Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols.
Abstract: One of the challenges for those attempting to cryopreserve stallion spermatozoa is dealing with the stallion to stallion variability in the cryosurvival of their semen. In the dairy industry, each bull stud, essentially utilizes a single cryopreservation technique, and bulls that produce sperm that do not cryopreserve well using that technique are replaced by other bulls. However, replacing stallions is unlikely to prove acceptable to the equine industry, where specific genotypes are desired. Instead, to increase the number of stallions that can be effectively utilized for cryopreserved semen production, it is likely that more than one method for cryopreserving sperm will be necessary. This manuscript reviews some of the processes involved in cryopreservation, how individual sperm physiology affects the ability to survive freezing and thawing, and how cryopreservation protocols can be customized to maximize sperm cryosurvival on an individual stallion basis.
Publication Date: 2007-11-26 PubMed ID: 18178040DOI: 10.1016/j.anireprosci.2007.11.010Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
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Summary
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This research examines the differences in survival rates of frozen stallion sperm, highlighting that different cryopreservation methods might be necessary to optimize results for individual stallions.
Cryopreservation of Stallion Semen
- The process of freezing and storing sperm, known as cryopreservation, presents challenges specific to stallions.
- Unlike the dairy industry, where bulls whose sperm does not cryopreserve well are replaced, such replacement is less acceptable in the equine industry where specific genetic types are preferred.
Stallion-to-Stallion Variability in Cryosurvival
- The study recognizes significant variation in semen cryosurvival rates among different stallions, suggesting that a single cryopreservation method might not be effective universally.
- Therefore, the study argues for customizing cryopreservation protocols depending on the specific physiological characteristics of individual stallions to maximize sperm survival rates.
Processes Involved in Cryopreservation
- The paper also provides a review of some of the critical processes involved in cryopreservation, considering how specific physiological factors of individual sperm cells affect their survival rates when frozen and thawed.
Need for Multiple Cryopreservation Techniques in the Equine Industry
- Due to the stallion-specific requirements for successful cryopreservation, the research concludes that different methods of cryopreservation may be necessary in the equine industry.
- This approach aims at increasing the number of stallions utilized effectively in producing cryopreserved semen to maintain the desired genotypes.
Cite This Article
APA
Loomis PR, Graham JK.
(2007).
Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols.
Anim Reprod Sci, 105(1-2), 119-128.
https://doi.org/10.1016/j.anireprosci.2007.11.010 Publication
Researcher Affiliations
- Select Breeders Service, Inc., 1088 Nesbitt Road, Colora, MD 21917, USA. paulloomis@selectbreeders.com
MeSH Terms
- Animals
- Cryopreservation / methods
- Cryopreservation / veterinary
- Horses / physiology
- Male
- Semen Preservation / methods
- Semen Preservation / veterinary
- Spermatozoa / physiology
Citations
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