Implications of urine F2-isoprostane metabolite concentration in horses with colic and its potential use as a predictor for surgical intervention.
Abstract: F2-isoprostanes have been used extensively to quantify lipid peroxidation in association with risk factors in various diseases. Horses with colic may have intestinal ischaemia and/or inflammation characterised by oxidative stress and increased production of isoprostanes. Objective: To gather preliminary data regarding the feasibility of using urine F2-isoprostanes and isoprostane metabolites as early screening tools for the presence of gastrointestinal disease requiring surgical intervention in horses and ultimately develop a stall-side test capable of identifying these horses as early as possible for timely referral. Methods: Concentrations of urine isoprostane and isoprostane metabolite were determined by mass spectroscopy and normalised to urine creatinine (Cr) concentrations in urine samples from 42 healthy control horses and 43 horses with gastrointestinal pain or colic. Results: Horses with colic were treated medically (n = 21) or surgically (n = 22). Mean ± s.d. concentrations of urine isoprostane and isoprostane metabolite were significantly higher in horses with colic (2.94 ± 1.69 and 0.31 ± 0.22 ng/mg Cr, respectively), compared to control horses (1.89 ± 1.39 and 0.22 ± 0.08 ng/mg Cr, respectively). Mean urine isoprostane metabolite concentrations were significantly higher in horses undergoing surgery (0.38 ± 0.28 ng/mg Cr) compared to controls and medical colics (0.26 ± 0.11 ng/mg Cr). Nonsurvivors had significantly higher mean urine isoprostane metabolite concentrations (0.47 ± 0.39 ng/mg Cr) than control or surviving colic horses (0.29 ± 0.24 ng/mg Cr). Conclusions: Measurement of urine isoprostane metabolite concentration may be a useful prognostic indicator in equine colic. Conclusions: Urine isoprostane metabolites may aid in early recognition of surgical colic. Isoprostanes are a potential therapeutic target to prevent further systemic and gastrointestinal tissue injury in horses with colic.
© 2011 EVJ Ltd.
Publication Date: 2011-08-04 PubMed ID: 21790752DOI: 10.1111/j.2042-3306.2011.00384.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the potential of urine F2-isoprostanes as a predictive marker for surgical intervention in horses suffering from colic. This is based on the premise that colic in horses typically generates oxidative stress leading to higher concentrations of isoprostanes in the urine.
Overview of the Study
- The primary objective of the study was to collate preliminary data on the potential of using urine F2-isoprostanes and their metabolites as early markers for identifying horses with gastrointestinal diseases that may necessitate a surgical intervention.
- This was conducted with the motivation to eventually develop a readily available “stall-side” test that faster identifies colic in horses for timely referrals.
Study Methodology
- The researchers measured the concentration of urine isoprostane and its metabolites using a method known as mass spectroscopy.
- The concentrations were normalized based on urine creatinine levels in 42 healthy horses and 43 horses diagnosed with colic or gastrointestinal pain.
Study Results
- Of the 43 horses diagnosed with colic, 21 were treated medically while 22 needed surgical treatment.
- Higher mean concentrations of urine isoprostane and its metabolites were observed in horses with colic, as compared to the control horses.
- Especially, horses requiring surgical intervention showed significantly higher mean urine isoprostane metabolite concentrations compared to controls and medically-treated colic horses.
- Horses that did not survive exhibited the highest mean urine isoprostane metabolite concentrations compared to controls or surviving colic horses.
Conclusion
- The study concludes that the concentration of urine isoprostane metabolite could serve as a useful indicator in predicting the prognosis in cases of equine colic.
- The metabolites may also help in early recognition of colic cases that would need surgery.
- The research also suggests a potentially new therapeutic target, namely isoprostanes, which could help prevent further damage to the systemic and gastrointestinal tissues in horses affected by colic.
Cite This Article
APA
Noschka E, Werre SR, Crisman MV, Thatcher CD, Milne GL, Dahlgren LA.
(2011).
Implications of urine F2-isoprostane metabolite concentration in horses with colic and its potential use as a predictor for surgical intervention.
Equine Vet J Suppl(39), 34-41.
https://doi.org/10.1111/j.2042-3306.2011.00384.x Publication
Researcher Affiliations
- School of Animal and Veterinary Sciences, The University of Adelaide, Australia.
MeSH Terms
- Animals
- Colic / urine
- Colic / veterinary
- Digestive System Surgical Procedures / veterinary
- F2-Isoprostanes / metabolism
- F2-Isoprostanes / urine
- Female
- Horse Diseases / urine
- Horses
- Male
- Predictive Value of Tests
Citations
This article has been cited 1 times.- Putman AK, Contreras GA, Sordillo LM. Isoprostanes in Veterinary Medicine: Beyond a Biomarker. Antioxidants (Basel) 2021 Jan 20;10(2).
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