterial growth in horse cryopreserved semen.
Abstract: Two studies were conducted to evaluate the use of medical ozone (O) in commercial extenders for equine semen cryopreservation. In the first study (Study 1), 0, 5, and 15 µg/mL of O were added to diluents of refrigerated or frozen semen. Samples were evaluated for sperm kinematics at different time points for the chilled samples and after a thermoresistence test for the frozen/thawed samples. In the second study (Study 2), 0, 5, and 10 µg/mL of O were added to an antibiotic-free diluent for refrigerated semen for comparison with the control group in which semen was diluted in the same diluent enriched with antibiotics. Semen sample kinematics were analyzed and an aliquot was collected after ozonification for bacteriological analyses. For Study 1 no difference was found comparing all the kinematic parameters analyzed over time, in the various treatments ( > 0.05). In Study 2 the absence of antibiotics did not affect the kinematic parameters compared to the control ( > 0.05). However when antibiotics were added, a smaller number of bacterial colony-forming units were detected compared to samples without antibiotics and without or with different O supplementations. In conclusion, O treatment at low dosages did not affect the semen kinematics, although it was ineffective in preventing bacterial overgrowth. Higher O concentrations should be evaluated to explore the possibility of reducing the use of antibiotics in equine sperm conservation.
© 2024 The Authors. Published by Elsevier Ltd.
Publication Date: 2024-07-21 PubMed ID: 39135617PubMed Central: PMC11318542DOI: 10.1016/j.vas.2024.100380Google Scholar: Lookup
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Summary
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The research investigates the use of medical ozone in diluents for equine semen cryopreservation to review its impact on motility parameters and bacterial growth. The study concludes that while the ozone treatment doesn’t affect semen kinematics, it fails to prevent bacterial overgrowth, suggesting the need for further evaluation of higher ozone concentrations.
Objective of the Research
- The main goal of this research was to assess the potential effects of adding low-dose ozone to extenders commonly used in equine semen cryopreservation on sperm kinetics and bacterial growth.
Methodology of the Two Studies
- Two experiments were conducted, involving the addition of different ozone doses to semen diluents and comparing semen kinetics and bacterial overgrowth results.
- The first study (Study 1) added O levels of 0, 5, and 15 µg/mL to refrigerated or frozen semen diluents. Sperm kinematics were then evaluated over time for chilled samples and after a thermoresistence test for frozen/thawed samples.
- The second study (Study 2) introduced 0, 5, and 10 µg/mL of O to antibiotic-free diluent for refrigerated semen while a control group got the same diluent with antibiotics. Semen kinetics were analyzed, and an aliquot was collected after ozonification for bacterial analyses.
Results and Findings
- In Study 1, no significant difference was observed between all the kinematic parameters across time and various treatments (p > 0.05), suggesting the addition of O made no impact on these parameters.
- In Study 2, the absence of antibiotics did not affect the kinematic parameters compared to the control (p > 0.05).
- When antibiotics were included, a smaller number of bacterial colony-forming units were detected compared to samples without antibiotics and with or without different O supplementations.
Conclusion
- The utilization of O treatment at the stated doses had no impact on semen kinetics, but it failed to control the bacterial growth within the samples.
- Based on these findings, there’s a call for further evaluation of higher ozone concentrations. This could be crucial to reduce the reliance on antibiotics in equine semen preservation.
Cite This Article
APA
Fanelli D, Moroni R, Sala G, Melanie P, Tarabella I, Telleschi N, Maltinti S, Giorgi M, Barsotti G, Passamonti F, Marmorini P, Rota A, Camillo F, Panzani D.
(2024).
Impact of low-dose ozone supplementation on motility parameters and bacterial growth in horse cryopreserved semen.
Vet Anim Sci, 25, 100380.
https://doi.org/10.1016/j.vas.2024.100380 Publication
Researcher Affiliations
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126, Perugia, Italy.
- Private Practitioner, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
- Veterinary Sciences Department, Pisa University, Via Livornese 56124, San Piero a Grado, Pisa, Italy.
Conflict of Interest Statement
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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