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Acta veterinaria Scandinavica2001; 42(2); 199-217; doi: 10.1186/1751-0147-42-199

In vitro evaluation of frozen-thawed stallion semen: a review.

Abstract: The article reviews methods used for in vitro evaluation of sperm, with particular emphasis on frozen-thawed stallion sperm. The techniques, limitations of the methods and correlations with fertility results are discussed. Very few studies have tried to find correlation between fertility of frozen stallion semen and laboratory tests. It is difficult and expensive to inseminate an adequate number of mares to achieve statistically significant differences. Significant, but low correlations have been demonstrated between the foaling rate and subjective motility of sperm incubated for 2 h and 4 h at 37 degrees C and hypoosmotic swelling test after 0 and 3 h of incubation. Significant correlations have been reported between the pregnancy rate and viability of propidium iodide-stained sperm assessed by flow cytometry as well as for glass wool and Sephadex filtration tests. No correlations have been detected between fertility and motility immediately after thawing. In spite of that, motility estimation by light microscope is the most commonly used method to evaluate frozen-thawed stallion sperm. Computer assisted automatic sperm analyzers have replaced light microscopy in research projects, but so far nobody has been able to demonstrate a correlation between fertility of frozen stallion semen and any of the motility parameters obtained by these instruments.
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Publication Date: 2001-08-16 PubMed ID: 11503365PubMed Central: PMC2202319DOI: 10.1186/1751-0147-42-199Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article reviews the methods of in vitro evaluation of frozen-thawed stallion sperm and discusses their limitations, techniques, and correlations with fertility results. However, correlating laboratory tests with the fertility of frozen stallion semen proves complex due to the costly and intricate process of inseminating a sufficient number of mares to achieve statistical significance.

Evaluation Techniques and Limitations

  • The techniques used for evaluating frozen-thawed stallion sperm vary widely. Some methods include subjective motility and hypoosmotic swelling tests, the viability of propidium iodide-stained sperm assessed by flow cytometry, and the use of glass wool and Sephadex filtration tests.
  • The limitations of these methods include their complexity, lack of a high correlation with fertility results, and the cost associated with the insemination of a large number of mares for statistical significance.
  • Moreover, motility assessment immediately after thawing doesn’t correlate with fertility, challenge the reliability of this commonly used method in evaluating frozen-thawed stallion sperm.

Correlation with Fertility Results

  • Some significant, albeit low, correlations have been found between fertility rates and subjective motility of sperm incubated for 2 and 4 hours at 37 degrees Celsius.
  • Significant correlations have also been reported between pregnancy rates and the viability of propidium iodide-stained sperm assessed by flow cytometry as well as results from glass wool and Sephadex filtration tests.
  • Despite the evidence supporting these methods, it has proven difficult to establish a direct correlation between these laboratory tests and the fertility of frozen stallion semen.

Modern Evaluation Techniques

  • In modern research projects, computer-assisted automatic sperm analyzers have replaced traditional light microscopy in the evaluation of sperm motility. However, these automated systems have yet to prove a correlative link between their motility parameter results and the fertility of frozen stallion semen.
  • The challenges with these methods indicate a persisting need for further research and advances in technology to improve the accuracy of evaluating frozen-thawed stallion sperm fertility.

Cite This Article

APA
Katila T. (2001). In vitro evaluation of frozen-thawed stallion semen: a review. Acta Vet Scand, 42(2), 199-217. https://doi.org/10.1186/1751-0147-42-199

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 42
Issue: 2
Pages: 199-217

Researcher Affiliations

Katila, T
  • Department of Clinical Veterinary Sciences, University of Helsinki, Finland. tkatila@saari.vetmed.helsinki.fi

MeSH Terms

  • Animals
  • Cryopreservation
  • Female
  • Fertility
  • Horses
  • Male
  • Semen Preservation
  • Sperm Motility

References

This article includes 93 references
  1. Abraham-Peskir JV, Chantler E, Uggerhøj E. Significance of plasmalemma disruption in bovine and equine spermatozoa.. Theriogenology 2000 Oct 15;54(7):1075-86.
    doi: 10.1016/S0093-691X(00)00416-7pubmed: 11131326google scholar: lookup
  2. Agarwal A, Ozturk E, Loughlin KR. Comparison of semen analysis between the two Hamilton-Thorn semen analysers.. Andrologia 1992 Nov-Dec;24(6):327-9.
    pubmed: 1443674doi: 10.1111/j.1439-0272.1992.tb02663.xgoogle scholar: lookup
  3. Amann RP. Can the fertility potential of a seminal sample be predicted accurately?. J Androl 1989 Mar-Apr;10(2):89-98.
    pubmed: 2715106doi: 10.1002/j.1939-4640.1989.tb00066.xgoogle scholar: lookup
  4. Ball BA, Fagnan MS, Dobrinski I. Determination of acrosin amidase activity in equine spermatozoa.. Theriogenology 1997 Nov;48(7):1191-8.
    doi: 10.1016/S0093-691X(97)00352-Xpubmed: 16728208google scholar: lookup
  5. Bataille B, Magistrini M, Palmer E. Analyse objective de la mobilite du sperme congele-decongele d'etalon. Essai decorrelation avec la fertilite. (Objective determination of sperm motility in frozen-thawed stallion semen. Correlation with fertility). Anim Breed Abstr 1990 pp. 96–106.
  6. Bedford SJ, Varner DD, Meyers SA. Effects of cryopreservation on the acrosomal status of stallion spermatozoa.. J Reprod Fertil Suppl 2000;(56):133-40.
    pubmed: 20681125
  7. Bielański W. The evaluation of stallion semen in aspects of fertility control and its use for artificial insemination.. J Reprod Fertil Suppl 1975 Oct;(23):19-24.
    pubmed: 1060777
  8. Bielański W, Kaczmarski F. Morphology of spermatozoa in semen from stallions of normal fertility.. J Reprod Fertil Suppl 1979;(27):39-45.
    pubmed: 289815
  9. Blach EL, Amann RP, Bowen RA, Frantz D. Changes in quality of stallion spermatozoa during cryopreservation: Plasma membrane integrity and motion characteristics.. Theriogenology 1989 Feb;31(2):283-98.
    doi: 10.1016/0093-691X(89)90533-5pubmed: 16726547google scholar: lookup
  10. Blanc G, Magistrini M, Palmer E. Use of concanavalin A for coating the membranes of stallion spermatozoa.. J Reprod Fertil Suppl 1991;44:191-8.
    pubmed: 1795261
  11. Borg K, Colenbrander B, Fazeli A, Parlevliet J, Malmgren L. Influence of thawing method on motility, plasma membrane integrity and morphology of frozen-thawed stallion spermatozoa.. Theriogenology 1997 Sep;48(4):531-6.
    doi: 10.1016/S0093-691X(97)00269-0pubmed: 16728148google scholar: lookup
  12. Boyle MS. Artificial insemination: assessing stallion semen quality after freezing.. Equine Vet J 1996 Jan;28(1):5-6.
    pubmed: 8565954doi: 10.1111/j.2042-3306.1996.tb01580.xgoogle scholar: lookup
  13. Brown KI, Crabo BG, Graham EF, Pace MM. Some factors affecting loss of intracellular enzymes from spermatozoa.. Cryobiology 1971 Apr;8(2):220-4.
    doi: 10.1016/0011-2240(71)90030-7pubmed: 5578886google scholar: lookup
  14. Budworth PR, Amann RP, Chapman PL. Relationships between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility.. J Androl 1988 Jan-Feb;9(1):41-54.
    pubmed: 3346179doi: 10.1002/j.1939-4640.1988.tb01007.xgoogle scholar: lookup
  15. Burns PJ, Reasner DS. Computerized analysis of sperm motion: effects of glycerol concentration on the cryopreservation of equine spermatozoa. J Equine Vet Sci 1995;15:377–380.
    doi: 10.1016/S0737-0806(07)80480-1google scholar: lookup
  16. Caiza de la Cueva FI, Pujol MR, Rigau T, Bonet S, Miró J, Briz M, Rodriguez-Gill JE. Resistance to osmotic stress of horse spermatozoa: the role of ionic pumps and their relationship to cryopreservation success.. Theriogenology 1997 Oct 15;48(6):947-68.
    doi: 10.1016/S0093-691X(97)00322-1pubmed: 16728185google scholar: lookup
  17. Casey PJ, Hillman RB, Robertson KR, Yudin AI, Liu IK, Drobnis EZ. Validation of an acrosomal stain for equine sperm that differentiates between living and dead sperm.. J Androl 1993 Jul-Aug;14(4):289-97.
    pubmed: 7693637
  18. Cheng F-P. The acrosome reaction in stallion spermatozoa. Thesis, Utrecht. 1997.
  19. Cheng FP, Fazeli A, Voorhout WF, Marks A, Bevers MM, Colenbrander B. Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa.. J Androl 1996 Nov-Dec;17(6):674-82.
    pubmed: 9016398
  20. Christensen P, Parlevliet JM, van Buiten A, Hyttel P, Colenbrander B. Ultrastructure of fresh and frozen-thawed stallion spermatozoa. Biol Reprod 1995;Mono 1:769–777.
  21. Clarke RN, Johnson LA. Effect of liquid storage and cryopreservation of boar spermatozoa on acrosomal integrity and the penetration of zona-free hamster ova in vitro.. Gamete Res 1987 Mar;16(3):193-204.
    doi: 10.1002/mrd.1120160302pubmed: 3506910google scholar: lookup
  22. Colenbrander B, Fazeli AR, van Buiten A, Parlevliet J, Gadella BM. Assessment of sperm cell membrane integrity in the horse.. Acta Vet Scand Suppl 1992;88:49-58.
    pubmed: 1283655
  23. Comhaire FH, Huysse S, Hinting A, Vermeulen L, Schoonjans F. Objective semen analysis: has the target been reached?. Hum Reprod 1992 Feb;7(2):237-41.
    pubmed: 1577938doi: 10.1093/oxfordjournals.humrep.a137624google scholar: lookup
  24. Dart MG, Mesta J, Crenshaw C, Ericsson SA. Modified resazurin reduction test for determining the fertility potential of bovine spermatozoa.. Arch Androl 1994 Sep-Oct;33(2):71-5.
    doi: 10.3109/01485019408987806pubmed: 7818374google scholar: lookup
  25. Davis RO, Gravance CG, Casey PJ. Automated morphometric analysis of stallion spermatozoa.. Am J Vet Res 1993 Nov;54(11):1808-11.
    pubmed: 8291755
  26. De Albuquerque Lagares M. Bestimmung der osmotischen Resistenz von Hengstsamenzellen (The determination of the osmotic resistance of stallion spermatozoa). Thesis, Hannover. 1995.
  27. Dowsett KF, Pattie WA. Characteristics and fertility of stallion semen.. J Reprod Fertil Suppl 1982;32:1-8.
    pubmed: 6962847
  28. Drevius LO, Eriksson H. Osmotic swelling of mammalian spermatozoa.. Exp Cell Res 1966 Apr;42(1):136-56.
    doi: 10.1016/0014-4827(66)90327-2pubmed: 5948776google scholar: lookup
  29. Eriksson B, Juonala T, Andersson M, Rodriguez-Martines H. Viability of frozen-thawed boar semen. Proc 50th Anniv ICAR 1998 p. 526.
  30. Evenson DP, Thompson L, Jost L. Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility.. Theriogenology 1994 Feb 2;41(3):637-51.
    doi: 10.1016/0093-691X(94)90174-Hpubmed: 16727419google scholar: lookup
  31. Farlin ME, Jasko DJ, Graham JK, Squires EL. Assessment of Pisum sativum agglutinin in identifying acrosomal damage in stallion spermatozoa.. Mol Reprod Dev 1992 May;32(1):23-7.
    doi: 10.1002/mrd.1080320105pubmed: 1515146google scholar: lookup
  32. Farrell PB, Foote RH, Zinaman MJ. Motility and other characteristics of human sperm can be measured by computer-assisted sperm analysis of samples stained with Hoechst 33342.. Fertil Steril 1996 Sep;66(3):446-53.
    pubmed: 8751746
  33. Fazeli AR, Steenweg W, Bevers MM, van den Broek J, Bracher V, Parlevliet J, Colenbrander B. Relation between stallion sperm binding to homologous hemizonae and fertility.. Theriogenology 1995 Oct;44(5):751-60.
    doi: 10.1016/0093-691X(95)00254-6pubmed: 16727772google scholar: lookup
  34. Glander HJ, Herold W. Influence of cryopreservation on the microelectrophoretic motility (EPM) of human spermatozoa.. Andrologia 1991 Jul-Aug;23(4):263-7.
    pubmed: 1772138doi: 10.1111/j.1439-0272.1991.tb02556.xgoogle scholar: lookup
  35. Graham JK. Response of sperm to freezing and analysis of sperm. Proc Ann Meet Soc Theriog 1997 pp. 170–177.
  36. Gravance CG, Garner DL, Baumber J, Ball BA. Assessment of equine sperm mitochondrial function using JC-1.. Theriogenology 2000 Jun;53(9):1691-703.
    doi: 10.1016/S0093-691X(00)00308-3pubmed: 10968415google scholar: lookup
  37. Hammitt DG, Martin PA. Correlations among assays of porcine semen quality following cryopreservation.. Theriogenology 1989 Sep;32(3):369-84.
    doi: 10.1016/0093-691X(89)90004-6pubmed: 16726684google scholar: lookup
  38. Hermenet MJ, Sawyer HR, Pickett BW, Amann RP, Squires EL, Long PL. Effect of stain, technician, number of spermatozoa evaluated and slide preparation on assessment of spermatozoal viability by light microscopy. J Equine Vet Sci 1993;13:449–455.
    doi: 10.1016/S0737-0806(07)80283-8google scholar: lookup
  39. Jasko DJ. Evaluation of stallion semen.. Vet Clin North Am Equine Pract 1992 Apr;8(1):129-48.
    pubmed: 1576546doi: 10.1016/s0749-0739(17)30471-6google scholar: lookup
  40. Jasko DJ, Lein DH, Foote RH. Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987-1988).. J Am Vet Med Assoc 1990 Aug 1;197(3):389-94.
    pubmed: 2391279
  41. Jasko DJ, Lein DH, Foote RH. A comparison of two computer-automated semen analysis instruments for the evaluation of sperm motion characteristics in the stallion.. J Androl 1990 Sep-Oct;11(5):453-9.
    pubmed: 2254179
  42. Jasko DJ, Little TV, Lein DH, Foote RH. Comparison of spermatozoal movement and semen characteristics with fertility in stallions: 64 cases (1987-1988).. J Am Vet Med Assoc 1992 Apr 1;200(7):979-85.
    pubmed: 1577655
  43. Katila T, Kuisma P, Andersson M. Evaluation of frozen stallion semen. In: Allen WR, Wade JF, editor. Proceeding of the First Meeting of the European Equine Gamete Group (EEGG) 1. Havemeyer Foundation Monograph Series; 2000. pp. 19–21a.
  44. Katila T, Koskinen E, Andersson M. Evaluation of frozen-thawed semen. A Dorothy Russell Havemeyer Foundation Workshop Advanced Current Topics in Stallion Veterinary Practice. 2000. pp. 54–56b.
  45. Kenney RM, Evenson DP, Garcia MC, Love CC. Relationships between sperm chromatin structure, motility, and morphology of ejaculated sperm, and seasonal pregnancy rate. Biol Reprod 1995;Mono 1:647–653.
  46. Kenney RM, Hurtgen J, Pierson R, Witherspoon D, Simons J. Theriogenology and the equine, part II: the stallion. J Soc Theriog 1893 p. 9.
  47. Kneissl S. Tiefgefrierkonservierung von Pferdesperma: Einfluss der Samenentnahmetechnik, Zentrifugation, Konfektionierungsform und Einfriermethode auf die Motilität und Membranintegrität der Samenzellen. (Cryopreservation of stallion semen: The influence of semen collection techniques, centrifugation, packaging forms, and methods of freezing on the motility and plasma membrane integrity of spermatozoa). Thesis Hannover. 1993.
  48. Kosiniak K. The evaluation of stallion semen in aspects of freezability control and its use for practice. Egypt J Vet Sci 1988;25:1–19.
  49. Larsson B, Andersson M, Aalto J, Shamsuddin M, Rodriguez-Martinez H. A comparison between different methods for estimation of the fertility in AI young bulls. XVII Nordic Vet Congr Reykjavik, Iceland. 1994. pp. 216–217.
  50. Loomis P. Artificial insemination of horses: Where is it going?. Soc Theriogenology, Proc Ann Conf Nashville, Tennessee, Sept 22–24, 1999 pp. 325–336.
  51. Magistrini M, Guitton E, Levern Y, Nicolle JC, Vidament M, Kerboeuf D, Palmer E. New staining methods for sperm evaluation estimated by microscopy and flow cytometry.. Theriogenology 1997 Nov;48(7):1229-35.
    doi: 10.1016/S0093-691X(97)00355-5pubmed: 16728211google scholar: lookup
  52. Malchow NM, Arns MJ. Influence of salt storage on equine zonae pellucidae: electrophoretic properties and interaction with spermatozoa. Biol Reprod 1995;Mono 1:671–679.
  53. Malmgren L. Assessing the quality of raw semen: a review.. Theriogenology 1997 Sep;48(4):523-30.
    doi: 10.1016/S0093-691X(97)00268-9pubmed: 16728147google scholar: lookup
  54. Mennick PE. Equine semen cryopreservation: An update. Soc Theriogenology Proc Ann Meeting, September 17–20, 1997, Montreal, Q, Canada pp. 161–169.
  55. Meyers SA, Liu IK, Overstreet JW, Vadas S, Drobnis EZ. Zona pellucida binding and zona-induced acrosome reactions in horse spermatozoa: comparisons between fertile and subfertile stallions.. Theriogenology 1996 Nov 1;46(7):1277-88.
    doi: 10.1016/S0093-691X(96)00299-3pubmed: 16727991google scholar: lookup
  56. Meyers SA, Overstreet JW, Liu IK, Drobnis EZ. Capacitation in vitro of stallion spermatozoa: comparison of progesterone-induced acrosome reactions in fertile and subfertile males.. J Androl 1995 Jan-Feb;16(1):47-54.
    pubmed: 7768752
  57. Młodawska W, Palmer E, Duchamp G, Okólski A, Bézard J. Zona pellucida-sperm binding assay for equine oocytes.. J Reprod Fertil Suppl 2000;(56):423-9.
    pubmed: 20681155
  58. Morrell JM. Applications of flow cytometry to artificial insemination: a review.. Vet Rec 1991 Oct 26;129(17):375-8.
    pubmed: 1720909doi: 10.1136/vr.129.17.375google scholar: lookup
  59. Morrow A, Drudy L, Gordon A, Harrison RF. Evaluation of bovine cervical mucus penetration as a test of human spermatozoal function for an in vitro fertilization programme.. Andrologia 1992 Nov-Dec;24(6):323-6.
    pubmed: 1443673doi: 10.1111/j.1439-0272.1992.tb02662.xgoogle scholar: lookup
  60. Müller Z. Fertility of frozen equine semen.. J Reprod Fertil Suppl 1982;32:47-51.
    pubmed: 6962886
  61. Müller Z. Practicalities of insemination of mares with deep-frozen semen.. J Reprod Fertil Suppl 1987;35:121-5.
    pubmed: 3479567
  62. Neild D, Chaves G, Flores M, Mora N, Beconi M, Agüero A. Hypoosmotic test in equine spermatozoa.. Theriogenology 1999 Mar;51(4):721-7.
    doi: 10.1016/S0093-691X(99)00021-7pubmed: 10728997google scholar: lookup
  63. Nie GJ, Wenzel JG. Adaptation of the hypoosmotic swelling test to assess functional integrity of stallion spermatozoal plasma membranes.. Theriogenology 2001 Mar 1;55(4):1005-18.
    doi: 10.1016/S0093-691X(01)00461-7pubmed: 11291907google scholar: lookup
  64. Oettle EE. Using a new acrosome stain to evaluate sperm morphology. Vet Med 1986;81:263–266.
  65. Okólski A, Babusik P, Tischner M, Lietz W. Evaluation of mare oocyte collection methods and stallion sperm penetration of zona-free hamster ova.. J Reprod Fertil Suppl 1987;35:191-6.
    pubmed: 3479575
  66. Padilla AW, Tobback C, Foote RH. Penetration of frozen-thawed, zona-free hamster oocytes by fresh and slow-cooled stallion spermatozoa.. J Reprod Fertil Suppl 1991;44:207-12.
    pubmed: 1795263
  67. Palmer E, Magistrini M. Automated analysis of stallion semen post-thaw motility.. Acta Vet Scand Suppl 1992;88:137-52.
    pubmed: 1285536
  68. Pantke P, Hyland JH, Galloway DB, Liu DY, Baker HWG. Development of a zona pellucida sperm binding assay for the assessment of stallion fertility. Biol Reprod 1995;Mono 1:681–687.
  69. Papaioannou KZ, Murphy RP, Monks RS, Hynes N, Ryan MP, Boland MP, Roche JF. Assessment of viability and mitochondrial function of equine spermatozoa using double staining and flow cytometry.. Theriogenology 1997 Jul 15;48(2):299-312.
    doi: 10.1016/S0093-691X(97)84077-0pubmed: 16728129google scholar: lookup
  70. Parlevliet JM, Fazeli AR, Steenweg W, Bevers MM, Colenbrander B. The effect of storage methods on the sperm zona binding in the horse. 6th Int Symp Equine Reprod, Caxambu, Brazil, Programme and miniposters. 1994. pp. 171–172.
  71. Pickett BW, Squires EL, McKinnon AO. Procedures for collection, evaluation and utilization of stallion semen for artificial insemination. Anim Reprod Lab. Colorado State University, Fort Collins 1987.
  72. Rana N, Jeyendran RS, Holmgren WJ, Rotman C, Zaneveld LJ. Glass wool-filtered spermatozoa and their oocyte penetrating capacity.. J In Vitro Fert Embryo Transf 1989 Oct;6(5):280-4.
    doi: 10.1007/BF01139182pubmed: 2632658google scholar: lookup
  73. Rathi R, Nielen M, Cheng FP, van Buiten A, Colenbrander B. Exposure of progesterone receptors on the plasma membranes of stallion spermatozoa as a parameter for prediction of fertility.. J Reprod Fertil Suppl 2000;(56):87-91.
    pubmed: 20681119
  74. Renner C, Gehring W, Holzmann A, Failing K. Korrelationen spermabiologischer Merkmale in nativen undaufgetauten Hengstejakulaten unter Berücksichtigung derTransmigration. (Correlations of semen characters in fresh and thawedstallion ejaculates, with consideration of transmigration). Reprod Dom Anim 1992;27:148–153.
  75. Risse S. Die bisherigen Möglichkeiten zur Einschätzung der Qualität und Fertilität von Spermienzellen. (Existing possibilities for evaluating sperm quality and fertility). Monatshefte Vet Med 1990;45:348–352.
  76. Rodriguez H, Bustos Obregon E. Seasonality and freezability vs routine parameters in stallion semen.. Histol Histopathol 1996 Apr;11(2):427-30.
    pubmed: 8861765
  77. Samper JC, Crabo BG. Assay of capacitated, freeze-damaged and extended stallion spermatozoa by filtration. Theriogenology 1993;39:1209–1220.
    doi: 10.1016/0093-691X(93)90224-Sgoogle scholar: lookup
  78. Samper JC, Hellander JC, Crabo BG. Relationship between the fertility of fresh and frozen stallion semen and semen quality.. J Reprod Fertil Suppl 1991;44:107-14.
    pubmed: 1795253
  79. Schrop H. Einfluss der Membranprotektiva Lecithin und Phenylmethansulfonylfluorid auf Motilität und Akrosomenintegrität im aufgetauten Samen; Beurteilung mittels indirekter Immunfluoreszenz im Vergleich zur Spermac-Färbung. (The membrane protective influence of lecithine and phenylmethansulfonyl fluoride on motility and acrosomal integrity of the frozen-thawed equine semen). Thesis, Hannover. 1992.
  80. Squires EL, Amann RP, Pickett BW. Preservation of stallion semen – Colorado experience. Symp Equine Reprod, Denmark 1987 pp. 49–54.
  81. Tischner M. Evaluation of deep-frozen semen in stallions.. J Reprod Fertil Suppl 1979;(27):53-9.
    pubmed: 289832
  82. Van Huffel XM, Varner DD, Hinrichs K, Garcia MC, Strzemienski PJ, Kenney RM. Photomicrographic evaluation of stallion spermatozoal motility characteristics.. Am J Vet Res 1985 Jun;46(6):1272-5.
    pubmed: 4026005
  83. Varner DD, Bowen JA, Johnson L. Effect of heparin on capacitation/acrosome reaction of equine sperm.. Arch Androl 1993 Nov-Dec;31(3):199-207.
    doi: 10.3109/01485019308988400pubmed: 8274046google scholar: lookup
  84. Varner DD, Schumacher J, Blanchard TL, Johnson L. Diseases and Management of Breeding Stallions. American Veterinary Publications, Goleta, CA; 1991.
  85. Varner DD, Vaughan SD, Johnson L. Use of a computerized system for evaluation of equine spermatozoal motility.. Am J Vet Res 1991 Feb;52(2):224-30.
    pubmed: 2012333
  86. Vidament M, Cognard E, Yvon J-M, Sattler M, Palmer E, Magistrini M. Evaluation of stallion semen before and after freezing. Reprod Dom Anim 1998;33:271–277.
    doi: 10.1111/j.1439-0531.1998.tb01357.xgoogle scholar: lookup
  87. Vidament M, Dupere AM, Julienne P, Evain A, Noue P, Palmer E. Equine frozen semen: freezability and fertility field results.. Theriogenology 1997 Oct 15;48(6):907-17.
    doi: 10.1016/S0093-691X(97)00319-1pubmed: 16728182google scholar: lookup
  88. Vieira RC. Akrosinbestimmung an Pferfespermien unter Berücksichtigung bestimmter andrologischer Fragestellungen. (Acrosin determination on equine spermatozoa). Thesis, Hannover. 1980.
  89. Voss JL, Pickett BW, Squires EL. Stallion spermatozoal morphology and motility and their relationships to fertility.. J Am Vet Med Assoc 1981 Feb 1;178(3):287-9.
    pubmed: 7228787
  90. Wilhelm KM, Graham JK, Squires EL. Comparison of the fertility of cryopreserved stallion spermatozoa with sperm motion analyses, flow cytometric evaluation, and zona-free hamster oocyte penetration.. Theriogenology 1996 Sep;46(4):559-78.
    doi: 10.1016/0093-691X(96)00209-9pubmed: 16727923google scholar: lookup
  91. Wöckener A, Schuberth HJ. Freezing of maiden stallion semen-motility and morphological findings in sperm cells assessed by various staining methods including a monoclonal antibody with reactivity against an antigen in the acrosomal ground substance. Reprod Dom Anim 1993;28:265–272.
    doi: 10.1111/j.1439-0531.1993.tb00995.xgoogle scholar: lookup
  92. Yavetz H, Hauser R, Yogev L, Botchan A, Lessing JB, Homonnai ZT, Paz G. Advanced methods for evaluation of sperm quality.. Andrologia 1995 Jan-Feb;27(1):31-5.
    pubmed: 7755187doi: 10.1111/j.1439-0272.1995.tb02092.xgoogle scholar: lookup
  93. Ziegler R. Computervideomikrographische Beurteilung von Hengstsperma vor und nach der Tiefgefrierkonservierung mit eidotterhaltigem Verdünner. (Computerised videomicrographic evaluation of stallion semen before and after freezing in a diluent containing egg yolk). Thesis, Hannover. 1991.

Citations

This article has been cited 7 times.
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    doi: 10.3390/vetsci10030173pubmed: 36977212google scholar: lookup
  2. Gimeno BF, Bariani MV, Laiz-Quiroga L, Martínez-León E, Von-Meyeren M, Rey O, Mutto AÁ, Osycka-Salut CE. Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status. Animals (Basel) 2021 Jan 3;11(1).
    doi: 10.3390/ani11010074pubmed: 33401609google scholar: lookup
  3. Alamaary MS, Haron AW, Hiew MWH, Ali M. Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post-thawed stallion semen. Vet Med Sci 2020 Nov;6(4):666-672.
    doi: 10.1002/vms3.315pubmed: 32602662google scholar: lookup
  4. Delgado-Bermúdez A, Noto F, Bonilla-Correal S, Garcia-Bonavila E, Catalán J, Papas M, Bonet S, Miró J, Yeste M. Cryotolerance of Stallion Spermatozoa Relies on Aquaglyceroporins rather than Orthodox Aquaporins. Biology (Basel) 2019 Nov 12;8(4).
    doi: 10.3390/biology8040085pubmed: 31726707google scholar: lookup
  5. Kondracki S, Wysokińska A, Kania M, Górski K. Application of Two Staining Methods for Sperm Morphometric Evaluation in Domestic Pigs. J Vet Res 2017 Sep;61(3):345-349.
    doi: 10.1515/jvetres-2017-0045pubmed: 29978093google scholar: lookup
  6. Kuisma P, Andersson M, Koskinen E, Katila T. Fertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methods. Acta Vet Scand 2006 Aug 17;48(1):14.
    doi: 10.1186/1751-0147-48-14pubmed: 16987393google scholar: lookup
  7. Boni R, Ruggiero R, De Luca F, Serritella ML, Di Palma T, Cecchini Gualandi S. Repeatability of Selected Parameters Related to Stallion Sperm Quality and Cryotolerance. Animals (Basel) 2025 Sep 26;15(19).
    doi: 10.3390/ani15192805pubmed: 41096400google scholar: lookup
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