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Home / Equine Research Bank / Acta Vet Scand / In vitro evaluation of frozen-thawed stallion semen: a revie...
Acta veterinaria Scandinavica2001; 42(2); 199-217; doi: 10.1186/1751-0147-42-199

In vitro evaluation of frozen-thawed stallion semen: a review.

Abstract: The article reviews methods used for in vitro evaluation of sperm, with particular emphasis on frozen-thawed stallion sperm. The techniques, limitations of the methods and correlations with fertility results are discussed. Very few studies have tried to find correlation between fertility of frozen stallion semen and laboratory tests. It is difficult and expensive to inseminate an adequate number of mares to achieve statistically significant differences. Significant, but low correlations have been demonstrated between the foaling rate and subjective motility of sperm incubated for 2 h and 4 h at 37 degrees C and hypoosmotic swelling test after 0 and 3 h of incubation. Significant correlations have been reported between the pregnancy rate and viability of propidium iodide-stained sperm assessed by flow cytometry as well as for glass wool and Sephadex filtration tests. No correlations have been detected between fertility and motility immediately after thawing. In spite of that, motility estimation by light microscope is the most commonly used method to evaluate frozen-thawed stallion sperm. Computer assisted automatic sperm analyzers have replaced light microscopy in research projects, but so far nobody has been able to demonstrate a correlation between fertility of frozen stallion semen and any of the motility parameters obtained by these instruments.
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Publication Date: 2001-08-16 PubMed ID: 11503365PubMed Central: PMC2202319DOI: 10.1186/1751-0147-42-199Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article reviews the methods of in vitro evaluation of frozen-thawed stallion sperm and discusses their limitations, techniques, and correlations with fertility results. However, correlating laboratory tests with the fertility of frozen stallion semen proves complex due to the costly and intricate process of inseminating a sufficient number of mares to achieve statistical significance.

Evaluation Techniques and Limitations

  • The techniques used for evaluating frozen-thawed stallion sperm vary widely. Some methods include subjective motility and hypoosmotic swelling tests, the viability of propidium iodide-stained sperm assessed by flow cytometry, and the use of glass wool and Sephadex filtration tests.
  • The limitations of these methods include their complexity, lack of a high correlation with fertility results, and the cost associated with the insemination of a large number of mares for statistical significance.
  • Moreover, motility assessment immediately after thawing doesn’t correlate with fertility, challenge the reliability of this commonly used method in evaluating frozen-thawed stallion sperm.

Correlation with Fertility Results

  • Some significant, albeit low, correlations have been found between fertility rates and subjective motility of sperm incubated for 2 and 4 hours at 37 degrees Celsius.
  • Significant correlations have also been reported between pregnancy rates and the viability of propidium iodide-stained sperm assessed by flow cytometry as well as results from glass wool and Sephadex filtration tests.
  • Despite the evidence supporting these methods, it has proven difficult to establish a direct correlation between these laboratory tests and the fertility of frozen stallion semen.

Modern Evaluation Techniques

  • In modern research projects, computer-assisted automatic sperm analyzers have replaced traditional light microscopy in the evaluation of sperm motility. However, these automated systems have yet to prove a correlative link between their motility parameter results and the fertility of frozen stallion semen.
  • The challenges with these methods indicate a persisting need for further research and advances in technology to improve the accuracy of evaluating frozen-thawed stallion sperm fertility.

Cite This Article

APA
Katila T. (2001). In vitro evaluation of frozen-thawed stallion semen: a review. Acta Vet Scand, 42(2), 199-217. https://doi.org/10.1186/1751-0147-42-199

Publication

Acta veterinaria Scandinavica
ISSN: 0044-605X
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 42
Issue: 2
Pages: 199-217

Researcher Affiliations

Katila, T
  • Department of Clinical Veterinary Sciences, University of Helsinki, Finland. tkatila@saari.vetmed.helsinki.fi

MeSH Terms

  • Animals
  • Cryopreservation
  • Female
  • Fertility
  • Horses
  • Male
  • Semen Preservation
  • Sperm Motility

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Citations

This article has been cited 7 times.
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