In vitro and in vivo evaluation of ferric-hyaluronate implants for delivery of amikacin sulfate to the tarsocrural joint of horses.
Abstract: To assess the antimicrobial elution characteristics, toxicity, and antimicrobial activity of amikacin-impregnated ferric-hyaluronate implants (AI-FeHAI) for amikacin delivery to the tarsocrural joint of horses. Methods: Experimental study. Methods: AI-FeHAI implants, equine cartilage, and synovium, and horses (n=6). Methods: In vitro study: Five AI-FeHAI were placed in saline solution with daily replacement until implant degradation. Eluent was tested for amikacin concentration and bioactivity. Synovial and cartilage explants were incubated in the presence or absence of AI-FeHAI for 72 hours and subsequently assessed for morphology, viability, and composition. Synovial explants were incubated with Staphylococcus aureus in the presence or absence of AI-FeHAI. Spent medium was cultured daily and explants were assessed for morphology and viability after 96 hours. In vivo study: AI-FeHAI were placed in 6 tarsocrural joints. Standard cytologic analysis and amikacin concentration (SFAC) were determined in synovia obtained regularly for 28 days thereafter. Similar analyses were conducted after a single intra-articular injection of amikacin 6 months later. Results: In vitro study: Amikacin concentrations exceeded 16 microg/mL and inhibited S. aureus growth for 8 days. AI-FeHAI had no effect on cartilage explants. AI-FeHAI eliminated bacteria from synovial explants. In vitro study: After AI-FeHAI placement, SFAC was highest (140.78+63.81 microg/mL) at first sampling time. By 24 hours SFAC was <16 microg/mL. After intra-articular injection, SFAC was the highest (377.91 +/- 40.15 microg/mL) at first sampling time. By 48 hours SFAC was <16 microg/mL. Conclusions: A single intra-articular amikacin injection demonstrated superior pharmacokinetics than AI-FeHAI prepared as described. Conclusions: AI-FeHAI cannot be recommended for clinical use.
Publication Date: 2009-06-23 PubMed ID: 19538672DOI: 10.1111/j.1532-950X.2009.00518.xGoogle Scholar: Lookup
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- Controlled Clinical Trial
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research paper examines the effectiveness of amikacin-impregnated ferric-hyaluronate implants for delivering antibiotics to the tarsocrural joint of horses and concludes that a single intra-articular injection of amikacin yields better results.
Research Design and Methodology
- The research conducted both in vitro and in vivo experiments to understand the elution characteristics, toxicology, and antimicrobial activity of the amikacin-impregnated ferric-hyaluronate implants, also referred to as AI-FeHAI.
- In the in vitro study, five AI-FeHAIs were placed in saline solution, with the solution replaced daily until the implant degraded. The eluent was tested for amikacin concentration and bioactivity. Additionally, equine synovium and cartilage explants were exposed to AI-FeHAI and assessed.
- In the in vivo experiment, the AI-FeHAI were tested on six horses over a 28-day period, with synovial fluid samples collected regularly. Amikacin concentration was measured after each collection.
Findings from the In Vitro Study
- Amikacin concentrations went beyond 16 micrograms per milliliter and inhibited the growth of the bacteria Staphylococcus aureus for approximately eight days.
- AI-FeHAI did not have any impact on cartilage explants.
- AI-FeHAI was able to eliminate bacteria from synovial explants.
Findings from the In Vivo Study
- The maximum concentration of amikacin achieved immediately after AI-FeHAI placement was 140.78+63.81 micrograms per milliliter, but it fell below 16 micrograms per milliliter within 24 hours.
- Following a single intra-articular injection, the maximum concentration of amikacin peaked at 377.91 +/- 40.15 micrograms per milliliter, exhausting below 16 micrograms per milliliter only after 48 hours.
Conclusions and Recommendations
- The pharmacokinetics of the amikacin was significantly better after a single intra-articular injection compared to the delivery via AI-FeHAI prepared in this study.
- Based on the study’s findings, the use of AI-FeHAI for clinical use cannot be recommended.
Cite This Article
APA
Cribb NC, Bouré LP, Hanna WJ, Akens MK, Mattson SE, Monteith GJ, Weese JS.
(2009).
In vitro and in vivo evaluation of ferric-hyaluronate implants for delivery of amikacin sulfate to the tarsocrural joint of horses.
Vet Surg, 38(4), 498-505.
https://doi.org/10.1111/j.1532-950X.2009.00518.x Publication
Researcher Affiliations
- Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada. ncribb@uoguelph.ca
MeSH Terms
- Absorbable Implants / veterinary
- Amikacin / administration & dosage
- Amikacin / pharmacokinetics
- Animals
- Drug Delivery Systems
- Horses
- Hyaluronic Acid / chemistry
- Injections, Intra-Articular / veterinary
- Iron / chemistry
- Tarsus, Animal
Citations
This article has been cited 1 times.- Guardabassi L, Apley M, Olsen JE, Toutain PL, Weese S. Optimization of Antimicrobial Treatment to Minimize Resistance Selection. Microbiol Spectr 2018 May;6(3).
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