In vitro effects of lidocaine on the contractility of equine jejunal smooth muscle challenged by ischaemia-reperfusion injury.
Abstract: Post operative ileus (POI) in horses is a severe complication after colic surgery. A commonly used prokinetic drug is lidocaine, which has been shown to have stimulatory effects on intestinal motility. The cellular mechanisms through which lidocaine affects smooth muscle activity are not yet known. Objective: To examine the effects of lidocaine on smooth muscle in vitro and identify mechanisms by which it may affect the contractility of intestinal smooth muscle. Objective: Ischaemia and reperfusion associated with intestinal strangulation can cause smooth muscle injury. Consequently, muscle cell functionality and contractile performance is decreased. Lidocaine can improve basic cell functions and thereby muscle cell contractility especially in ischaemia-reperfusion-challenged smooth muscle. Methods: To examine the effects of lidocaine on smooth muscle function directly, isometric force performance was measured in vitro in noninjured and in vivo ischaemia-reperfusion injured smooth muscle tissues. Dose-dependent response of lidocaine was measured in both samples. To assess membrane permeability as a marker of basic cell function, release of creatine kinase (CK) was measured by in vitro incubations. Results: Lidocaine-stimulated contractility of ischaemia-reperfusion injured smooth muscle was more pronounced than that of noninjured smooth muscle. A 3-phasic dose-dependency was observed with an initial recovery of contractility especially in ischaemia-reperfusion injured smooth muscle followed by a plateau phase where contractility was maintained over a broad concentration range. CK release was decreased by lidocaine. Conclusions: Lidocaine may improve smooth muscle contractility and basic cell function by cellular repair mechanisms which are still unknown. Improving contractility of smooth muscle after ischaemia-reperfusion injury is essential in recovery of propulsive intestinal motility. Conclusions: Characterisation of the cellular mechanisms of effects of lidocaine, especially on ischaemia-reperfusion injured smooth muscle, may lead to improved treatment strategies for horses with POI.
Publication Date: 2010-02-04 PubMed ID: 20121914DOI: 10.2746/042516409X475454Google Scholar: Lookup
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- Journal Article
Summary
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The researchers investigated the impacts of lidocaine on the smooth muscle function of horse intestines, especially under conditions of ischemia-reperfusion injury, which often affects horses after colic surgery. The study revealed that lidocaine enhances muscle contractility, particularly in muscles damaged by ischemia-reperfusion injury, potentially improving recovery prospects after such operations.
Study Intentions
- This study aimed to understand the effects of lidocaine on the smooth muscle of horse intestines, particularly during cases of ischemia-reperfusion injury. This type of injury typically occurs in horses post colic surgery, leading to a severe condition known as post-operative ileus (POI).
- Though the prokinetic effects of lidocaine — its ability to stimulate intestinal motility — are known and the drug is widely used for this purpose, the precise cellular mechanisms through which it performs this function remain elusive. The study sought to shed light on these mechanisms.
Methodology
- The team employed an in vitro approach, directly measuring isometric force performance in both non-injured and ischemia-reperfusion injured smooth muscle tissues to assess the effects of lidocaine.
- They also measured the dose-dependent response of lidocaine in both types of samples, with particular attention placed on membrane permeability — a critical factor in basic cell functionality. This was assessed by measuring the release of creatine kinase (CK) during in vitro incubations.
Findings
- The researchers found that lidocaine stimulation of the contractility of ischemia-reperfusion injured smooth muscle was more noticeable than in non-injured smooth muscle.
- They also discovered a three-phase dose-dependency effect — an initial recovery of contractility was witnessed, specifically in ischemia-reperfusion injured smooth muscle; this was followed by a plateau phase during which contractility was maintained across a broad concentration range of the drug.
- Moreover, the study revealed that CK release — a key sign of healthy cellular function — was reduced by lidocaine.
Implications and Conclusions
- These findings suggest that lidocaine can enhance smooth muscle contractility and basic cell function, likely through unknown cellular repair mechanisms. This could potentially contribute significantly to the recovery of propulsive intestinal motility in horses after surgeries involving ischemia-reperfusion injuries.
- The research underscores the importance of further studies to characterize the cellular mechanisms of lidocaine’s effects, especially on ischemia-reperfusion injured smooth muscle. This could lead to improved treatment strategies for horses with post-operative ileus.
Cite This Article
APA
Guschlbauer M, Hoppe S, Geburek F, Feige K, Huber K.
(2010).
In vitro effects of lidocaine on the contractility of equine jejunal smooth muscle challenged by ischaemia-reperfusion injury.
Equine Vet J, 42(1), 53-58.
https://doi.org/10.2746/042516409X475454 Publication
Researcher Affiliations
- Department of Physiology, University of Veterinary Medicine, Hannover, Germany.
MeSH Terms
- Animals
- Creatine Kinase / metabolism
- Female
- Horses
- Jejunum / pathology
- Lidocaine / pharmacology
- Male
- Muscle Contraction / drug effects
- Muscle Contraction / physiology
- Muscle, Smooth / blood supply
- Muscle, Smooth / pathology
- Reperfusion Injury / pathology
- Reperfusion Injury / veterinary
Citations
This article has been cited 4 times.- Verhaar N, Hoppe S, Grages AM, Hansen K, Neudeck S, Kästner S, Mazzuoli-Weber G. Dexmedetomidine Has Differential Effects on the Contractility of Equine Jejunal Smooth Muscle Layers In Vitro.. Animals (Basel) 2023 Mar 10;13(6).
- Smith JS, Zhou X, Merkatoris PT, Klostermann CA, Breuer RM. Medical Management of Hemorrhagic Bowel Syndrome in a Beef Bull.. Case Rep Vet Med 2019;2019:9209705.
- Schroeder DC, Maul AC, Mahabir E, Koxholt I, Yan X, Padosch SA, Herff H, Bultmann-Mellin I, Sterner-Kock A, Annecke T, Hucho T, Böttiger BW, Guschlbauer M. Evaluation of small intestinal damage in a rat model of 6 Minutes cardiac arrest.. BMC Anesthesiol 2018 Jun 5;18(1):61.
- Stahl J, Kietzmann M. The effects of chemical and physical penetration enhancers on the percutaneous permeation of lidocaine through equine skin.. BMC Vet Res 2014 Jun 20;10:138.
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