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Antioxidants (Basel, Switzerland)2022; 11(2); 417; doi: 10.3390/antiox11020417

Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance.

Abstract: This study investigated whether the activities of the antioxidant components of donkey seminal plasma (SP)-both enzymatic (superoxide dismutase (SOD), catalase-like (CAT), glutathione peroxidase-like (GPX), and paraoxonase type 1 (PON1)) and non-enzymatic (measured in terms of total thiol, copper-reducing antioxidant capacity (CUPRAC), ferric-reducing ability of plasma (FRAP), and Trolox equivalent antioxidant capacity (TEAC))-and oxidative stress index (OSI) are related to sperm cryotolerance. For this purpose, 15 ejaculates from jackasses (one per individual) were collected and split into two aliquots. The first one was used for measuring the activities levels of enzymatic and non-enzymatic antioxidants and OSI in SP, whereas the other aliquot was cryopreserved. Before cryopreservation, sperm quality parameters (concentration, motility, and viability) were evaluated. After thawing, sperm motility, plasma membrane integrity, lipid disorder, mitochondrial membrane potential, reactive oxygen species (ROS), and calcium intracellular levels were also determined. Based on the percentages of total motility (TM) and of sperm with an intact plasma membrane (SYBR14/PI) after thawing, samples were classified as good-freezability (GFE) or poor-freezability (PFE) ejaculates through cluster analyses. The SP activity levels of enzymatic (SOD and PON1) and non-enzymatic antioxidants (CUPRAC, FRAP, and TEAC) were higher ( < 0.05) in GFE than in PFE, whereas SP-OSI was higher ( < 0.05) in PFE than in GFE. In addition, the activity levels of SOD, PON1, GPX, CUPRAC, FRAP, and TEAC were positively ( < 0.05) related to post-thaw sperm motility and plasma membrane integrity and negatively to intracellular ROS levels. The SP-OSI was negatively correlated ( < 0.05) to post-thaw sperm quality parameters and positively to intracellular ROS levels. It can thus be concluded that donkey SP antioxidants are related to sperm cryotolerance and that measurements of antioxidants PON1, SOD, CUPRAC, FRAP, and TEAC, as well as SP-OSI, could be used as markers of sperm cryotolerance. Further research addressing the relationship of these antioxidants and SP-OSI with sperm cryotolerance and their potential use as freezing markers is warranted.
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Publication Date: 2022-02-18 PubMed ID: 35204299PubMed Central: PMC8869541DOI: 10.3390/antiox11020417Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article examined the link between antioxidant activity in donkey sperm and its ability to endure freezing and thawing processes. The article highlights the potential value of antioxidants as indicators of sperm freeze-tolerance, suggesting that measurements of specific antioxidants, alongside an oxidative stress index, could help predict the viability of preserved sperm samples.

Overview of the Research & Methodology

  • The study set out to understand the relationship between the antioxidant activity in donkey seminal plasma (SP) and sperm’s ability to tolerate freezing and thawing, a process known as cryotolerance.
  • Both enzymatic antioxidants (super oxide dismutase, catalase-like, glutathione peroxidase-like, and paraoxonase type 1) and non-enzymatic antioxidants (total thiol, copper-reducing antioxidant capacity, ferric-reducing ability of plasma and Trolox equivalent antioxidant capacity) were assessed.
  • The team used 15 ejaculates from separate donkeys; each sample was split into two portions. One portion was used to measure antioxidant activity and the oxidative stress index in SP, while the other was frozen and thawed.
  • Quality parameters like sperm concentration, motility, and viability were evaluated before freezing. Post-thaw, measures such as sperm motility, plasma membrane integrity, lipid disorder, mitochondrial membrane potential, reactive oxygen species, and calcium intracellular levels were determined.

Results and Findings

  • The ejaculates were classified as good-freezability (GFE) or poor-freezability (PFE) based on post-thaw sperm motility and plasma membrane integrity.
  • In GFE samples, levels of both enzymatic and non-enzymatic antioxidants were higher compared to PFE samples, while the oxidative stress index was lower.
  • There was a positive correlation between the levels of enzymatic and non-enzymatic antioxidants and the post-thaw quality of the sperm. Inversely, there was a negative relationship between these antioxidant levels and intracellular reactive oxygen species levels.
  • The stress index showed a negative relationship with post-thaw sperm motility and a positive relationship with increased reactive oxygen species.

Conclusions

  • The researchers conclude that there’s a notable relationship between donkey SP antioxidants and sperm cryotolerance. Antioxidants such as SOD, PON1, CUPRAC, FRAP, and TEAC, along with the oxidative stress index, could serve as potential markers for sperm freeze-tolerance.
  • The research suggests further investigation into these antioxidant markers and their effectiveness in successful sperm freezing and thawing. This could be a significant contribution to fertility treatments and animal breeding programs.

Cite This Article

APA
Catalán J, Yánez-Ortiz I, Tvarijonaviciute A, González-Arostegui LG, Rubio CP, Yeste M, Miró J, Barranco I. (2022). Impact of Seminal Plasma Antioxidants on Donkey Sperm Cryotolerance. Antioxidants (Basel), 11(2), 417. https://doi.org/10.3390/antiox11020417

Publication

Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
NlmUniqueID: 101668981
Country: Switzerland
Language: English
Volume: 11
Issue: 2
PII: 417

Researcher Affiliations

Catalán, Jaime
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
  • Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain.
  • Faculty of Veterinary Medicine, University of Teramo, Loc. Piano d'Accio, IT-64100 Teramo, Italy.
Yánez-Ortiz, Iván
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
  • Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain.
Tvarijonaviciute, Asta
  • Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, ES-30100 Murcia, Spain.
  • Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, ES-30100 Murcia, Spain.
González-Arostegui, Luis Guillermo
  • Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, ES-30100 Murcia, Spain.
  • Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, ES-30100 Murcia, Spain.
Rubio, Camila P
  • Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, ES-30100 Murcia, Spain.
  • Department of Animal and Food Science, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
Yeste, Marc
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, Spain.
  • Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain.
Miró, Jordi
  • Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, ES-08193 Cerdanyola del Vallès, Spain.
Barranco, Isabel
  • Department of Veterinary Medical Sciences, University of Bologna, IT-40064 Ozzano dell'Emilia, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

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