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Home / Equine Research Bank / Vet Ophthalmol / In vitro antibacterial efficacy of autologous conditioned pl...
Veterinary ophthalmology2023; 26(6); 548-554; doi: 10.1111/vop.13120

In vitro antibacterial efficacy of autologous conditioned plasma and amniotic membrane eye drops.

Abstract: To determine the in vitro antibacterial efficacy of equine and canine autologous conditioned plasma (ACP) and amniotic membrane extract eye drops (AMEED) against aerobic bacteria common to the corneal surface. Methods: Canine (n = 4) and equine (n = 4) anticoagulated whole blood samples were sterilely collected, pooled for each species, and processed using the Arthrex ACP® Double-Syringe System. Platelet counts were performed on ACP and pooled blood. AMEED were obtained from a commercial source. An electronic medical records search (2013-2022) identified aerobic bacteria cultured from canine and equine corneal ulcers at Mississippi State University College of Veterinary Medicine (MSU-CVM). Ten commonly isolated bacteria for each species were collected from cultures submitted to the MSU-CVM Microbiology Diagnostic Service and frozen at -80°C. The Kirby-Bauer disk diffusion method was used to determine the sensitivities of these isolates to ACP and AMEED. Bacterial isolates were plated onto Mueller-Hinton +5% sheep blood agar and blank sterile discs saturated with 20 μL of ACP or AMEED were tested in duplicate. Imipenem discs served as positive controls and blank discs as negative controls. Zones of inhibition were measured at 18 h. Results: ACP platelet counts were 1.06 and 1.65 times higher than blood for equine and canine samples, respectively. Growth of a multi-drug resistant Enterococcus faecalis was partially inhibited by canine and equine ACP. AMEED did not inhibit growth of any examined bacteria. Conclusions: Canine and equine ACP partially inhibited E. faecalis growth in vitro. Further studies using varying concentrations of ACP against bacterial isolates from corneal ulcers are warranted.
© 2023 American College of Veterinary Ophthalmologists.
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Publication Date: 2023-06-14 PubMed ID: 37317517DOI: 10.1111/vop.13120Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the antibacterial properties of autologous conditioned plasma (ACP) and amniotic membrane extract eye drops (AMEED) against aerobic bacteria often found on the corneal surface of dogs and horses. The results revealed that growth of a certain type of bacteria was partially inhibited by both canine and equine ACP, while AMEED showed no efficacy.

Methods

  • The research involved samples of anticoagulated whole blood taken from four dogs and four horses. These were sterilized, pooled together respectively for each species, and processed using a particular double-syringe system.
  • The team performed platelet counts on both the ACP and the pooled blood, and also obtained AMEED from a commercial source.
  • By referring to electronic health records from 2013 to 2022, they identified the different types of aerobic bacteria found in corneal ulcers in dogs and horses treated at Mississippi State University College of Veterinary Medicine.
  • From this, they collected the ten most commonly isolated bacteria for each species from submitted cultures and stored these at extremely low temperatures.
  • To determine the bacteria’s sensitivity to both ACP and AMEED, the researchers used the Kirby-Bauer Disk Diffusion Method.
  • These bacterial cultures were plated onto agar, and blank sterile discs saturated with either ACP or AMEED were tested in duplicate. A particular antibiotic served as a positive control, while blank discs served as the negative control.
  • After 18 hours, the researchers measured the zones of inhibition or areas where bacterial growth was prevented.

Results

  • Measurements revealed that the counts of platelets in ACP samples were 1.06 times higher in horses and 1.65 times higher in dogs as compared to the pooled blood.
  • ACP from both dogs and horses partially inhibited the growth of Enterococcus faecalis, a multi-drug resistant bacteria.
  • However, AMEED did not show any antibacterial effects against the tested bacteria.

Conclusions

  • The study concluded that ACP from both dogs and horses can potentially restrict the growth of Enterococcus faecalis in a controlled environment.
  • As AMEED showed no antibacterial effects, further research with varying concentrations of ACP against different bacterial isolates from corneal ulcers is suggested for future study.

Cite This Article

APA
Yates KM, Stilwell NK, Fontenot RL, Betbeze CM. (2023). In vitro antibacterial efficacy of autologous conditioned plasma and amniotic membrane eye drops. Vet Ophthalmol, 26(6), 548-554. https://doi.org/10.1111/vop.13120

Publication

Veterinary ophthalmology
ISSN: 1463-5224
NlmUniqueID: 100887377
Country: England
Language: English
Volume: 26
Issue: 6
Pages: 548-554

Researcher Affiliations

Yates, Kelsey M
  • Department of Clinical Sciences, Mississippi State University, Mississippi State, Mississippi, USA.
Stilwell, Natalie K
  • Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, Mississippi, USA.
Fontenot, Robin L
  • Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, Mississippi, USA.
Betbeze, Caroline M
  • Department of Clinical Sciences, Mississippi State University, Mississippi State, Mississippi, USA.

MeSH Terms

  • Humans
  • Sheep
  • Animals
  • Horses
  • Dogs
  • Amnion
  • Ulcer / veterinary
  • Cornea
  • Plasma
  • Anti-Bacterial Agents / pharmacology
  • Dog Diseases
  • Horse Diseases
  • Sheep Diseases

Grant Funding

  • 299121-182010-021000-CVM072 / Mississippi State University COllege of Veterinary Medicine Office of Research and Graduate Studies Veterinary Student Research Initiative

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