In vitro effects of three equimolar concentrations of methylprednisolone acetate, triamcinolone acetonide, and isoflupredone acetate on equine articular tissue cocultures in an inflammatory environment.
Abstract: OBJECTIVE To compare the effects of 3 equimolar concentrations of methylprednisolone acetate (MPA), triamcinolone acetonide (TA), and isoflupredone acetate (IPA) on equine articular tissue cocultures in an inflammatory environment. SAMPLE Synovial and osteochondral explants from the femoropatellar joints of 6 equine cadavers (age, 2 to 11 years) without evidence of musculoskeletal disease. PROCEDURES From each cadaver, synovial and osteochondral explants were harvested from 1 femoropatellar joint to create cocultures. Cocultures were incubated for 96 hours with (positive control) or without (negative control) interleukin (IL)-1β (10 ng/mL) or with IL-1β and MPA, TA, or IPA at a concentration of 10, 10, or 10M. Culture medium samples were collected from each coculture after 48 and 96 hours of incubation. Concentrations of prostaglandin E, matrix metalloproteinase-13, lactate dehydrogenase, and glycosaminoglycan were determined and compared among treatments at each time. RESULTS In general, low concentrations (10 and 10M) of MPA, TA, and IPA mitigated the inflammatory and catabolic (as determined by prostaglandin E and matrix metalloproteinase-13 quantification, respectively) effects of IL-1β in cocultures to a greater extent than the high (10M) concentration. Mean culture medium lactate dehydrogenase concentration for the 10M IPA treatment was significantly greater than that for the positive control at both times, which was suggestive of cytotoxicosis. Mean culture medium glycosaminoglycan concentration did not differ significantly. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that the in vitro effects of IPA and MPA were similar to those of TA at clinically relevant concentrations (10 and 10M).
Publication Date: 2018-08-29 PubMed ID: 30153056DOI: 10.2460/ajvr.79.9.933Google Scholar: Lookup
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- Journal Article
Summary
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The research compares the effects of three comparable concentrations of methylprednisolone acetate, triamcinolone acetonide, and isoflupredone acetate on horse joint tissue in an environment with inflammation.
Objective and Sample Used
- The aim of the study was to evaluate and contrast the impacts of three similar concentrations of methylprednisolone acetate (MPA), triamcinolone acetonide (TA), and isoflupredone acetate (IPA) on horse joint tissue in an inflammatory setting.
- The sample comprised of synovial and osteochondral explants taken from the femoropatellar joints of six horse cadavers ranging from 2 to 11 years old. The selected animals showed no signs of musculoskeletal disease.
Procedure and Methods
- The researchers formed cocultures from harvested synovial and osteochondral explants. These are mixtures of tissues taken from the joint, cultured together to form a more natural environment for the cells.
- These cocultures were then incubated for 96 hours in different settings: with interleukin (IL)-1β, which stimulates inflammation; without IL-1β; and with IL-1β plus each of the three corticosteroids (MPA, TA, IPA) at three concentrations.
- Researchers collected samples from the medium in which the tissues were cultured after 48 hours and 96 hours of incubation.
- They assessed the amounts of prostaglandin E (an inflammatory marker), matrix metalloproteinase-13 (which can degrade joint tissue), lactate dehydrogenase (a marker of cell damage), and glycosaminoglycan (a compound found in cartilage).
Results and Conclusion
- Generally, low concentrations of the corticosteroids (MPA, TA, IPA) were more effective at mitigating the inflammatory and catabolic effects of IL-1β in the cocultures than the high concentration.
- The mean lactate dehydrogenase concentration in the medium for the high concentration IPA treatment was significantly greater than that for the positive control, suggesting that IPA may be toxic to cells at this level.
- There was no statistically significant difference in glycosaminoglycan concentration across treatments.
- The results thus suggest that MPA and IPA could have effects comparable to those of TA in clinically relevant concentrations.
Cite This Article
APA
Trahan RA, Byron CR, Dahlgren LA, Pleasant RS, Werre SR.
(2018).
In vitro effects of three equimolar concentrations of methylprednisolone acetate, triamcinolone acetonide, and isoflupredone acetate on equine articular tissue cocultures in an inflammatory environment.
Am J Vet Res, 79(9), 933-940.
https://doi.org/10.2460/ajvr.79.9.933 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cartilage, Articular / drug effects
- Cartilage, Articular / metabolism
- Coculture Techniques
- Dinoprostone / metabolism
- Female
- Fluprednisolone / administration & dosage
- Fluprednisolone / analogs & derivatives
- Glycosaminoglycans / metabolism
- Horses
- Inflammation
- Injections, Intra-Articular
- Interleukin-1beta / metabolism
- L-Lactate Dehydrogenase / metabolism
- Male
- Matrix Metalloproteinase 13 / metabolism
- Methylprednisolone / administration & dosage
- Methylprednisolone / analogs & derivatives
- Methylprednisolone Acetate
- Osteoarthritis / drug therapy
- Osteoarthritis / veterinary
- Triamcinolone Acetonide / administration & dosage
Citations
This article has been cited 3 times.- Mobasheri A, Trumble TN, Byron CR. Editorial: One Step at a Time: Advances in Osteoarthritis. Front Vet Sci 2021;8:727477.
- Quam VG, Belacic ZA, Long S, Rice HC, Dhar MS, Durgam S. Equine bone marrow MSC-derived extracellular vesicles mitigate the inflammatory effects of interleukin-1β on navicular tissues in vitro. Equine Vet J 2025 Jan;57(1):232-242.
- Kantasrila R, Pandith H, Balslev H, Wangpakapattanawong P, Panyadee P, Inta A. Ethnobotany and phytochemistry of plants used to treat musculoskeletal disorders among Skaw Karen, Thailand. Pharm Biol 2024 Dec;62(1):62-104.
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