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Home / Equine Research Bank / Anim Reprod Sci / Implementing an open-access CASA software for the assessment...
Animal reproduction science2016; 176; 11-19; doi: 10.1016/j.anireprosci.2016.11.003

Implementing an open-access CASA software for the assessment of stallion sperm motility: Relationship with other sperm quality parameters.

Abstract: Setting an open-access computer assisted sperm analysis (CASA) may benefit the evaluation of motility in mammalian sperm, especially when economic constraints do not allow the use of a commercial system. There have been successful attempts to develop such a device in Zebra fish sperm and the system has been used in very few studies on mammalian spermatozoa. Against this background, the present study aimed at developing an open-access CASA system for mammalian sperm using the horse as a model and based upon the Image J software previously established for Zebra fish sperm. Along with determining the sperm progressive motility and other kinetic parameters (such as amplitude of lateral head displacement), the "results" window was adjusted to simplify subsequent statistical analyses. The path window was enriched with colored sperm trajectories on the basis of the subpopulation they belong to and a number that allowed the sperm track to be associated to the sperm motility data shown in the "results" window. Data obtained from the novel plugin (named as CASA_bgm) were compared with those of the commercial CASA Hamilton-Thorn IVOS Vers.12, through Bland Altman's plots. While the percentage of total and progressive motile sperm, VCL, VAP, VSL, LIN and STR and ALH were in agreement with those obtained with the commercial system, BCF significantly differed between the two systems probably due to their settings. Interestingly, a positive and significant correlation between the percentages of total motile sperm evaluated through CASA_bgm and those showing high mitochondrial membrane potential evaluated by JC-1 staining was found. In conclusion, CASA_bgm ImageJ plugin could be useful and reliable for stallion sperm motility analysis and it is our aim to apply this system to other mammalian species.
Copyright © 2016 Elsevier B.V. All rights reserved.
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Publication Date: 2016-11-13 PubMed ID: 27887759DOI: 10.1016/j.anireprosci.2016.11.003Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research aimed to develop an open-access computer assisted sperm analysis (CASA) system for mammalian sperm, using horses as a model, which compares favorably with commercial systems and shows a positive correlation with high mitochondrial membrane potential.

Introduction

  • The main goal of this research was to create an open-source computer assisted sperm analysis (CASA) for mammalian sperm, specifically for horses, given successful prior experimentation with Zebra fish sperm.
  • The CASA system is intended to be a valuable tool for evaluating sperm motility, especially in contexts where financial restraints make commercial systems unfeasible.

Methodology

  • The open-access CASA system was developed on the basis of the Image J software, previously used for Zebra fish sperm.
  • A variety of sperm kinetic parameters were monitored, including progressive motility and amplitude of lateral head displacement (ALH).
  • Data generated by the newly developed plugin, referred to as CASA_bgm, was compared with data sourced from a commercial system—Hamilton-Thorn IVOS Vers.12.

Results

  • There was a general agreement between the commercial system and the newly developed open-access CASA system regarding the percentage of total and progressive motile sperm, VSL, VCL, VAP, LIN, STR, and ALH.
  • However, there was a notable discrepancy in BCF data between the two systems, possibly due to differences in their settings.
  • A significant and positive correlation was found between the percentages of total motile sperm evaluated through CASA_bgm and those showing a high mitochondrial membrane potential, assessed via JC-1 staining.

Conclusion

  • This research concludes that the CASA_bgm ImageJ plugin might be a useful and dependable alternative for stallion sperm motility analysis.
  • The team expresses desire to extend application of this system to other mammalian species in future.

Cite This Article

APA
Giaretta E, Munerato M, Yeste M, Galeati G, Spinaci M, Tamanini C, Mari G, Bucci D. (2016). Implementing an open-access CASA software for the assessment of stallion sperm motility: Relationship with other sperm quality parameters. Anim Reprod Sci, 176, 11-19. https://doi.org/10.1016/j.anireprosci.2016.11.003

Publication

Animal reproduction science
ISSN: 1873-2232
NlmUniqueID: 7807205
Country: Netherlands
Language: English
Volume: 176
Pages: 11-19
PII: S0378-4320(16)30314-1

Researcher Affiliations

Giaretta, Elisa
  • DIMEVET, Department of Veterinary Medical Sciences, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy.
Munerato, Mauro
  • Private researcher.
Yeste, Marc
  • Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17071 Girona, Catalonia, Spain.
Galeati, Giovanna
  • DIMEVET, Department of Veterinary Medical Sciences, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy.
Spinaci, Marcella
  • DIMEVET, Department of Veterinary Medical Sciences, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy.
Tamanini, Carlo
  • DIMEVET, Department of Veterinary Medical Sciences, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy.
Mari, Gaetano
  • DIMEVET, Department of Veterinary Medical Sciences, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy; AUB INFA National Institute of Artificial Insemination, Via Gandolfi 16, 40057 Cadriano, BO, Italy.
Bucci, Diego
  • DIMEVET, Department of Veterinary Medical Sciences, Via Tolara di Sopra, 50, 40064 Ozzano dell'Emilia, BO, Italy. Electronic address: diego.bucci3@unibo.it.

MeSH Terms

  • Animals
  • Horses / physiology
  • Image Processing, Computer-Assisted
  • Male
  • Semen Analysis / methods
  • Semen Analysis / veterinary
  • Software
  • Sperm Motility / physiology
  • Spermatozoa / physiology

Citations

This article has been cited 12 times.
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